Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M52SOB_RS06855 Genome accession   NZ_AP019373
Coordinates   1374224..1375315 (-) Length   363 a.a.
NCBI ID   WP_131111170.1    Uniprot ID   -
Organism   Sulfuricystis thermophila strain M52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1369224..1380315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M52SOB_RS06825 thpR 1369942..1370514 (-) 573 WP_131111166.1 RNA 2',3'-cyclic phosphodiesterase -
  M52SOB_RS06830 ispF 1370507..1370989 (-) 483 WP_284155238.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  M52SOB_RS06835 ispD 1370986..1371672 (-) 687 WP_131111167.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  M52SOB_RS06840 amrB 1371762..1372580 (+) 819 WP_284155016.1 AmmeMemoRadiSam system protein B -
  M52SOB_RS06845 amrA 1372573..1373130 (+) 558 WP_131111168.1 AmmeMemoRadiSam system protein A -
  M52SOB_RS06850 amrS 1373106..1374200 (+) 1095 WP_131111169.1 AmmeMemoRadiSam system radical SAM enzyme -
  M52SOB_RS06855 pilU 1374224..1375315 (-) 1092 WP_131111170.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M52SOB_RS06860 - 1375408..1376157 (+) 750 WP_131111171.1 fused MFS/spermidine synthase -
  M52SOB_RS06865 - 1376178..1376525 (+) 348 WP_172601771.1 ArsC family reductase -
  M52SOB_RS06870 - 1376527..1377363 (+) 837 WP_131111172.1 Hsp33 family molecular chaperone HslO -
  M52SOB_RS06875 gcvT 1377689..1378780 (+) 1092 WP_131111173.1 glycine cleavage system aminomethyltransferase GcvT -
  M52SOB_RS06880 gcvH 1378785..1379165 (+) 381 WP_284155017.1 glycine cleavage system protein GcvH -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 40032.31 Da        Isoelectric Point: 8.1588

>NTDB_id=72502 M52SOB_RS06855 WP_131111170.1 1374224..1375315(-) (pilU) [Sulfuricystis thermophila strain M52]
MIEIQELLHQMLERGASDLFISAEIPPHLKIQGRTLPAQRAGRSWPALAPGESRRFAYALMNEAQRAEFEKNKECNFALA
TRGGGRYRVNVYYQRGEVSMVIRLIKTNVPSFEELGLPPQAGQLALLRRGLVLVVGAAGMGKSTTLAAMVAHRARTLDGH
ILSVEDPIEYIYPHGKSVVEQREVGIDTLSFADALKNMMRQAPDVIVIGEIRDRETAKNAIMYAEAGALCLSTMHSNNAN
QAIERIVNFFPEELHRQVLLDLSLNLKGVISQRLLPGLHGGQVLATEVLLQSAYSSSLIQKGEVAQLKEAIRKGTASGMQ
LFDDCLLELCRARRIAPEEALNHADSKTDLALKLRLAVAPERL

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=72502 M52SOB_RS06855 WP_131111170.1 1374224..1375315(-) (pilU) [Sulfuricystis thermophila strain M52]
ATGATCGAAATCCAGGAACTGTTGCACCAGATGCTCGAGCGCGGGGCCTCCGACCTGTTCATTTCTGCGGAGATTCCGCC
CCATTTGAAGATTCAAGGTCGAACACTTCCGGCGCAGCGGGCAGGCAGATCATGGCCTGCCCTGGCGCCTGGCGAATCAC
GTCGCTTCGCGTATGCCTTGATGAATGAAGCGCAGCGCGCCGAATTCGAAAAGAACAAGGAATGCAATTTCGCACTCGCC
ACGCGTGGGGGCGGACGTTATCGCGTCAACGTCTATTACCAGCGCGGTGAGGTGTCGATGGTGATCCGCCTGATCAAGAC
GAACGTTCCCTCGTTTGAAGAGTTGGGTCTGCCGCCCCAGGCTGGGCAGCTCGCGCTGTTGCGACGTGGCCTCGTGCTGG
TGGTCGGTGCGGCGGGCATGGGCAAGAGTACGACGCTGGCAGCCATGGTTGCACACCGGGCGCGGACGCTGGACGGCCAC
ATCCTTTCGGTCGAAGACCCGATCGAGTACATCTATCCACACGGCAAGTCGGTCGTCGAGCAGCGCGAAGTGGGCATCGA
TACCTTGAGCTTTGCCGATGCGCTCAAGAACATGATGCGCCAGGCCCCGGACGTCATTGTCATCGGCGAGATCCGCGATC
GCGAGACGGCCAAAAACGCCATCATGTATGCCGAAGCCGGTGCCTTGTGTCTGTCGACGATGCATTCGAACAATGCCAAC
CAGGCGATCGAGCGCATCGTCAATTTCTTTCCCGAGGAGTTGCACCGTCAGGTGCTGCTCGATCTGTCGCTCAACCTCAA
AGGTGTCATCTCCCAAAGGCTCCTGCCCGGATTGCATGGCGGCCAGGTGTTGGCGACCGAGGTGTTGTTGCAGTCGGCCT
ATTCGTCATCGCTGATCCAGAAAGGCGAGGTCGCTCAGCTCAAGGAAGCCATTCGCAAGGGAACCGCCAGCGGCATGCAA
TTGTTCGACGACTGTCTGCTGGAACTTTGCCGTGCCAGGCGCATCGCGCCCGAAGAGGCGCTCAATCACGCCGATTCGAA
GACCGATCTCGCGCTGAAGCTGCGGCTGGCCGTCGCTCCCGAACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.42

98.347

0.496
  pilU Vibrio cholerae strain A1552

47.606

97.796

0.466
  pilU Acinetobacter baylyi ADP1

44.568

98.898

0.441
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.88

96.694

0.405
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

38.636

96.97

0.375
  pilT Vibrio cholerae strain A1552

38.636

96.97

0.375
  pilT Legionella pneumophila strain Lp02

38.905

95.592

0.372
  pilT Legionella pneumophila strain ERS1305867

38.905

95.592

0.372
  pilT Pseudomonas aeruginosa PAK

39.528

93.388

0.369

Multiple sequence alignment