Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J62_RS03685 Genome accession   NZ_AP018779
Coordinates   726510..727904 (+) Length   464 a.a.
NCBI ID   WP_110432057.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-8 isolate 10H15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721510..732904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J62_RS03660 (CHBNIII8_06950) rsmE 721526..722263 (-) 738 WP_110432052.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J62_RS03665 (CHBNIII8_06960) lnt 722313..723842 (-) 1530 WP_110432053.1 apolipoprotein N-acyltransferase -
  K6J62_RS03670 (CHBNIII8_06970) corC 723865..724764 (-) 900 WP_110432054.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J62_RS03675 (CHBNIII8_06980) ampD 725396..725950 (-) 555 WP_110432055.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J62_RS03680 (CHBNIII8_06990) pilA 726064..726513 (+) 450 WP_110432056.1 prepilin peptidase-dependent pilin Machinery gene
  K6J62_RS03685 (CHBNIII8_07000) pilB 726510..727904 (+) 1395 WP_110432057.1 GspE/PulE family protein Machinery gene
  K6J62_RS03690 (CHBNIII8_07010) pilC 727901..729121 (+) 1221 WP_110432058.1 type II secretion system F family protein Machinery gene
  K6J62_RS03695 (CHBNIII8_07020) pilD 729118..729810 (+) 693 WP_110432059.1 A24 family peptidase Machinery gene
  K6J62_RS03700 (CHBNIII8_07030) rho 729864..731126 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J62_RS03705 (CHBNIII8_07040) metJ 731373..731690 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J62_RS03710 (CHBNIII8_07050) cueR 731704..732090 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  K6J62_RS03715 (CHBNIII8_07060) - 732167..732373 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  K6J62_RS03720 (CHBNIII8_07070) - 732447..732653 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52872.31 Da        Isoelectric Point: 5.4816

>NTDB_id=71252 K6J62_RS03685 WP_110432057.1 726510..727904(+) (pilB) [Haemophilus influenzae strain CHBN-III-8 isolate 10H15]
MTSYALLHTQRVIAQNGEVFTISPNLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLANSCDCHQGYKGRIGVYQFLHWQQNGYQTDFENLRDSGLEKVNQGMTDNKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=71252 K6J62_RS03685 WP_110432057.1 726510..727904(+) (pilB) [Haemophilus influenzae strain CHBN-III-8 isolate 10H15]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGGTATTTACGATCTCGCCAAATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTTTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTTTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAGCAACTTACTCCGTGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCTTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGTGCGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCCACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATCATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGCCAAGATCCCGACATCATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACCGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCTATATCTCGCTTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAGCAAATTCTTGTGATTGCCATCAAGGTTATAAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAAAATGGCTATCAAACGGATTTTGAGAATTTACGAGATAGCGGTCTAGAAAAAGTTAATCAAGGAATGACAGATAATA
AAGAAATTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-95)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.983

100

0.97
  pilB Haemophilus influenzae Rd KW20

96.328

99.784

0.961
  pilB Glaesserella parasuis strain SC1401

57.391

99.138

0.569
  pilB Vibrio cholerae strain A1552

39.279

100

0.446
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Legionella pneumophila strain ERS1305867

38.812

100

0.422
  pilB Acinetobacter baylyi ADP1

39.113

100

0.418
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.416
  pilB Acinetobacter baumannii D1279779

39.588

100

0.414
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.607

100

0.379
  pilF Thermus thermophilus HB27

35.924

100

0.369

Multiple sequence alignment