Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J69_RS03785 Genome accession   NZ_AP018777
Coordinates   761200..762594 (-) Length   464 a.a.
NCBI ID   WP_110431165.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-6 isolate 13H31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 756200..767594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J69_RS03750 (CHBNIII6_07140) - 756447..756653 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  K6J69_RS03755 (CHBNIII6_07150) - 756739..756945 (-) 207 WP_005686717.1 heavy-metal-associated domain-containing protein -
  K6J69_RS03760 (CHBNIII6_07160) - 757022..757399 (+) 378 WP_050848920.1 Cu(I)-responsive transcriptional regulator -
  K6J69_RS03765 (CHBNIII6_07170) metJ 757413..757730 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J69_RS03770 (CHBNIII6_07180) rho 757978..759240 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J69_RS03775 (CHBNIII6_07190) pilD 759294..759986 (-) 693 WP_050848919.1 A24 family peptidase Machinery gene
  K6J69_RS03780 (CHBNIII6_07200) pilC 759983..761203 (-) 1221 WP_110431166.1 type II secretion system F family protein Machinery gene
  K6J69_RS03785 (CHBNIII6_07210) pilB 761200..762594 (-) 1395 WP_110431165.1 GspE/PulE family protein Machinery gene
  K6J69_RS03790 (CHBNIII6_07220) pilA 762591..763040 (-) 450 WP_012054948.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J69_RS03795 (CHBNIII6_07230) ampD 763155..763709 (+) 555 WP_110431164.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J69_RS03800 (CHBNIII6_07240) corC 764342..765241 (+) 900 WP_005662987.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J69_RS03805 (CHBNIII6_07250) lnt 765264..766793 (+) 1530 WP_110431168.1 apolipoprotein N-acyltransferase -
  K6J69_RS03810 (CHBNIII6_07260) rsmE 766843..767580 (+) 738 WP_040034311.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52890.45 Da        Isoelectric Point: 5.8432

>NTDB_id=71182 K6J69_RS03785 WP_110431165.1 761200..762594(-) (pilB) [Haemophilus influenzae strain CHBN-III-6 isolate 13H31]
MTSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRVLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAIIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDEKEIERVLGKTL

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=71182 K6J69_RS03785 WP_110431165.1 761200..762594(-) (pilB) [Haemophilus influenzae strain CHBN-III-6 isolate 13H31]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAATTGTTACAACAACTTACTCCGCGTCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGTCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGTGTGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAATGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGCCAAGATCCCGATATTATTATGTTAGGCGAAATTCGCGATGAAGAAAGTGCAATAATTGCATTACGTGC
CGCACAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGCTAGGTAAAACTTTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

97.414

100

0.974
  pilB Haemophilus influenzae 86-028NP

97.408

99.784

0.972
  pilB Glaesserella parasuis strain SC1401

57.301

97.414

0.558
  pilB Vibrio cholerae strain A1552

39.279

100

0.446
  pilB Vibrio campbellii strain DS40M4

40.286

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.351

100

0.418
  pilB Acinetobacter baylyi ADP1

37.903

100

0.405
  pilB Acinetobacter baumannii D1279779

38.462

100

0.399
  pilF Thermus thermophilus HB27

36.157

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.179

100

0.375
  pilF Neisseria gonorrhoeae MS11

36.345

100

0.373

Multiple sequence alignment