Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR77_RS02995 Genome accession   NZ_CP103822
Coordinates   660153..660590 (+) Length   145 a.a.
NCBI ID   WP_279441158.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3617     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 655153..665590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR77_RS02975 (NYR77_02970) greB 655681..656169 (-) 489 WP_275216992.1 transcription elongation factor GreB -
  NYR77_RS02980 (NYR77_02975) - 656179..657113 (-) 935 Protein_574 KpsF/GutQ family sugar-phosphate isomerase -
  NYR77_RS02985 (NYR77_02980) pepB 657241..658527 (-) 1287 WP_279441405.1 aminopeptidase PepB -
  NYR77_RS02990 (NYR77_02985) radA 658593..659972 (-) 1380 WP_279441157.1 DNA repair protein RadA Machinery gene
  NYR77_RS02995 (NYR77_02990) pilA 660153..660590 (+) 438 WP_279441158.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR77_RS03000 (NYR77_02995) pilB 660611..661996 (+) 1386 WP_279441159.1 GspE/PulE family protein Machinery gene
  NYR77_RS03005 (NYR77_03000) pilC 661989..663182 (+) 1194 WP_279441160.1 type II secretion system F family protein Machinery gene
  NYR77_RS03010 (NYR77_03005) - 663182..663856 (+) 675 WP_279441161.1 prepilin peptidase -
  NYR77_RS03015 (NYR77_03010) coaE 663878..664516 (+) 639 WP_279441162.1 dephospho-CoA kinase -
  NYR77_RS03020 (NYR77_03015) yacG 664498..664686 (+) 189 WP_005623139.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15603.92 Da        Isoelectric Point: 7.5775

>NTDB_id=725569 NYR77_RS02995 WP_279441158.1 660153..660590(+) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3617]
MKFVFKPIQQAFTLIELMIVIAIIAILTTVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGEVKNCSSGTNGIKN
GNDIKNSKYLDSISVASGVITVKGKGSVDGYSYTLTPDYSNKTLTWETACLGEDTSLFPAAFCSK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725569 NYR77_RS02995 WP_279441158.1 660153..660590(+) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3617]
ATGAAATTCGTATTCAAGCCTATACAGCAGGCATTTACCCTGATTGAATTAATGATTGTAATTGCAATCATTGCTATTTT
GACAACGGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCGGCATTATCCGAACTGTTAGCGGCATCTTCCGCTT
ATAAAACAGATGTGGAAATTTGTATTTATAACACGGGAGAGGTAAAAAACTGTTCTAGCGGAACGAATGGCATCAAAAAT
GGTAACGATATCAAAAACTCTAAGTATTTAGATTCTATTTCTGTAGCTTCCGGTGTAATAACGGTGAAGGGGAAAGGGAG
TGTAGATGGGTATAGCTATACGCTTACACCAGATTATTCAAATAAAACGCTAACTTGGGAGACAGCTTGTTTAGGCGAGG
ATACTAGTTTATTCCCTGCCGCGTTCTGTTCGAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

64.138

100

0.641
  pilA Haemophilus influenzae Rd KW20

55.319

97.241

0.538
  pilA Haemophilus influenzae 86-028NP

55.714

96.552

0.538