Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J70_RS03615 Genome accession   NZ_AP018774
Coordinates   712359..713756 (+) Length   465 a.a.
NCBI ID   WP_011961745.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-3 isolate 13H42     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 707359..718756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J70_RS03590 (CHBNIII3_06900) rsmE 707377..708114 (-) 738 WP_005686736.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J70_RS03595 (CHBNIII3_06910) lnt 708164..709693 (-) 1530 WP_074032781.1 apolipoprotein N-acyltransferase -
  K6J70_RS03600 (CHBNIII3_06920) corC 709716..710615 (-) 900 WP_011961742.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J70_RS03605 (CHBNIII3_06930) ampD 711238..711798 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J70_RS03610 (CHBNIII3_06940) pilA 711913..712362 (+) 450 WP_005686726.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J70_RS03615 (CHBNIII3_06950) pilB 712359..713756 (+) 1398 WP_011961745.1 GspE/PulE family protein Machinery gene
  K6J70_RS03620 (CHBNIII3_06960) pilC 713753..714970 (+) 1218 WP_005686722.1 type II secretion system F family protein Machinery gene
  K6J70_RS03625 (CHBNIII3_06970) pilD 714967..715659 (+) 693 WP_005686720.1 A24 family peptidase Machinery gene
  K6J70_RS03630 (CHBNIII3_06980) rho 715713..716975 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J70_RS03635 (CHBNIII3_06990) metJ 717223..717540 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J70_RS03640 (CHBNIII3_07000) cueR 717554..717940 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  K6J70_RS03645 (CHBNIII3_07010) - 718017..718223 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  K6J70_RS03650 (CHBNIII3_07020) - 718309..718515 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53091.42 Da        Isoelectric Point: 5.6399

>NTDB_id=71078 K6J70_RS03615 WP_011961745.1 712359..713756(+) (pilB) [Haemophilus influenzae strain CHBN-III-3 isolate 13H42]
MTSYALLHTQRVTAQNGEIFTISSDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETISFITGKPVEPIL
LESSQLKELLQQLTPHQMQEEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGV
LQPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAEL
GMTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSK
CGGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNL

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=71078 K6J70_RS03615 WP_011961745.1 712359..713756(+) (pilB) [Haemophilus influenzae strain CHBN-III-3 isolate 13H42]
ATGACGAGTTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGTCTGATTTATG
GGCGCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTTCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGAGGAAGAGCAAGTTAAATATTA
CCAACATCAAGAACAGTCTCATCTTGAACAACAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTG
CCTTACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTT
TTACAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATAT
TAGTGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAA
CCTTACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTG
GGTATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGGCCCCAC
AGGAAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACACCTGATAAACATATTATGACTGCTGAGG
ATCCCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTG
CGCACTTTTTTACGTCAAGATCCCGATATCATTATGCTAGGTGAAATTCGCGATGAAGAAAGTGCAAGGATTGCATTACG
TGCCGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGTTTACAACAAC
TCGGTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAACGTCTTGTACGAAAGCTTTGTTCAAAG
TGCGGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTG
GCAACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATG
AGAAAGAAATTGAACGTGTGTTAGGTAAAAACTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-98)

Type II/IV secretion system protein (T2SSE)

(129-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.983

99.785

0.968
  pilB Haemophilus influenzae Rd KW20

95.484

100

0.955
  pilB Glaesserella parasuis strain SC1401

58.515

98.495

0.576
  pilB Vibrio cholerae strain A1552

40.607

100

0.46
  pilB Vibrio campbellii strain DS40M4

40.9

100

0.43
  pilB Vibrio parahaemolyticus RIMD 2210633

40.58

100

0.422
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.415
  pilB Acinetobacter baylyi ADP1

38.384

100

0.409
  pilB Acinetobacter baumannii D1279779

38.763

100

0.404
  pilF Neisseria gonorrhoeae MS11

37.605

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.74

100

0.381
  pilF Thermus thermophilus HB27

35.789

100

0.366

Multiple sequence alignment