Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DLM_RS00415 Genome accession   NZ_AP018823
Coordinates   78297..80009 (+) Length   570 a.a.
NCBI ID   WP_089083788.1    Uniprot ID   -
Organism   Aquitalea magnusonii strain H3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 73297..85009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLM_RS00395 (DLM_0077) - 73968..74819 (+) 852 WP_231959958.1 cytochrome C assembly family protein -
  DLM_RS00400 (DLM_0078) - 74868..75227 (+) 360 WP_089083791.1 ComEA family DNA-binding protein -
  DLM_RS00405 (DLM_0079) - 75284..76675 (-) 1392 WP_089083790.1 cryptochrome/photolyase family protein -
  DLM_RS00410 (DLM_0080) - 77017..78099 (+) 1083 WP_089083789.1 porin -
  DLM_RS00415 (DLM_0081) pilF 78297..80009 (+) 1713 WP_089083788.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DLM_RS00420 (DLM_0082) pilC 80030..81250 (+) 1221 WP_089083787.1 type II secretion system F family protein Machinery gene
  DLM_RS00425 (DLM_0083) - 81253..82121 (+) 869 Protein_84 prepilin peptidase -
  DLM_RS00430 (DLM_0084) coaE 82111..82722 (+) 612 WP_089083785.1 dephospho-CoA kinase -
  DLM_RS00435 (DLM_0085) zapD 82762..83520 (+) 759 WP_089083784.1 cell division protein ZapD -
  DLM_RS00440 (DLM_0086) yacG 83526..83723 (+) 198 WP_089083783.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62130.50 Da        Isoelectric Point: 5.4209

>NTDB_id=71530 DLM_RS00415 WP_089083788.1 78297..80009(+) (pilF) [Aquitalea magnusonii strain H3]
MEASAGISGLGRALVQNNALLLQDAEAIQRQAQASNISFVEQLIVSKKMSAVDVAAFASRVFGYPLLDMGSIDPTQLPKG
LVDEELIGSRRLVPLFKRGNKLFIGTSDPTQTSAFHAITFKTGLTVEVVVVEDDKLSKVIGKYTEDASAAIKALENEDFG
DLEFVDPDAPTPHSETNQPDVDDAPVVKFIHKVLLDAINGGASDVHFEPYEKFYRIRYRVDGQLKEIAQPPLLLKEKIAS
RIKVISRLDISEKRVPQDGRLKLVLSKSHSIDFRVSTLPTLFGEKIVMRILDSSAASLDIEQLGFEPEQKQMLLDAIGRP
YGMVLVTGPTGSGKTVSLYTCLNILNKPDINISTAEDPVEINLPGINQVNVNEKAGLTFSSALKAFLRQDPDVIMVGEIR
DFETADISIKAAQTGHMVFSTLHTNNAPATLTRLLNMGVAPFNVASSVLLIMAQRLARRLCNHCKAPVDIPEPALLRAGF
RPEELDGSWKPYGPVGCDECRGSGYKGRTGIYEVMPISDAMTRLIMQNGTAIDINDLALKEGMIDLRHAGLLKVKKGLTS
LAEIEAVTND

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=71530 DLM_RS00415 WP_089083788.1 78297..80009(+) (pilF) [Aquitalea magnusonii strain H3]
ATGGAAGCTTCTGCGGGGATATCCGGCCTTGGGCGGGCGCTGGTACAAAACAATGCACTGCTGTTGCAGGATGCCGAGGC
TATTCAGCGTCAAGCACAGGCTTCAAACATCAGTTTCGTTGAGCAGTTGATTGTCAGCAAGAAGATGTCCGCAGTCGATG
TGGCTGCATTTGCTTCCCGAGTGTTTGGCTATCCCTTGCTGGACATGGGGTCTATCGATCCGACGCAGCTACCCAAGGGC
TTGGTGGATGAGGAGCTGATTGGCAGTCGTCGGCTGGTTCCCTTATTCAAGCGTGGTAACAAACTGTTCATCGGCACTTC
CGATCCTACCCAGACCTCCGCTTTTCATGCCATCACCTTCAAGACGGGACTGACCGTCGAGGTAGTGGTGGTTGAGGATG
ACAAGCTATCCAAGGTGATCGGCAAGTACACCGAAGATGCGTCTGCGGCCATCAAAGCGCTGGAGAATGAAGATTTTGGC
GATCTGGAGTTTGTTGATCCGGATGCACCAACGCCACACAGTGAAACCAATCAACCCGATGTGGATGATGCCCCTGTGGT
CAAATTCATCCACAAGGTCTTGCTGGATGCCATCAATGGCGGTGCCTCCGATGTACATTTCGAGCCTTACGAAAAGTTTT
ACCGCATCCGCTACCGTGTCGATGGCCAGTTGAAGGAAATTGCCCAGCCTCCTTTGCTGCTGAAAGAGAAAATTGCTTCC
CGGATCAAGGTGATTTCACGGCTTGATATTTCTGAAAAGCGCGTACCGCAGGATGGCCGTCTGAAACTGGTATTATCCAA
ATCGCATTCCATTGATTTCCGTGTCAGCACCTTGCCGACCTTGTTCGGTGAAAAAATCGTGATGCGTATTCTGGACTCCT
CGGCAGCATCGTTGGACATTGAGCAGCTGGGGTTTGAGCCGGAACAGAAACAGATGCTGCTGGATGCCATTGGCCGGCCA
TATGGCATGGTGCTGGTAACTGGTCCGACCGGTAGTGGCAAGACGGTATCGCTATATACATGCCTGAATATCCTGAACAA
GCCGGATATCAATATTTCCACGGCGGAAGATCCGGTCGAGATCAACTTGCCTGGTATCAATCAGGTCAACGTCAACGAGA
AGGCTGGGCTGACGTTTTCCTCGGCACTGAAAGCCTTCCTGCGTCAGGATCCGGATGTCATCATGGTGGGTGAAATCCGG
GACTTTGAAACCGCCGATATTTCCATCAAGGCGGCCCAAACAGGTCATATGGTTTTTTCCACCTTGCACACCAATAATGC
GCCGGCTACGCTGACCCGTTTGCTGAACATGGGCGTGGCACCGTTCAATGTGGCCAGCTCGGTATTGCTGATCATGGCGC
AGCGGTTGGCGCGCCGCCTGTGCAATCATTGCAAGGCACCGGTTGATATTCCGGAGCCAGCTCTGCTCAGGGCCGGCTTC
CGTCCTGAAGAGCTGGATGGTAGCTGGAAACCGTACGGGCCTGTCGGTTGTGATGAGTGTCGTGGTTCCGGCTATAAAGG
CCGTACTGGCATTTATGAAGTCATGCCGATCAGTGATGCGATGACACGTTTGATCATGCAAAATGGCACGGCAATCGATA
TCAATGATCTGGCCCTTAAAGAAGGGATGATCGATCTGCGACATGCTGGTCTGCTGAAAGTCAAGAAAGGGCTGACCTCC
TTGGCCGAAATCGAGGCTGTGACCAACGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-147)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

57.319

99.474

0.57
  pilB Acinetobacter baumannii D1279779

54.231

100

0.551
  pilB Acinetobacter baylyi ADP1

54.513

99.123

0.54
  pilB Legionella pneumophila strain ERS1305867

53.263

99.474

0.53
  pilB Vibrio cholerae strain A1552

50.547

96.14

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

47.518

98.947

0.47
  pilB Vibrio campbellii strain DS40M4

46.549

99.123

0.461
  pilF Thermus thermophilus HB27

37.809

99.298

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.098

93.333

0.365

Multiple sequence alignment