Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DLM_RS00420 Genome accession   NZ_AP018823
Coordinates   80030..81250 (+) Length   406 a.a.
NCBI ID   WP_089083787.1    Uniprot ID   A0A3G9G6R3
Organism   Aquitalea magnusonii strain H3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 75030..86250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLM_RS00405 (DLM_0079) - 75284..76675 (-) 1392 WP_089083790.1 cryptochrome/photolyase family protein -
  DLM_RS00410 (DLM_0080) - 77017..78099 (+) 1083 WP_089083789.1 porin -
  DLM_RS00415 (DLM_0081) pilF 78297..80009 (+) 1713 WP_089083788.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DLM_RS00420 (DLM_0082) pilC 80030..81250 (+) 1221 WP_089083787.1 type II secretion system F family protein Machinery gene
  DLM_RS00425 (DLM_0083) - 81253..82121 (+) 869 Protein_84 prepilin peptidase -
  DLM_RS00430 (DLM_0084) coaE 82111..82722 (+) 612 WP_089083785.1 dephospho-CoA kinase -
  DLM_RS00435 (DLM_0085) zapD 82762..83520 (+) 759 WP_089083784.1 cell division protein ZapD -
  DLM_RS00440 (DLM_0086) yacG 83526..83723 (+) 198 WP_089083783.1 DNA gyrase inhibitor YacG -
  DLM_RS00445 (DLM_0087) - 83779..85020 (-) 1242 WP_089083782.1 N-acetylmuramoyl-L-alanine amidase -
  DLM_RS00450 (DLM_0088) tsaE 84978..85466 (-) 489 WP_089083827.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44718.60 Da        Isoelectric Point: 9.9425

>NTDB_id=71531 DLM_RS00420 WP_089083787.1 80030..81250(+) (pilC) [Aquitalea magnusonii strain H3]
MASVAKKVNPGFIWEWEGKDRSGKVIRGELRAETEAVAKTQLRRQGINVVKVKRRRSGFGRKITEKDITLFTRQLSTMMR
AGVPLLQAFDIAAKGHGNPAVTKMLLEVRADVETGLSLAEAFRKRPLYFDKLFCNLIAAGETGGVLDSLLDKLATYKEKV
MAIKGKIKSAMIYPTAIVATAFIITAVIMIYVIPAFKDLFSSFGADLPAPTLVVIWLSDFFVHWWWLIFGSIFGAIFAFF
YAFKRTPKLQEQFDRILLKLPVIGDIIRKATIARWSRTLSTLFAAGVPLVEALDSVGGASGNQVYSEATKLIQNDVSTGS
SLSYSMQRTGLFPNMVLQMTSIGEESGSLDQMLDKIADFYEEEVDNAVASLSSLLEPAIMVILGVLIGGLVIAMYMPIFK
MGQVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=71531 DLM_RS00420 WP_089083787.1 80030..81250(+) (pilC) [Aquitalea magnusonii strain H3]
ATGGCAAGCGTGGCGAAAAAGGTAAACCCTGGGTTTATCTGGGAATGGGAAGGCAAGGACCGATCCGGCAAGGTGATTCG
CGGCGAATTGCGCGCAGAAACCGAAGCGGTTGCCAAAACGCAGTTGCGCCGGCAGGGCATCAATGTCGTCAAGGTGAAAC
GGCGCCGCAGCGGATTCGGTCGCAAGATCACGGAGAAGGATATCACCCTGTTCACGCGTCAGCTCTCCACCATGATGCGT
GCCGGGGTTCCCTTGTTGCAGGCATTTGACATTGCCGCCAAGGGGCATGGCAATCCGGCGGTTACCAAAATGTTGCTGGA
AGTCCGCGCTGATGTGGAAACCGGCCTGTCTTTGGCTGAGGCGTTTCGCAAGCGACCGCTCTATTTCGACAAGCTGTTCT
GTAACCTGATTGCAGCAGGTGAAACCGGTGGTGTGCTGGATTCCCTGCTGGATAAACTGGCCACCTACAAAGAAAAGGTC
ATGGCGATCAAGGGCAAGATCAAGTCCGCCATGATTTACCCTACTGCGATTGTTGCCACGGCCTTCATTATTACCGCCGT
GATCATGATTTACGTGATCCCTGCGTTTAAGGATCTGTTTTCCAGCTTTGGTGCTGATTTGCCGGCCCCGACATTAGTGG
TGATCTGGCTGTCCGACTTTTTTGTGCATTGGTGGTGGCTGATATTTGGCAGCATCTTTGGTGCCATTTTTGCCTTTTTC
TACGCGTTCAAGCGCACACCCAAGCTGCAGGAACAGTTTGACCGCATCCTGCTCAAGCTGCCGGTGATTGGCGATATCAT
CCGCAAGGCAACCATTGCACGCTGGTCGCGTACTTTGTCCACGCTGTTTGCTGCCGGGGTGCCGCTGGTTGAAGCGCTGG
ATTCGGTGGGCGGTGCCTCCGGCAATCAGGTGTACAGCGAGGCCACCAAGCTGATCCAGAATGATGTCAGTACGGGTTCC
AGCCTGAGTTACTCCATGCAGCGTACCGGTCTGTTTCCCAACATGGTGCTGCAGATGACATCCATCGGTGAAGAGTCGGG
CTCACTCGACCAGATGTTGGACAAGATTGCCGACTTCTATGAAGAGGAGGTCGATAATGCGGTTGCATCTTTGTCCAGTT
TGCTCGAGCCGGCCATCATGGTCATCCTTGGCGTGTTGATTGGCGGTCTGGTTATCGCCATGTACATGCCGATTTTCAAG
ATGGGCCAGGTGGTAGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G9G6R3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.58

99.754

0.534
  pilC Legionella pneumophila strain ERS1305867

53.465

99.507

0.532
  pilG Neisseria meningitidis 44/76-A

54.082

96.552

0.522
  pilG Neisseria gonorrhoeae MS11

54.22

96.305

0.522
  pilC Acinetobacter baylyi ADP1

50

100

0.5
  pilC Acinetobacter baumannii D1279779

50.378

97.783

0.493
  pilC Vibrio campbellii strain DS40M4

39.798

97.783

0.389
  pilC Vibrio cholerae strain A1552

37.406

98.768

0.369
  pilC Thermus thermophilus HB27

36.842

98.276

0.362

Multiple sequence alignment