Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EZU31_RS02275 Genome accession   NZ_AP019193
Coordinates   482362..483603 (-) Length   413 a.a.
NCBI ID   WP_130631562.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain GSH8-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477362..488603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZU31_RS02245 (AHGSH82_004230) - 478203..479387 (+) 1185 WP_130631558.1 tetratricopeptide repeat protein -
  EZU31_RS02250 (AHGSH82_004240) mutT 479396..479809 (+) 414 WP_174843536.1 8-oxo-dGTP diphosphatase MutT -
  EZU31_RS02255 (AHGSH82_004250) yacG 479870..480064 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  EZU31_RS02260 (AHGSH82_004260) zapD 480074..480796 (-) 723 WP_024945431.1 cell division protein ZapD -
  EZU31_RS02265 (AHGSH82_004270) coaE 480834..481448 (-) 615 WP_130631560.1 dephospho-CoA kinase -
  EZU31_RS02270 (AHGSH82_004280) pilD 481467..482339 (-) 873 WP_130631561.1 prepilin peptidase Machinery gene
  EZU31_RS02275 (AHGSH82_004290) pilC 482362..483603 (-) 1242 WP_130631562.1 type II secretion system F family protein Machinery gene
  EZU31_RS02280 (AHGSH82_004300) pilB 483719..485425 (-) 1707 WP_073351950.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  EZU31_RS02285 tapA 485429..485836 (-) 408 WP_130631563.1 type IVa pilus major pilin TapA -
  EZU31_RS02290 (AHGSH82_004310) - 486138..487274 (+) 1137 WP_021141311.1 ISAs1-like element ISAs12 family transposase -
  EZU31_RS02295 (AHGSH82_004320) nadC 487646..488503 (-) 858 WP_130631564.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45392.55 Da        Isoelectric Point: 9.8637

>NTDB_id=72190 EZU31_RS02275 WP_130631562.1 482362..483603(-) (pilC) [Aeromonas hydrophila strain GSH8-2]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGAKIKPMDIAVVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPAMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQFVIGISRFMQNWWYVIFGGIA
LAIFLYVRAWRASQKVKDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=72190 EZU31_RS02275 WP_130631562.1 482362..483603(-) (pilC) [Aeromonas hydrophila strain GSH8-2]
ATGGCAACGCTAACCCAGAAACAGAATGCCCCCAAGAAAGTCTTCGCCTTTCGCTGGAGTGGGGTGAACCGCAAGGGCCA
GAAAGTCTCCGGCGAATTGCAGGCCGACAGCATCAACACGGTCAAGGCAGAGCTGCGCAAACAGGGTGTCAACGTCACCA
AGGTGAGCAAGAAGAGCCAGGGGTTATTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATCGCCGTCGTCTCTCGC
CAGATCACCACCATGCTGTCAGCCGGCGTACCTCTGGTCCAGAGCCTGCAGATCATCGCTCGCAGCCACGAGAAGGCAGC
CATGCGCGAGCTCATGGGGCAGATTGCCGCCGATGTGGAGACCGGCACCCCTATGTCCGAGGCGCTGCGCCGCCATCCCC
GTCATTTTGACGATCTCTACTGCGATCTGGTGGAGGCTGGCGAACAGTCCGGCGCTCTGGAGACCATCTACGATCGCATC
GCCACCTATCGCGAGAAATCGGAAGCCCTCAAGTCCAAGATCAAGAAAGCCATGTTCTACCCCGCCATGGTCATCCTGGT
GGCCATTGTTGTTACCTCCATCCTGCTGCTGTTTGTCATTCCCCAGTTCGAGGACATCTTCAAGAGCTTCGGTGCCGAGC
TGCCCATCTTCACCCAATTCGTCATCGGCATCTCCCGTTTCATGCAGAACTGGTGGTATGTGATTTTTGGCGGCATAGCG
CTCGCCATCTTTCTCTACGTGCGCGCCTGGCGCGCCTCCCAGAAGGTCAAGGACAACACCGACAAGTTCATACTGACCAT
CCCGGTGGTTGGCATGATATTGCACAAGGCCGCTATGGCCCGTTTCGCCCGCACCCTCTCCACCACCTTCTCCGCCGGTA
TTCCACTAGTGGATGCCTTGGTCTCTGCGGCAGGTGCCTCCGGTAACTATGTCTACCGCACTGCCGTCATGGCCATTCGC
AACGAGGTGGTGGCGGGTATGCAGATCAACGTGGCCATGCGCACCGTCGATCTGTTCCCAGACATGGTGATCCAGATGGT
GATGATCGGCGAGGAGTCTGGCGCCATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAGCAGGAGGTGGACGATC
TGGTCGACGGCCTCACCAGCCTGCTGGAACCTCTCATCATGGTGGTGCTGGGGGTTCTGGTCGGCGGCATGGTGGTCGCC
ATGTATCTGCCCATCTTCAAACTGGGATCGGTGATCCACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.323

95.884

0.55
  pilC Acinetobacter baumannii D1279779

54.25

96.852

0.525
  pilC Acinetobacter baylyi ADP1

52.393

96.126

0.504
  pilC Legionella pneumophila strain ERS1305867

51.358

98.063

0.504
  pilC Vibrio cholerae strain A1552

47.739

96.368

0.46
  pilC Vibrio campbellii strain DS40M4

46.599

96.126

0.448
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilG Neisseria meningitidis 44/76-A

40

98.063

0.392
  pilC Thermus thermophilus HB27

37.75

96.852

0.366

Multiple sequence alignment