Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N4P55_RS04220 Genome accession   NZ_CP104408
Coordinates   977535..978743 (-) Length   402 a.a.
NCBI ID   WP_262667007.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain Ant01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 972535..983743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4P55_RS04185 (N4P55_04195) - 973804..973977 (-) 174 WP_064450648.1 DUF3094 family protein -
  N4P55_RS04190 (N4P55_04200) - 974068..974697 (-) 630 WP_053254334.1 DUF1780 domain-containing protein -
  N4P55_RS04195 (N4P55_04205) - 974889..975578 (+) 690 WP_064450649.1 energy-coupling factor ABC transporter permease -
  N4P55_RS04200 (N4P55_04210) - 975643..975858 (+) 216 WP_064450650.1 hypothetical protein -
  N4P55_RS04205 (N4P55_04215) yacG 975855..976055 (-) 201 WP_064450651.1 DNA gyrase inhibitor YacG -
  N4P55_RS04210 (N4P55_04220) coaE 976052..976675 (-) 624 WP_064450652.1 dephospho-CoA kinase -
  N4P55_RS04215 (N4P55_04225) pilD 976672..977538 (-) 867 WP_316963683.1 A24 family peptidase Machinery gene
  N4P55_RS04220 (N4P55_04230) pilC 977535..978743 (-) 1209 WP_262667007.1 type II secretion system F family protein Machinery gene
  N4P55_RS04225 (N4P55_04235) - 978946..979359 (+) 414 WP_316963854.1 pilin -
  N4P55_RS04235 (N4P55_04245) - 979651..979956 (+) 306 WP_221293915.1 DUF6388 family protein -
  N4P55_RS04240 (N4P55_04250) nadC 980017..980865 (-) 849 WP_064450657.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N4P55_RS04245 (N4P55_04255) - 981110..983284 (+) 2175 WP_262667009.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43454.97 Da        Isoelectric Point: 9.9314

>NTDB_id=729668 N4P55_RS04220 WP_262667007.1 977535..978743(-) (pilC) [Pseudomonas fluorescens strain Ant01]
MNEAATIYAWEGINRKGRRVSGQTAGHNLALIKAQLRQQGICPGRVRKKTSHLPSFAPPIKPADIALFTRQLATLLKAGI
PLLQAFDIISEGFEHRQMRELIKGLKQEIAAGNNLAAALRKQPRYFDELYCNLIAAGEQAGALETLLERVAVHLEKSQQL
KARIKKAMTYPCAVIVVACLVSAILLIHVVPQFQSLFSGVDGELPGFTLKVIALSEFLQHAWWMVALGLGATIAGLRQAY
RASPGFRHGLDSGLLKVPLAGTLLRKSAVARYARTLSTTFAAGVPLVQALDSVAGATGNGLFKKAIEQMRQDVSTGMQLN
HSMAHSGLFPSMAIQMTAIGEESGTLDRMLEKVAQHFEADVENLVDNLTSLMEPLIMVILGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=729668 N4P55_RS04220 WP_262667007.1 977535..978743(-) (pilC) [Pseudomonas fluorescens strain Ant01]
ATGAATGAAGCTGCAACGATCTACGCCTGGGAAGGCATCAACCGCAAAGGGCGCAGGGTCTCAGGGCAAACCGCAGGCCA
TAACCTTGCGCTGATCAAGGCGCAACTGCGCCAGCAAGGAATCTGCCCCGGCCGCGTGCGCAAAAAAACCTCGCACCTGC
CAAGCTTTGCGCCGCCAATAAAACCGGCAGATATTGCCCTGTTCACCCGCCAGCTGGCGACATTGCTGAAGGCCGGCATA
CCGCTGCTGCAAGCCTTCGACATCATCAGCGAAGGCTTCGAACACCGACAGATGCGCGAGCTGATAAAGGGCTTGAAACA
GGAGATCGCCGCCGGGAACAACCTGGCGGCAGCACTGCGCAAACAACCGCGCTACTTCGATGAACTGTATTGCAACCTGA
TCGCTGCGGGTGAACAGGCCGGTGCCCTGGAAACCTTGCTGGAGCGGGTGGCGGTACACCTGGAAAAAAGCCAGCAGCTC
AAGGCGCGAATCAAAAAGGCCATGACCTACCCCTGCGCAGTCATCGTGGTCGCCTGCCTGGTCAGTGCAATCCTGCTCAT
CCACGTGGTACCGCAGTTTCAGAGCCTTTTTAGCGGGGTCGACGGCGAGCTGCCAGGCTTTACCTTGAAGGTCATTGCCC
TGTCCGAATTCCTGCAACACGCCTGGTGGATGGTCGCGCTGGGCCTGGGCGCTACCATTGCCGGGCTGCGCCAGGCTTAT
CGGGCTTCCCCCGGTTTTCGTCACGGCCTCGATAGCGGTTTGTTGAAGGTGCCCCTGGCAGGCACACTGCTGAGAAAATC
CGCCGTTGCCCGCTACGCCCGTACACTTTCAACGACTTTTGCAGCGGGCGTACCGTTGGTGCAGGCCCTGGATTCCGTGG
CGGGTGCGACAGGCAATGGTTTGTTCAAAAAGGCGATCGAACAGATGCGTCAAGACGTATCCACAGGCATGCAGTTGAAT
CACTCCATGGCGCATAGCGGCCTATTCCCCAGCATGGCGATCCAGATGACAGCGATCGGTGAAGAATCGGGCACCCTGGA
CCGCATGCTGGAAAAAGTTGCCCAGCATTTTGAGGCCGACGTGGAGAATCTGGTCGACAACCTCACCAGCCTGATGGAGC
CGCTCATCATGGTGATATTGGGGGGCATTGTCGGTGCATTGGTGATCGCCATGTACCTGCCGGTCTTCCAGCTGGGGTCG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.209

98.756

0.604
  pilC Acinetobacter baylyi ADP1

53.283

98.507

0.525
  pilC Acinetobacter baumannii D1279779

52.405

98.259

0.515
  pilC Legionella pneumophila strain ERS1305867

50.126

98.756

0.495
  pilC Vibrio cholerae strain A1552

39.547

98.756

0.391
  pilG Neisseria gonorrhoeae MS11

41.099

95.025

0.391
  pilG Neisseria meningitidis 44/76-A

40.314

95.025

0.383
  pilC Vibrio campbellii strain DS40M4

38.384

98.507

0.378
  pilC Thermus thermophilus HB27

37.656

99.751

0.376