Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EZU31_RS02280 Genome accession   NZ_AP019193
Coordinates   483719..485425 (-) Length   568 a.a.
NCBI ID   WP_073351950.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain GSH8-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478719..490425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZU31_RS02250 (AHGSH82_004240) mutT 479396..479809 (+) 414 WP_174843536.1 8-oxo-dGTP diphosphatase MutT -
  EZU31_RS02255 (AHGSH82_004250) yacG 479870..480064 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  EZU31_RS02260 (AHGSH82_004260) zapD 480074..480796 (-) 723 WP_024945431.1 cell division protein ZapD -
  EZU31_RS02265 (AHGSH82_004270) coaE 480834..481448 (-) 615 WP_130631560.1 dephospho-CoA kinase -
  EZU31_RS02270 (AHGSH82_004280) pilD 481467..482339 (-) 873 WP_130631561.1 prepilin peptidase Machinery gene
  EZU31_RS02275 (AHGSH82_004290) pilC 482362..483603 (-) 1242 WP_130631562.1 type II secretion system F family protein Machinery gene
  EZU31_RS02280 (AHGSH82_004300) pilB 483719..485425 (-) 1707 WP_073351950.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  EZU31_RS02285 tapA 485429..485836 (-) 408 WP_130631563.1 type IVa pilus major pilin TapA -
  EZU31_RS02290 (AHGSH82_004310) - 486138..487274 (+) 1137 WP_021141311.1 ISAs1-like element ISAs12 family transposase -
  EZU31_RS02295 (AHGSH82_004320) nadC 487646..488503 (-) 858 WP_130631564.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EZU31_RS02300 (AHGSH82_004330) - 488509..488979 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  EZU31_RS02305 (AHGSH82_004340) ampD 489127..489699 (+) 573 WP_130631566.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4363

>NTDB_id=72191 EZU31_RS02280 WP_073351950.1 483719..485425(-) (pilB) [Aeromonas hydrophila strain GSH8-2]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDERQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=72191 EZU31_RS02280 WP_073351950.1 483719..485425(-) (pilB) [Aeromonas hydrophila strain GSH8-2]
ATGACCTCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCTTCTTCCCTGCTCAGTGAGTCCGACTCACAGCGCTA
TCTGAGTCAAGCCAAGGCCCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAGGCACTGG
CCGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGTTGGTCGAGGAGAACA
AACTCACCACTGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAGGTCTCGGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCTGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGTGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGGTAGCAACTCCGCCAGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CCGCACGCCTCAATATCGAGCAACTCGGTTTCGACGAGCGCCAGAAGGCGCAATACCTGCGTGCCCTGTCGAAGCCTCAG
GGCATGATTCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGGCTGACCTTCGCCAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTACACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCAGGTGGGTACAAGGGCAGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGTACCTCTGGCCTTGAGAAGGCTCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.59

99.648

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

51.601

98.944

0.511
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.355

85.211

0.361

Multiple sequence alignment