Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NRZ29_RS03300 Genome accession   NZ_CP102370
Coordinates   700436..701545 (-) Length   369 a.a.
NCBI ID   WP_268030052.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BC19     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 695436..706545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ29_RS03280 (NRZ29_03280) brnQ 695893..697182 (-) 1290 WP_187268869.1 branched-chain amino acid transport system II carrier protein -
  NRZ29_RS03285 (NRZ29_03285) - 697392..698102 (-) 711 WP_268030050.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NRZ29_RS03290 (NRZ29_03290) srmB 698231..699454 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  NRZ29_RS03295 (NRZ29_03295) yaaA 699631..700404 (-) 774 WP_268030051.1 peroxide stress protein YaaA -
  NRZ29_RS03300 (NRZ29_03300) pilU 700436..701545 (-) 1110 WP_268030052.1 type IVa pilus ATPase TapU Machinery gene
  NRZ29_RS03305 (NRZ29_03305) pilT 701574..702608 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  NRZ29_RS03310 (NRZ29_03310) - 702648..703349 (+) 702 WP_187268865.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRZ29_RS03315 (NRZ29_03315) proC 703408..704232 (+) 825 WP_268030053.1 pyrroline-5-carboxylate reductase -
  NRZ29_RS03320 (NRZ29_03320) - 704273..704824 (+) 552 WP_017411214.1 YggT family protein -
  NRZ29_RS03325 (NRZ29_03325) yggU 704824..705123 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  NRZ29_RS03330 (NRZ29_03330) - 705142..705561 (+) 420 WP_060390705.1 DUF4426 domain-containing protein -
  NRZ29_RS03335 (NRZ29_03335) - 705618..705929 (-) 312 WP_162498788.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40931.04 Da        Isoelectric Point: 6.3880

>NTDB_id=716578 NRZ29_RS03300 WP_268030052.1 700436..701545(-) (pilU) [Aeromonas hydrophila strain BC19]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLTEVPLDKAGALALVKETLSPEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEVHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=716578 NRZ29_RS03300 WP_268030052.1 700436..701545(-) (pilU) [Aeromonas hydrophila strain BC19]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCGCTGACAGAAGTCCCCCTCGACAAGGCAGGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCCTGAGCACTTCGAGCGCTATCTGCACACCAAGGAGGCCAACTACGCCATTCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATTCCCACCTTTGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGTTATCGCAACCAACATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACTCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAAAGTTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGCGAAATTCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGTCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGATGGGCGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGGTGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.054

99.729

0.599
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363