Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J68_RS03600 Genome accession   NZ_AP018773
Coordinates   708368..709771 (+) Length   467 a.a.
NCBI ID   WP_105197617.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-2 isolate 13H39     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 703368..714771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J68_RS03575 (CHBNIII2_06890) rsmE 703386..704123 (-) 738 WP_005686736.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J68_RS03580 (CHBNIII2_06900) lnt 704173..705702 (-) 1530 WP_074032781.1 apolipoprotein N-acyltransferase -
  K6J68_RS03585 (CHBNIII2_06910) corC 705725..706624 (-) 900 WP_011961742.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J68_RS03590 (CHBNIII2_06920) ampD 707247..707807 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J68_RS03595 (CHBNIII2_06930) pilA 707922..708371 (+) 450 WP_005686726.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J68_RS03600 (CHBNIII2_06940) pilB 708368..709771 (+) 1404 WP_105197617.1 GspE/PulE family protein Machinery gene
  K6J68_RS03605 (CHBNIII2_06950) pilC 709768..710985 (+) 1218 WP_005686722.1 type II secretion system F family protein Machinery gene
  K6J68_RS03610 (CHBNIII2_06960) pilD 710982..711674 (+) 693 WP_005686720.1 A24 family peptidase Machinery gene
  K6J68_RS03615 (CHBNIII2_06970) rho 711728..712990 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J68_RS03620 (CHBNIII2_06980) metJ 713238..713555 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J68_RS03625 (CHBNIII2_06990) cueR 713569..713955 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  K6J68_RS03630 (CHBNIII2_07000) - 714032..714238 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  K6J68_RS03635 (CHBNIII2_07010) - 714324..714530 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 53319.62 Da        Isoelectric Point: 5.3433

>NTDB_id=71042 K6J68_RS03600 WP_105197617.1 708368..709771(+) (pilB) [Haemophilus influenzae strain CHBN-III-2 isolate 13H39]
MTSYALLHTQRVTAQNGEIFTISSDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETISFITGKPVEPIL
LESSQLKELLQQLTPHQMQEEEEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRID
GVLQPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFA
ELGMTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSR
LLRAFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLC
SKCGGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNL

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=71042 K6J68_RS03600 WP_105197617.1 708368..709771(+) (pilB) [Haemophilus influenzae strain CHBN-III-2 isolate 13H39]
ATGACGAGTTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGTCTGATTTATG
GGCGCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTTCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGAGGAAGAGGAAGAGCAAGTTAA
ATATTACCAACATCAAGAACAGTCTCATCTTGAACAACAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTG
AATCTGCCTTACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGAT
GGTGTTTTACAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATT
AGATATTAGTGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCC
TTTCAACCTTACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCT
GAACTGGGTATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGG
CCCCACAGGAAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACACCTGATAAACATATTATGACTG
CTGAGGATCCCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGT
CTATTGCGCGCTTTTTTACGTCAAGATCCCGATATCATTATGCTAGGTGAAATTCGCGATGAAGAAAGTGCAAGGATTGC
ATTACGTGCCGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGTTTAC
AACAACTCGGTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAACGTCTTGTACGAAAGCTTTGT
TCAAAGTGCGGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCT
ACATTGGCAACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAA
CAGATGAGAAAGAAATTGAACGTGTGTTAGGTAAAAACTTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(131-398)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.781

99.786

0.966
  pilB Haemophilus influenzae Rd KW20

94.861

100

0.949
  pilB Glaesserella parasuis strain SC1401

58.297

98.073

0.572
  pilB Vibrio cholerae strain A1552

40.797

100

0.46
  pilB Vibrio campbellii strain DS40M4

40.695

100

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.415
  pilB Acinetobacter baylyi ADP1

38.855

100

0.407
  pilB Acinetobacter baumannii D1279779

39.256

100

0.407
  pilF Neisseria gonorrhoeae MS11

37.657

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.953

100

0.381
  pilF Thermus thermophilus HB27

36.421

100

0.37

Multiple sequence alignment