Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N5B55_RS13330 Genome accession   NZ_CP104381
Coordinates   2756698..2758425 (+) Length   575 a.a.
NCBI ID   WP_154208977.1    Uniprot ID   -
Organism   Ralstonia pickettii strain MCR     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2751698..2763425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5B55_RS13300 (N5B55_13305) obgE 2751706..2752800 (-) 1095 WP_009241586.1 GTPase ObgE -
  N5B55_RS13305 (N5B55_13310) rpmA 2752998..2753258 (-) 261 WP_009241587.1 50S ribosomal protein L27 -
  N5B55_RS13310 (N5B55_13315) rplU 2753296..2753607 (-) 312 WP_004633023.1 50S ribosomal protein L21 -
  N5B55_RS13315 (N5B55_13320) ispB 2753943..2754872 (+) 930 WP_049779105.1 octaprenyl diphosphate synthase -
  N5B55_RS13325 (N5B55_13330) - 2755165..2756439 (+) 1275 WP_178961058.1 HlyC/CorC family transporter -
  N5B55_RS13330 (N5B55_13335) pilB 2756698..2758425 (+) 1728 WP_154208977.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5B55_RS13335 (N5B55_13340) pilG 2758470..2759732 (+) 1263 WP_304538419.1 type II secretion system F family protein Machinery gene
  N5B55_RS13340 (N5B55_13345) pilD 2759732..2760613 (+) 882 WP_304538420.1 A24 family peptidase Machinery gene
  N5B55_RS13345 (N5B55_13350) coaE 2760627..2761241 (+) 615 WP_009241592.1 dephospho-CoA kinase -
  N5B55_RS13350 (N5B55_13355) zapD 2761377..2762135 (+) 759 WP_009241593.1 cell division protein ZapD -
  N5B55_RS13355 (N5B55_13360) - 2762144..2762347 (+) 204 WP_024972078.1 DNA gyrase inhibitor YacG -
  N5B55_RS13360 (N5B55_13365) - 2762340..2762801 (-) 462 WP_369812407.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63682.51 Da        Isoelectric Point: 7.1547

>NTDB_id=729261 N5B55_RS13330 WP_154208977.1 2756698..2758425(+) (pilB) [Ralstonia pickettii strain MCR]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVGSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSANKHF
AQLRLLPLGRRENRLILATSDPSDPKPIEELRQKMNVAIETVVVEHDKLVRQLSLANEAPAEIKSLFPKQEATQIEYDPG
AAAATRRTTPDGIDDAPVVRFLQKLFTEALLRGASDLHFEPFETFYRVRFRIDGILAQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDRKDKDNKETIERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCKECKKPGPLPKETL
LDAGFTDADLDGSWQPYHPVGCEICNGSGYKGRVGIYQVMPITEAIQEIILTHGTALQIAEQARKEGVLSLRESGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=729261 N5B55_RS13330 WP_154208977.1 2756698..2758425(+) (pilB) [Ralstonia pickettii strain MCR]
ATGACACTCGGACTCGCTCTAGCGCAGAGCCGGCGGATCGCGCCGACGCTACTCACTCAGCTCGAACAGAGCGCGCGTGA
AAAGCGCACGCAACTGATCGACGAACTGGTGGGCAGCGGGATCATGAGTGCGCACGACCTTGCCGTGTTCGTTGCCGGCA
AGTATCAGATGCCGCTGCTCGACCTTGCGCAATACAAGCTCGACACTGTGCCGGCCGTGCTGTCAGCCAACAAGCACTTT
GCCCAGTTGCGCCTGTTGCCGCTGGGCCGCCGCGAAAACCGCCTCATCCTCGCCACGTCAGACCCGAGCGATCCCAAGCC
GATCGAAGAGCTGCGTCAGAAGATGAACGTGGCCATTGAAACGGTCGTCGTCGAGCACGACAAGCTGGTGCGCCAACTGA
GCCTCGCCAACGAGGCGCCGGCCGAGATCAAGTCGCTCTTCCCCAAGCAGGAAGCCACGCAGATCGAGTACGACCCGGGC
GCCGCTGCTGCTACCCGTCGTACCACGCCCGACGGCATCGACGATGCCCCCGTGGTGCGCTTCCTGCAGAAGCTCTTTAC
CGAAGCCCTGTTGCGCGGCGCGTCGGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGTATTGACG
GCATCCTCGCGCAGGTCGCGCAACCGCCGCTGGACATCCGCGACAAGATCGCGACCCGCATCAAGGTGCTGTCGCGCCTC
GACATCTCGGAAAAGCGTGTGCCGCAAGACGGCCGCATGAAGCTGCTGATCACTGTCCCGAAAGACCGCAAGGACAAGGA
CAATAAAGAGACCATCGAGCGCGCCATCGACTTCCGTGTATCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCGTCAACCGAGCGGCTCGATATCGACCAGCTCGGCTACGAGCCGGAGCAGAAGCAGCTGCTGCTCGAC
GTGATCAAGCGCCCGTACGGGATGGTGCTGGTGACCGGCCCGACGGGCTCAGGCAAAACGGTGTCGCTGTACACCTTCCT
GAACCGGCTGAACCAGGGCGACATCAATATCTCGACCGCCGAAGACCCCGCTGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTGAACGACAAGGCGGGGCTGACGTTTGCGGCAGCGCTGAAGTCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGGGATCTGGAAACGGCCGATATCTCGATCAAGGCCGCGCAGACCGGTCACTTGGTGTTTTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACCCGCTTGATGAACATGGGGGTTGCGCCATTCAACATCGCGTCGTCGGTGC
TCTTGATCACGGCACAGCGGTTGGCGCGACGGCTGTGCAAGGAATGCAAGAAGCCCGGTCCGCTGCCCAAGGAAACGCTG
CTCGATGCAGGCTTTACCGACGCCGATCTAGACGGCTCATGGCAGCCGTATCACCCGGTCGGCTGCGAGATCTGCAACGG
CAGCGGCTACAAGGGACGGGTCGGCATCTACCAGGTCATGCCGATTACGGAAGCGATCCAGGAAATCATTCTCACACACG
GTACGGCGCTGCAGATCGCCGAGCAAGCGCGCAAGGAAGGCGTGCTGTCGCTGCGCGAATCCGGCTTGCGAAAAGTGAAG
CAGGGGCTGACTTCCCTAGAGGAAGTGCTGGCCACAACGAACCAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.681

98.261

0.508
  pilF Neisseria gonorrhoeae MS11

53.704

93.913

0.504
  pilB Acinetobacter baylyi ADP1

50.709

98.087

0.497
  pilB Legionella pneumophila strain ERS1305867

46.935

99.304

0.466
  pilB Vibrio cholerae strain A1552

45.487

98.261

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.704

95.13

0.435
  pilB Vibrio campbellii strain DS40M4

45.438

95.304

0.433
  pilF Thermus thermophilus HB27

38.393

97.391

0.374