Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EZU31_RS03455 Genome accession   NZ_AP019193
Coordinates   719675..720784 (-) Length   369 a.a.
NCBI ID   WP_017411211.1    Uniprot ID   A0A1C2L453
Organism   Aeromonas hydrophila strain GSH8-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 714675..725784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZU31_RS03435 (AHGSH82_006470) brnQ 715132..716439 (-) 1308 WP_011707394.1 branched-chain amino acid transport system II carrier protein -
  EZU31_RS03440 (AHGSH82_006480) - 716631..717341 (-) 711 WP_130631668.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  EZU31_RS03445 (AHGSH82_006490) srmB 717470..718693 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  EZU31_RS03450 (AHGSH82_006500) yaaA 718870..719643 (-) 774 WP_130631669.1 peroxide stress protein YaaA -
  EZU31_RS03455 (AHGSH82_006510) pilU 719675..720784 (-) 1110 WP_017411211.1 type IVa pilus ATPase TapU Machinery gene
  EZU31_RS03460 (AHGSH82_006520) pilT 720813..721847 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  EZU31_RS03465 (AHGSH82_006530) - 721887..722588 (+) 702 WP_029304817.1 YggS family pyridoxal phosphate-dependent enzyme -
  EZU31_RS03470 (AHGSH82_006540) proC 722647..723471 (+) 825 WP_130631670.1 pyrroline-5-carboxylate reductase -
  EZU31_RS03475 (AHGSH82_006550) - 723512..724063 (+) 552 WP_016351969.1 YggT family protein -
  EZU31_RS03480 (AHGSH82_006560) yggU 724063..724362 (+) 300 WP_130631671.1 DUF167 family protein YggU -
  EZU31_RS03485 (AHGSH82_006570) - 724381..724800 (+) 420 WP_011707384.1 DUF4426 domain-containing protein -
  EZU31_RS03490 (AHGSH82_006580) - 724857..725168 (-) 312 WP_042064670.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41035.17 Da        Isoelectric Point: 6.5035

>NTDB_id=72194 EZU31_RS03455 WP_017411211.1 719675..720784(-) (pilU) [Aeromonas hydrophila strain GSH8-2]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=72194 EZU31_RS03455 WP_017411211.1 719675..720784(-) (pilU) [Aeromonas hydrophila strain GSH8-2]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCATCTGCAGTCGCTGGCAGAAGCTCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTACCTGCACACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCTATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGACTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCAGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAATTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCATTCGACGT
GGCGCTGAAGAGTTCGCTGCGCCAGGCGCCAGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C2L453

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36

Multiple sequence alignment