Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   N5B55_RS13335 Genome accession   NZ_CP104381
Coordinates   2758470..2759732 (+) Length   420 a.a.
NCBI ID   WP_304538419.1    Uniprot ID   -
Organism   Ralstonia pickettii strain MCR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2753470..2764732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5B55_RS13315 (N5B55_13320) ispB 2753943..2754872 (+) 930 WP_049779105.1 octaprenyl diphosphate synthase -
  N5B55_RS13325 (N5B55_13330) - 2755165..2756439 (+) 1275 WP_178961058.1 HlyC/CorC family transporter -
  N5B55_RS13330 (N5B55_13335) pilB 2756698..2758425 (+) 1728 WP_154208977.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5B55_RS13335 (N5B55_13340) pilG 2758470..2759732 (+) 1263 WP_304538419.1 type II secretion system F family protein Machinery gene
  N5B55_RS13340 (N5B55_13345) pilD 2759732..2760613 (+) 882 WP_304538420.1 A24 family peptidase Machinery gene
  N5B55_RS13345 (N5B55_13350) coaE 2760627..2761241 (+) 615 WP_009241592.1 dephospho-CoA kinase -
  N5B55_RS13350 (N5B55_13355) zapD 2761377..2762135 (+) 759 WP_009241593.1 cell division protein ZapD -
  N5B55_RS13355 (N5B55_13360) - 2762144..2762347 (+) 204 WP_024972078.1 DNA gyrase inhibitor YacG -
  N5B55_RS13360 (N5B55_13365) - 2762340..2762801 (-) 462 WP_369812407.1 NUDIX domain-containing protein -
  N5B55_RS13365 (N5B55_13370) - 2762779..2763663 (-) 885 WP_009241596.1 ATP-binding protein -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 46634.86 Da        Isoelectric Point: 10.3593

>NTDB_id=729262 N5B55_RS13335 WP_304538419.1 2758470..2759732(+) (pilG) [Ralstonia pickettii strain MCR]
MATRAPAANRAAAPARKTAKTKAPTQYIFEWEGKDRKGKIFKGEMRAESITEVNAILRKQGLSITKSKRRRAARGKKITP
KDIAYFTRQLSTMLKAGVPLLQSIDIIAKGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDTLYCNLIDAGEQGGI
LDALLERLSLYMEKSIALKAQIKSAMIYPISVLTVAFLVTVVLMVFVVPSFKSVFSSFGATLPAPTLFVMGISDFFVQWW
YIIVFAPIVAITLFVRAFKRSENVQRSTHRWILRIPIFGDIIRKATIARWTRTLATMFAAGTPLVESMESVAGAAGNWTY
HDATLEIQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDEAVANISTLMEPIIIVILGV
LIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=729262 N5B55_RS13335 WP_304538419.1 2758470..2759732(+) (pilG) [Ralstonia pickettii strain MCR]
ATGGCCACACGAGCACCTGCCGCAAACCGCGCTGCCGCGCCGGCACGAAAGACCGCCAAGACCAAGGCGCCCACGCAGTA
CATCTTCGAGTGGGAAGGCAAAGATCGGAAAGGCAAGATCTTCAAGGGCGAGATGCGCGCCGAGAGCATCACCGAGGTGA
ATGCCATCCTGCGCAAGCAAGGCCTGTCGATCACCAAATCCAAGCGCCGCCGCGCGGCCCGCGGCAAGAAGATCACGCCG
AAGGACATCGCCTACTTCACGCGCCAGCTGTCGACCATGCTGAAGGCCGGCGTGCCGCTGCTGCAGTCGATCGACATCAT
CGCAAAGGGCCACGCCAACCCGAACTTCACGCAGCTGCTGACGGAAATCCGCGTCGATATCGAATCGGGCAGCAGCATGG
CGCAAGCCTTCCGTCGTCACCCGAAGTACTTCGACACGCTGTATTGCAACCTGATCGACGCGGGCGAACAGGGCGGTATT
CTGGACGCCCTGCTCGAGCGGCTTTCGCTTTACATGGAAAAATCGATCGCGCTGAAGGCGCAGATCAAGTCGGCGATGAT
TTACCCGATTTCGGTGCTGACGGTGGCCTTCCTTGTGACGGTGGTGCTGATGGTATTCGTAGTGCCCTCGTTCAAAAGCG
TGTTTTCAAGCTTTGGCGCCACGCTTCCCGCTCCGACGTTGTTCGTGATGGGCATCTCCGACTTCTTCGTGCAGTGGTGG
TACATCATTGTCTTCGCGCCCATCGTTGCCATTACGCTCTTCGTCCGGGCGTTCAAGCGCTCGGAAAACGTCCAGCGCTC
CACGCATCGATGGATTTTACGGATTCCTATCTTTGGTGACATTATCCGCAAGGCAACGATTGCGCGTTGGACGCGCACGC
TGGCGACAATGTTCGCCGCGGGCACACCGCTGGTGGAATCGATGGAATCCGTCGCGGGTGCCGCAGGCAACTGGACCTAC
CACGATGCCACCTTGGAAATTCAGCAAGCCGTGCGTATCGGTACCAGCCTGACAAACGCCATGCAGGCCACGCACGTGTT
CGACAACATGGTGCTGCAGATGACGCAGATCGGCGAGGAATCCGGCGCGTTGGACAACATGCTGTTGAAGGTGGCCGAGT
TTTACGAGCGCGAAGTGGATGAAGCGGTGGCGAACATTTCTACGCTGATGGAGCCGATCATCATTGTGATTCTGGGCGTG
CTGATCGGTGGCATGGTCGTTGCGATGTATTTGCCGATCTTCAAGCTGGGTCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(86-209)

Type II secretion system (T2SS), protein F (T2SSF)

(290-412)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

51.128

95

0.486
  pilG Neisseria meningitidis 44/76-A

50.877

95

0.483
  pilC Pseudomonas stutzeri DSM 10701

50.249

95.714

0.481
  pilC Acinetobacter baylyi ADP1

47.912

96.905

0.464
  pilC Acinetobacter baumannii D1279779

47.42

96.905

0.46
  pilC Legionella pneumophila strain ERS1305867

47.146

95.952

0.452
  pilC Vibrio cholerae strain A1552

40.554

94.524

0.383
  pilC Vibrio campbellii strain DS40M4

39.259

96.429

0.379