Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   M0P28_RS00785 Genome accession   NZ_CP116958
Coordinates   118264..119307 (+) Length   347 a.a.
NCBI ID   WP_003062919.1    Uniprot ID   E0PB48
Organism   Streptococcus pasteurianus strain WUSP074     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 113264..124307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0P28_RS00775 (M0P28_00775) - 116966..117328 (+) 363 WP_058692629.1 DUF1033 family protein -
  M0P28_RS00780 (M0P28_00780) comGA 117399..118340 (+) 942 WP_003062917.1 competence type IV pilus ATPase ComGA Machinery gene
  M0P28_RS00785 (M0P28_00785) comGB 118264..119307 (+) 1044 WP_003062919.1 competence type IV pilus assembly protein ComGB Machinery gene
  M0P28_RS00790 (M0P28_00790) comGC 119307..119600 (+) 294 WP_117479472.1 competence type IV pilus major pilin ComGC Machinery gene
  M0P28_RS00795 (M0P28_00795) comGD 119584..120015 (+) 432 WP_058692630.1 competence type IV pilus minor pilin ComGD Machinery gene
  M0P28_RS00800 (M0P28_00800) comGE 119969..120262 (+) 294 WP_081048608.1 competence type IV pilus minor pilin ComGE Machinery gene
  M0P28_RS00805 (M0P28_00805) comGF 120216..120683 (+) 468 WP_058692632.1 competence type IV pilus minor pilin ComGF Machinery gene
  M0P28_RS00810 (M0P28_00810) comGG 120637..120987 (+) 351 WP_238374707.1 competence type IV pilus minor pilin ComGG Machinery gene
  M0P28_RS00815 (M0P28_00815) comGH 121042..122013 (+) 972 WP_117479470.1 class I SAM-dependent methyltransferase Machinery gene
  M0P28_RS00820 (M0P28_00820) - 122051..123250 (+) 1200 WP_058692635.1 acetate kinase -
  M0P28_RS00825 (M0P28_00825) - 123417..123617 (+) 201 WP_058692636.1 helix-turn-helix transcriptional regulator -
  M0P28_RS00830 (M0P28_00830) - 123627..123833 (+) 207 WP_058692637.1 hypothetical protein -
  M0P28_RS00835 (M0P28_00835) - 123824..124276 (+) 453 WP_117479468.1 ABC transporter permease -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39817.38 Da        Isoelectric Point: 9.8728

>NTDB_id=715221 M0P28_RS00785 WP_003062919.1 118264..119307(+) (comGB) [Streptococcus pasteurianus strain WUSP074]
MQKLKVLLKTDISQLNKQKSKKLPFKKQRKVIQLFNNLFKSGFNLTEIVFFLRRSQLLSEVYVERMQESLLNGASLAAMM
VDLGFSDNIVTQIALADVHGNSQKSLLKIESYLSSMTVVRKKLIEVATYPLILFLFLILIMLGLKNYLLPQLESQNVATQ
IIAHFPTIFLLSIFSIGVLLICTTFYARRLSQIDLYSRISRIPLVGNYVRLYLTAYYAREWGNLIGQGIELMAIVGIMQK
QKSLLFQEIGKDMEEALLSGQAFHQKVLDYPFFLRELSLMIEYGEIKSKLGRELDIYAEETWQSFFGKLTQATQLIQPLV
FVFVALIIVLIYVAMLLPMYQNMGGNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=715221 M0P28_RS00785 WP_003062919.1 118264..119307(+) (comGB) [Streptococcus pasteurianus strain WUSP074]
ATGCAAAAATTGAAAGTCTTGTTAAAGACGGACATATCACAGCTGAACAAGCAAAAATCGAAAAAATTGCCATTTAAAAA
ACAGCGCAAGGTTATCCAACTCTTTAATAATCTTTTTAAAAGTGGGTTTAATTTAACAGAAATTGTGTTTTTTCTCCGAA
GAAGTCAATTGTTGTCAGAGGTTTATGTTGAGAGGATGCAAGAAAGTTTGTTAAATGGTGCTAGTCTAGCAGCAATGATG
GTAGATTTAGGGTTTTCGGACAATATTGTCACACAAATTGCTTTGGCTGATGTTCATGGAAACAGTCAGAAAAGTCTGCT
AAAAATTGAGTCTTACCTTTCTAGCATGACTGTCGTCAGAAAAAAGTTAATTGAAGTTGCAACGTATCCATTGATTTTGT
TCTTGTTTCTTATTTTGATTATGCTGGGGTTGAAGAATTATTTACTGCCGCAGCTGGAAAGCCAAAATGTGGCAACGCAG
ATTATTGCGCATTTTCCAACGATTTTTTTGTTAAGTATTTTCTCGATTGGAGTGTTGCTTATTTGTACGACATTTTATGC
TAGGCGTTTATCGCAGATTGATTTATATAGTCGAATAAGCCGGATTCCACTTGTGGGAAACTATGTTAGGTTATATTTGA
CAGCTTACTATGCGCGTGAATGGGGAAATTTGATTGGGCAAGGTATTGAATTAATGGCAATCGTGGGAATCATGCAAAAG
CAAAAGTCACTCTTATTTCAAGAGATTGGAAAGGATATGGAAGAAGCGCTGCTTTCAGGGCAAGCTTTTCATCAAAAAGT
TCTGGATTATCCATTCTTTTTGCGAGAATTGAGCTTGATGATTGAATATGGTGAGATCAAATCAAAGCTTGGGCGTGAGT
TGGACATTTATGCTGAGGAAACATGGCAGAGCTTTTTTGGCAAATTGACTCAAGCAACACAGCTCATTCAACCACTTGTT
TTTGTCTTTGTAGCTTTGATTATTGTGTTAATTTATGTGGCAATGCTGTTGCCAATGTATCAAAATATGGGAGGAAATTT
TTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0PB48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Streptococcus mutans UA140

66.456

91.066

0.605
  comGB Streptococcus mutans UA159

66.139

91.066

0.602
  comGB/cglB Streptococcus mitis NCTC 12261

59.587

97.695

0.582
  comGB Streptococcus gordonii str. Challis substr. CH1

59.524

96.83

0.576
  comGB/cglB Streptococcus mitis SK321

56.677

97.118

0.55
  comGB/cglB Streptococcus pneumoniae Rx1

55.786

97.118

0.542
  comGB/cglB Streptococcus pneumoniae D39

55.786

97.118

0.542
  comGB/cglB Streptococcus pneumoniae R6

55.786

97.118

0.542
  comGB/cglB Streptococcus pneumoniae TIGR4

55.786

97.118

0.542
  comGB Lactococcus lactis subsp. cremoris KW2

50

98.559

0.493