Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J64_RS03585 Genome accession   NZ_AP018775
Coordinates   700709..702103 (+) Length   464 a.a.
NCBI ID   WP_011271972.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-4 isolate 12H35     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 695709..707103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J64_RS03560 (CHBNIII4_06830) rsmE 695727..696464 (-) 738 WP_005656289.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J64_RS03565 (CHBNIII4_06840) lnt 696514..698043 (-) 1530 WP_080348255.1 apolipoprotein N-acyltransferase -
  K6J64_RS03570 (CHBNIII4_06850) corC 698066..698965 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J64_RS03575 (CHBNIII4_06860) ampD 699588..700148 (-) 561 WP_014550480.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J64_RS03580 (CHBNIII4_06870) pilA 700263..700712 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J64_RS03585 (CHBNIII4_06880) pilB 700709..702103 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  K6J64_RS03590 (CHBNIII4_06890) pilC 702100..703317 (+) 1218 WP_014550481.1 type II secretion system F family protein Machinery gene
  K6J64_RS03595 (CHBNIII4_06900) pilD 703314..704006 (+) 693 WP_014550482.1 A24 family peptidase Machinery gene
  K6J64_RS03600 (CHBNIII4_06910) rho 704060..705322 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J64_RS03605 (CHBNIII4_06920) metJ 705570..705887 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J64_RS03610 (CHBNIII4_06930) cueR 705901..706287 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  K6J64_RS03615 (CHBNIII4_06940) - 706364..706570 (+) 207 WP_005666692.1 heavy-metal-associated domain-containing protein -
  K6J64_RS03620 (CHBNIII4_06950) - 706656..706862 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52962.30 Da        Isoelectric Point: 5.6248

>NTDB_id=71113 K6J64_RS03585 WP_011271972.1 700709..702103(+) (pilB) [Haemophilus influenzae strain CHBN-III-4 isolate 12H35]
MTSYALLHTQRVTAQNGEIFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPHQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRSLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=71113 K6J64_RS03585 WP_011271972.1 700709..702103(+) (pilB) [Haemophilus influenzae strain CHBN-III-4 isolate 12H35]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCTCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCACAGG
AAGTGGGAAAAGTATCTCGCTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAAGATC
CCATTGAAATTGAACTTGATGGTATTATTCAAAGCCAAATTAATCCGCAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTGGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-98)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

100

100

1
  pilB Haemophilus influenzae Rd KW20

96.544

99.784

0.963
  pilB Glaesserella parasuis strain SC1401

57.456

98.276

0.565
  pilB Vibrio cholerae strain A1552

39.658

100

0.45
  pilB Vibrio campbellii strain DS40M4

40.082

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.416
  pilB Acinetobacter baylyi ADP1

38.105

100

0.407
  pilB Acinetobacter baumannii D1279779

38.669

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Neisseria gonorrhoeae MS11

36.555

100

0.375
  pilF Thermus thermophilus HB27

36.134

100

0.371

Multiple sequence alignment