Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NRK99_RS18685 Genome accession   NZ_CP102326
Coordinates   4106269..4107378 (+) Length   369 a.a.
NCBI ID   WP_019838905.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC04     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4101269..4112378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRK99_RS18650 (NRK99_18650) - 4101876..4102187 (+) 312 WP_005304992.1 hypothetical protein -
  NRK99_RS18655 (NRK99_18655) - 4102251..4102670 (-) 420 WP_005304995.1 DUF4426 domain-containing protein -
  NRK99_RS18660 (NRK99_18660) yggU 4102689..4102988 (-) 300 WP_005304998.1 DUF167 family protein YggU -
  NRK99_RS18665 (NRK99_18665) - 4102988..4103539 (-) 552 WP_017780458.1 YggT family protein -
  NRK99_RS18670 (NRK99_18670) proC 4103580..4104404 (-) 825 WP_267999972.1 pyrroline-5-carboxylate reductase -
  NRK99_RS18675 (NRK99_18675) - 4104465..4105166 (-) 702 WP_010636180.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRK99_RS18680 (NRK99_18680) pilT 4105206..4106240 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  NRK99_RS18685 (NRK99_18685) pilU 4106269..4107378 (+) 1110 WP_019838905.1 type IVa pilus ATPase TapU Machinery gene
  NRK99_RS18690 (NRK99_18690) yaaA 4107410..4108183 (+) 774 WP_017783834.1 peroxide stress protein YaaA -
  NRK99_RS18695 (NRK99_18695) srmB 4108360..4109583 (-) 1224 WP_017783071.1 ATP-dependent RNA helicase SrmB -
  NRK99_RS18700 (NRK99_18700) - 4109712..4110422 (+) 711 WP_267999973.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NRK99_RS18705 (NRK99_18705) brnQ 4110613..4111920 (+) 1308 WP_005305030.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41139.26 Da        Isoelectric Point: 6.4437

>NTDB_id=716144 NRK99_RS18685 WP_019838905.1 4106269..4107378(+) (pilU) [Aeromonas dhakensis strain BC04]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=716144 NRK99_RS18685 WP_019838905.1 4106269..4107378(+) (pilU) [Aeromonas dhakensis strain BC04]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCGCCACTCGACAAGGCAAGGGCACTGGCACTGGTGAAGGAGA
CCCTGAGCCCGGAGCACTTCGAACGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTACTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCACTGCCCCCCATACTGCAGGAGATCGCCCTCGCCAAGCGCGGGCTGGTACTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACTCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACGGAGTCGTTCGACGT
GGCGCTGAAGAGTTCGCTGCGTCAAGCGCCGGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGTCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTGCCGCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTGGCCCAGCA
ACTGTTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCATCGCCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAGCAGCTGGGTTCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

61.141

99.729

0.61
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363