Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6J69_RS03790 Genome accession   NZ_AP018777
Coordinates   762591..763040 (-) Length   149 a.a.
NCBI ID   WP_012054948.1    Uniprot ID   A0AAX3ITG0
Organism   Haemophilus influenzae strain CHBN-III-6 isolate 13H31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 757591..768040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J69_RS03770 (CHBNIII6_07180) rho 757978..759240 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J69_RS03775 (CHBNIII6_07190) pilD 759294..759986 (-) 693 WP_050848919.1 A24 family peptidase Machinery gene
  K6J69_RS03780 (CHBNIII6_07200) pilC 759983..761203 (-) 1221 WP_110431166.1 type II secretion system F family protein Machinery gene
  K6J69_RS03785 (CHBNIII6_07210) pilB 761200..762594 (-) 1395 WP_110431165.1 GspE/PulE family protein Machinery gene
  K6J69_RS03790 (CHBNIII6_07220) pilA 762591..763040 (-) 450 WP_012054948.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J69_RS03795 (CHBNIII6_07230) ampD 763155..763709 (+) 555 WP_110431164.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J69_RS03800 (CHBNIII6_07240) corC 764342..765241 (+) 900 WP_005662987.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J69_RS03805 (CHBNIII6_07250) lnt 765264..766793 (+) 1530 WP_110431168.1 apolipoprotein N-acyltransferase -
  K6J69_RS03810 (CHBNIII6_07260) rsmE 766843..767580 (+) 738 WP_040034311.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15523.99 Da        Isoelectric Point: 9.6179

>NTDB_id=71183 K6J69_RS03790 WP_012054948.1 762591..763040(-) (pilA) [Haemophilus influenzae strain CHBN-III-6 isolate 13H31]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSTCSGGSNGIA
ADITTAKGYVASVKTQSGGITVKGNGTLANMEYILQAKGNATAGVTWTTTCKGTDASLFPANFCRSVTK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=71183 K6J69_RS03790 WP_012054948.1 762591..763040(-) (pilA) [Haemophilus influenzae strain CHBN-III-6 isolate 13H31]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCTTCTGCGC
CTTATAAGGCTGATGTGGAATTATGCGTTTATAGCACAGGCAAACCTTCTACTTGCTCAGGAGGAAGCAATGGAATTGCA
GCTGATATTACAACAGCAAAAGGCTATGTAGCCTCAGTGAAAACTCAATCAGGTGGTATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTAAAGGTAATGCTACAGCAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCAGAAGTGTCACAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

91.946

100

0.919
  pilA Haemophilus influenzae 86-028NP

87.919

100

0.879
  pilA Glaesserella parasuis strain SC1401

56.757

99.329

0.564
  pilA2 Legionella pneumophila strain ERS1305867

39.735

100

0.403
  pilA2 Legionella pneumophila str. Paris

40.559

95.973

0.389
  comP Acinetobacter baylyi ADP1

38.621

97.315

0.376
  pilA Vibrio campbellii strain DS40M4

39.007

94.631

0.369

Multiple sequence alignment