Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR77_RS03000 Genome accession   NZ_CP103822
Coordinates   660611..661996 (+) Length   461 a.a.
NCBI ID   WP_279441159.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3617     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 655611..666996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR77_RS02975 (NYR77_02970) greB 655681..656169 (-) 489 WP_275216992.1 transcription elongation factor GreB -
  NYR77_RS02980 (NYR77_02975) - 656179..657113 (-) 935 Protein_574 KpsF/GutQ family sugar-phosphate isomerase -
  NYR77_RS02985 (NYR77_02980) pepB 657241..658527 (-) 1287 WP_279441405.1 aminopeptidase PepB -
  NYR77_RS02990 (NYR77_02985) radA 658593..659972 (-) 1380 WP_279441157.1 DNA repair protein RadA Machinery gene
  NYR77_RS02995 (NYR77_02990) pilA 660153..660590 (+) 438 WP_279441158.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR77_RS03000 (NYR77_02995) pilB 660611..661996 (+) 1386 WP_279441159.1 GspE/PulE family protein Machinery gene
  NYR77_RS03005 (NYR77_03000) pilC 661989..663182 (+) 1194 WP_279441160.1 type II secretion system F family protein Machinery gene
  NYR77_RS03010 (NYR77_03005) - 663182..663856 (+) 675 WP_279441161.1 prepilin peptidase -
  NYR77_RS03015 (NYR77_03010) coaE 663878..664516 (+) 639 WP_279441162.1 dephospho-CoA kinase -
  NYR77_RS03020 (NYR77_03015) yacG 664498..664686 (+) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR77_RS03025 (NYR77_03020) holA 664763..665788 (-) 1026 WP_279441163.1 DNA polymerase III subunit delta -
  NYR77_RS03030 (NYR77_03025) lptE 665896..666390 (-) 495 WP_275216984.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52558.19 Da        Isoelectric Point: 5.4993

>NTDB_id=725570 NYR77_RS03000 WP_279441159.1 660611..661996(+) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3617]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSLDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLTALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDRESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGVKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLTKQGKFFERSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725570 NYR77_RS03000 WP_279441159.1 660611..661996(+) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3617]
ATGCAGTATTCGGTTTGTGATATGAATAGTCAGCAAGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCTGGATAA
TTTAGAGCTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGTTTAAAACAGAATGCTT
CAGATATTCACTTTGAACCGCAAAAGGAAAAGTTGCTGATTAGATTACGTATTGATGGGGTATTGCACATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAGCAATTATTACTTACAGCGTTACACCAGCCACAAGGGTTGATTCTGGTTACGGGGCCAACAGGCAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACTGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTAAATCGTGCAATTCAATTAGACTTTAGCCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCGGGAAAGTGCCGAAATTGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAACGCTTATTACAATTGGGAGTTAAAGAGTACG
AAATTAAGAATTCTCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGATGT
GATAACTGTTATCACGGTTATAAAGGGCGAACGGGAATTTATCAGTTATTGACGAAGCAAGGTAAATTTTTTGAAAGATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.489

99.349

0.72
  pilB Haemophilus influenzae Rd KW20

56.984

97.831

0.557
  pilB Haemophilus influenzae 86-028NP

56.319

97.831

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

41.788

100

0.436
  pilB Vibrio campbellii strain DS40M4

40.412

100

0.425
  pilB Legionella pneumophila strain ERS1305867

39.837

100

0.425
  pilB Vibrio cholerae strain A1552

39.357

100

0.425
  pilB Acinetobacter baylyi ADP1

37.972

100

0.414
  pilB Acinetobacter baumannii D1279779

38.306

100

0.412
  pilF Neisseria gonorrhoeae MS11

38.967

92.408

0.36