Detailed information NTG0723596

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: PWP91_RS12900
• Genome accession: NZ_CP118738
• Coordinates: 2542047..2542985 (-)
• Length: 312 a.a.
• NCBI ID: WP_033900707.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain P-1
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2537047..2547985

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWP91_RS12865 (PWP91_12865)	-	2537210..2537416 (-)	207	WP_046782288.1	hypothetical protein	-
PWP91_RS12870 (PWP91_12870)	-	2537666..2538517 (+)	852	WP_274998651.1	helix-turn-helix domain-containing protein	-
PWP91_RS12875 (PWP91_12875)	-	2538543..2538740 (+)	198	WP_046124795.1	hypothetical protein	-
PWP91_RS12880 (PWP91_12880)	-	2538800..2539351 (+)	552	WP_274998652.1	hypothetical protein	-
PWP91_RS12885 (PWP91_12885)	-	2539472..2540929 (+)	1458	WP_274998653.1	recombinase family protein	-
PWP91_RS12890 (PWP91_12890)	-	2540926..2541066 (-)	141	WP_247649129.1	hypothetical protein	-
PWP91_RS12895 (PWP91_12895)	comGB	2541080..2542153 (-)	1074	WP_274998995.1	competence type IV pilus assembly protein ComGB	Machinery gene
PWP91_RS12900 (PWP91_12900)	comGA	2542047..2542985 (-)	939	WP_033900707.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 312 a.a.
• Molecular weight: 35691.92 Da
• Isoelectric Point: 6.1811

>NTDB_id=723596 PWP91_RS12900 WP_033900707.1 2542047..2542985(-) (comGA) [Lactococcus lactis strain P-1]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide

• Length: 939 bp

>NTDB_id=723596 PWP91_RS12900 WP_033900707.1 2542047..2542985(-) (comGA) [Lactococcus lactis strain P-1]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (9-265)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Lactococcus lactis subsp. cremoris KW2	77.244	100	0.772
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	54.984	99.679	0.548
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	53.376	99.679	0.532
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	52.412	99.679	0.522
comGA/cglA/cilD	Streptococcus pneumoniae D39	52.412	99.679	0.522
comGA/cglA/cilD	Streptococcus pneumoniae R6	52.412	99.679	0.522
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	52.412	99.679	0.522
comGA	Streptococcus mutans UA140	52.244	100	0.522
comGA	Streptococcus mutans UA159	52.244	100	0.522
comGA	Streptococcus gordonii str. Challis substr. CH1	51.757	100	0.519