Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NY669_RS01800 Genome accession   NZ_CP103872
Coordinates   412165..413304 (-) Length   379 a.a.
NCBI ID   WP_259433943.1    Uniprot ID   -
Organism   Herbaspirillum huttiense strain ZXN111     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 407165..418304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NY669_RS01770 (NY669_01770) - 407712..408137 (+) 426 WP_039790450.1 NUDIX domain-containing protein -
  NY669_RS01775 (NY669_01775) yacG 408180..408365 (-) 186 WP_034336666.1 DNA gyrase inhibitor YacG -
  NY669_RS01780 (NY669_01780) zapD 408402..409157 (-) 756 WP_034336663.1 cell division protein ZapD -
  NY669_RS01785 (NY669_01785) coaE 409371..409991 (-) 621 WP_034336660.1 dephospho-CoA kinase -
  NY669_RS01790 (NY669_01790) - 409996..410859 (-) 864 WP_259433941.1 A24 family peptidase -
  NY669_RS01795 (NY669_01795) - 410913..412178 (-) 1266 WP_259433942.1 type II secretion system F family protein -
  NY669_RS01800 (NY669_01800) pilF 412165..413304 (-) 1140 WP_259433943.1 GspE/PulE family protein Machinery gene
  NY669_RS01805 (NY669_01805) - 413454..414746 (-) 1293 WP_259433944.1 HlyC/CorC family transporter -
  NY669_RS01810 (NY669_01810) - 414920..415888 (-) 969 WP_259433945.1 glyoxylate/hydroxypyruvate reductase A -
  NY669_RS01815 (NY669_01815) - 415885..416742 (-) 858 WP_039790457.1 IclR family transcriptional regulator -
  NY669_RS01820 (NY669_01820) - 417009..418094 (+) 1086 WP_034336645.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 40804.48 Da        Isoelectric Point: 8.2654

>NTDB_id=726292 NY669_RS01800 WP_259433943.1 412165..413304(-) (pilF) [Herbaspirillum huttiense strain ZXN111]
MLHTSDTAVADDDVPVVRLLHKLLADAVGMGASDLHFEPFEHLFRVRLRLDGVLHELAQPPLSLRDKLATRLKILARLDI
AEKRLPQDGKMRLALGTRTVDFRVSTLPTQFGEKIVLRILDTAQAALSISDLGYEPAQQEALLQAIGKPHGLVLMTGPTG
SGKTVSLYACLQRLNQPGVNIATAEDPVEINLPGINQVSINERAGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKA
SQTGHLVLSTLHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLCRCRRPAGGTPATFVPHGCPACNQTGFRGRT
GIYQVMPVSAAMQALILAQAGTLELGRQAQAEGIIDLRRAGLLKVLRGETSMGEILACT

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=726292 NY669_RS01800 WP_259433943.1 412165..413304(-) (pilF) [Herbaspirillum huttiense strain ZXN111]
ATGCTGCACACTTCCGACACGGCCGTGGCCGATGATGACGTTCCCGTGGTGCGCCTGCTGCACAAGCTGCTGGCCGATGC
GGTCGGCATGGGCGCTTCCGACCTGCATTTCGAACCCTTTGAACATCTCTTCCGCGTACGGCTGCGGCTGGATGGCGTGC
TGCATGAACTGGCGCAGCCGCCGTTGAGCTTGCGCGACAAGCTGGCCACCCGCCTGAAGATCCTGGCGCGGCTGGATATC
GCCGAAAAGCGCCTGCCCCAGGACGGCAAGATGCGCCTGGCGCTAGGCACGCGCACGGTGGATTTCCGCGTCTCGACGCT
GCCCACGCAGTTCGGTGAAAAGATCGTGCTGCGCATCCTCGACACCGCCCAGGCGGCGTTGTCGATCAGCGACCTCGGCT
ACGAACCGGCCCAGCAGGAAGCGCTGCTGCAGGCCATCGGCAAGCCACACGGGCTGGTCCTGATGACCGGGCCGACCGGC
TCCGGCAAGACGGTCTCGCTGTATGCCTGCCTGCAGCGCCTGAACCAGCCGGGCGTGAACATCGCCACCGCCGAGGACCC
GGTGGAAATCAACCTGCCCGGCATCAATCAGGTCAGCATCAACGAACGCGCCGGACTGGACTTCGCCCTGGCACTGCGCG
CCTTCCTGCGCCAGGATCCGGATGTACTGATGGTGGGCGAGATCCGCGACCTGGAGACCGCCGACATCGCCGTCAAGGCT
TCGCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACGATGCCCCGGCCACGCTCACGCGGCTGCTCAACATGGG
CGTGCCGGCCTACAACATTGCGGCATCGGTCAGCCTGATCGTGGCCCAGCGGCTGGTGCGCAAGCTGTGCCGCTGCCGCC
GTCCGGCCGGTGGCACGCCGGCTACCTTCGTGCCGCACGGCTGCCCGGCCTGCAACCAGACCGGCTTTCGCGGGCGCACC
GGCATTTATCAGGTGATGCCGGTCAGCGCGGCCATGCAAGCGCTGATCCTGGCCCAGGCCGGCACATTGGAACTGGGCCG
GCAGGCGCAAGCGGAGGGCATCATCGACCTGCGGCGGGCGGGGCTGCTGAAGGTCTTGCGCGGTGAAACCAGCATGGGAG
AAATCCTTGCCTGTACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(23-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.298

100

0.578
  pilB Acinetobacter baylyi ADP1

55.325

100

0.562
  pilB Acinetobacter baumannii D1279779

54.381

100

0.557
  pilB Vibrio campbellii strain DS40M4

54.974

100

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

54.45

100

0.549
  pilB Vibrio cholerae strain A1552

54.047

100

0.546
  pilB Legionella pneumophila strain ERS1305867

51.282

100

0.528
  pilB Haemophilus influenzae Rd KW20

46.95

99.472

0.467
  pilB Haemophilus influenzae 86-028NP

46.684

99.472

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

42.118

100

0.451
  pilF Thermus thermophilus HB27

43.117

100

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.772

100

0.435
  pilB Glaesserella parasuis strain SC1401

49.057

83.905

0.412
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.633

100

0.398