Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   PWP91_RS12895 Genome accession   NZ_CP118738
Coordinates   2541080..2542153 (-) Length   357 a.a.
NCBI ID   WP_274998995.1    Uniprot ID   -
Organism   Lactococcus lactis strain P-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2536080..2547153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWP91_RS12855 (PWP91_12855) - 2536172..2536411 (-) 240 WP_274998649.1 helix-turn-helix domain-containing protein -
  PWP91_RS12860 (PWP91_12860) - 2536567..2537238 (+) 672 WP_274998650.1 DUF4145 domain-containing protein -
  PWP91_RS12865 (PWP91_12865) - 2537210..2537416 (-) 207 WP_046782288.1 hypothetical protein -
  PWP91_RS12870 (PWP91_12870) - 2537666..2538517 (+) 852 WP_274998651.1 helix-turn-helix domain-containing protein -
  PWP91_RS12875 (PWP91_12875) - 2538543..2538740 (+) 198 WP_046124795.1 hypothetical protein -
  PWP91_RS12880 (PWP91_12880) - 2538800..2539351 (+) 552 WP_274998652.1 hypothetical protein -
  PWP91_RS12885 (PWP91_12885) - 2539472..2540929 (+) 1458 WP_274998653.1 recombinase family protein -
  PWP91_RS12890 (PWP91_12890) - 2540926..2541066 (-) 141 WP_247649129.1 hypothetical protein -
  PWP91_RS12895 (PWP91_12895) comGB 2541080..2542153 (-) 1074 WP_274998995.1 competence type IV pilus assembly protein ComGB Machinery gene
  PWP91_RS12900 (PWP91_12900) comGA 2542047..2542985 (-) 939 WP_033900707.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40224.43 Da        Isoelectric Point: 10.3543

>NTDB_id=723595 PWP91_RS12895 WP_274998995.1 2541080..2542153(-) (comGB) [Lactococcus lactis strain P-1]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLLKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFGQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=723595 PWP91_RS12895 WP_274998995.1 2541080..2542153(-) (comGB) [Lactococcus lactis strain P-1]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTAAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGGACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

82.991

95.518

0.793
  comGB Streptococcus gordonii str. Challis substr. CH1

53.666

95.518

0.513
  comGB Streptococcus mutans UA140

52.647

95.238

0.501
  comGB Streptococcus mutans UA159

52.353

95.238

0.499
  comGB/cglB Streptococcus mitis SK321

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae D39

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae R6

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

93.838

0.473