Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NRL00_RS17960 Genome accession   NZ_CP102327
Coordinates   3949004..3950113 (+) Length   369 a.a.
NCBI ID   WP_010636179.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC05     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3944004..3955113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRL00_RS17925 (NRL00_17925) - 3944611..3944922 (+) 312 WP_005304992.1 hypothetical protein -
  NRL00_RS17930 (NRL00_17930) - 3944986..3945405 (-) 420 WP_005304995.1 DUF4426 domain-containing protein -
  NRL00_RS17935 (NRL00_17935) yggU 3945424..3945723 (-) 300 WP_005304998.1 DUF167 family protein YggU -
  NRL00_RS17940 (NRL00_17940) - 3945723..3946274 (-) 552 WP_017780458.1 YggT family protein -
  NRL00_RS17945 (NRL00_17945) proC 3946315..3947139 (-) 825 WP_099359979.1 pyrroline-5-carboxylate reductase -
  NRL00_RS17950 (NRL00_17950) - 3947200..3947901 (-) 702 WP_201880082.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRL00_RS17955 (NRL00_17955) pilT 3947941..3948975 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  NRL00_RS17960 (NRL00_17960) pilU 3949004..3950113 (+) 1110 WP_010636179.1 type IVa pilus ATPase TapU Machinery gene
  NRL00_RS17965 (NRL00_17965) yaaA 3950145..3950918 (+) 774 WP_017783834.1 peroxide stress protein YaaA -
  NRL00_RS17970 (NRL00_17970) srmB 3951095..3952318 (-) 1224 WP_010636177.1 ATP-dependent RNA helicase SrmB -
  NRL00_RS17975 (NRL00_17975) - 3952447..3953157 (+) 711 WP_226009354.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NRL00_RS17980 (NRL00_17980) brnQ 3953348..3954655 (+) 1308 WP_005305030.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41081.22 Da        Isoelectric Point: 6.5672

>NTDB_id=716172 NRL00_RS17960 WP_010636179.1 3949004..3950113(+) (pilU) [Aeromonas dhakensis strain BC05]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=716172 NRL00_RS17960 WP_010636179.1 3949004..3950113(+) (pilU) [Aeromonas dhakensis strain BC05]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAATGGCCACCTGCAGTCACTGGCAGAAGCGCCCCTCGACAAGGCAAGGGCACTGGCGCTGGTCAAGGAGA
CCCTGAGCCCGGAGCACTTCGAACGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCACTGCCCCCCATATTGCAGGAGATCGCCCTCGCCAAGCGCGGACTGGTGCTGTTCGTCGGCGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAAAGTTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGCGAAATTCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGTCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTGCCGCAGGAGAAACACCGTCAGTTCCTGTTTGATCTCTCCTTCAATCTGCGCGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCATCGGCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAACAGCTGGGTTCAGGCACACTCGACAACGTTACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.87

99.729

0.607
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363