Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PSH70_RS04020 Genome accession   NZ_CP117438
Coordinates   933402..933815 (+) Length   137 a.a.
NCBI ID   WP_069553305.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FP2327     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 928402..938815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH70_RS03985 (PSH70_03985) - 928881..929348 (+) 468 WP_069553301.1 FAD/FMN-containing dehydrogenase -
  PSH70_RS03990 (PSH70_03990) - 929345..930034 (+) 690 WP_053254336.1 energy-coupling factor ABC transporter permease -
  PSH70_RS03995 (PSH70_03995) - 930099..930314 (+) 216 WP_003171680.1 hypothetical protein -
  PSH70_RS04000 (PSH70_04000) yacG 930311..930511 (-) 201 WP_053254337.1 DNA gyrase inhibitor YacG -
  PSH70_RS04005 (PSH70_04005) coaE 930508..931131 (-) 624 WP_053254338.1 dephospho-CoA kinase -
  PSH70_RS04010 (PSH70_04010) pilD 931128..931994 (-) 867 WP_305439934.1 A24 family peptidase Machinery gene
  PSH70_RS04015 (PSH70_04015) pilC 931991..933199 (-) 1209 WP_053254340.1 type II secretion system F family protein Machinery gene
  PSH70_RS04020 (PSH70_04020) pilA/pilAI 933402..933815 (+) 414 WP_069553305.1 pilin Machinery gene
  PSH70_RS04030 (PSH70_04030) - 934123..934428 (+) 306 WP_053254342.1 DUF6388 family protein -
  PSH70_RS04035 (PSH70_04035) nadC 934475..935323 (-) 849 WP_069553306.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PSH70_RS04040 (PSH70_04040) - 935567..937741 (+) 2175 WP_301279262.1 DUF1631 domain-containing protein -
  PSH70_RS04045 (PSH70_04045) ampD 937785..938348 (+) 564 WP_069553308.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 13890.21 Da        Isoelectric Point: 8.1205

>NTDB_id=718086 PSH70_RS04020 WP_069553305.1 933402..933815(+) (pilA/pilAI) [Pseudomonas fluorescens strain FP2327]
MRQKGFTLIELLIVVAIIGILATIGLPMYTKHQAKAKFTAGLAEVAALKAGFEDTLNQGTVPTLALIGGTSPTANCKIEV
AGDVATGAGSIRCEILDAPAPVLGKTITLTRSATTGWKCATTADAQYVAKGCGADGA

Nucleotide


Download         Length: 414 bp        

>NTDB_id=718086 PSH70_RS04020 WP_069553305.1 933402..933815(+) (pilA/pilAI) [Pseudomonas fluorescens strain FP2327]
ATGCGTCAGAAAGGCTTCACCTTGATCGAGTTGTTGATCGTCGTGGCAATCATCGGCATTTTGGCCACGATCGGTTTGCC
CATGTATACCAAGCACCAGGCCAAGGCCAAGTTTACGGCGGGGTTGGCTGAAGTCGCCGCGTTGAAGGCCGGTTTCGAAG
ACACCCTTAACCAGGGCACCGTGCCAACCCTGGCCTTGATCGGTGGCACCAGCCCCACCGCCAACTGCAAGATCGAGGTG
GCAGGCGATGTGGCCACCGGCGCCGGTTCCATCCGTTGTGAAATTCTCGATGCGCCCGCGCCTGTGCTGGGCAAGACCAT
CACGTTAACGCGCAGTGCCACCACGGGCTGGAAGTGTGCAACCACGGCTGACGCTCAATACGTTGCCAAGGGCTGCGGTG
CCGACGGAGCATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(34-132)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.588

99.27

0.453
  comP Acinetobacter baylyi ADP1

39.597

100

0.431
  pilA Pseudomonas aeruginosa PAK

38.926

100

0.423
  pilA Acinetobacter baumannii strain A118

41.912

99.27

0.416
  pilA Vibrio cholerae C6706

38.194

100

0.401
  pilA Vibrio cholerae strain A1552

38.194

100

0.401
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.194

100

0.401
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.519

98.54

0.38
  pilA Vibrio parahaemolyticus RIMD 2210633

37.594

97.08

0.365