Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6J68_RS03595 Genome accession   NZ_AP018773
Coordinates   707922..708371 (+) Length   149 a.a.
NCBI ID   WP_005686726.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-2 isolate 13H39     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 702922..713371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J68_RS03575 (CHBNIII2_06890) rsmE 703386..704123 (-) 738 WP_005686736.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J68_RS03580 (CHBNIII2_06900) lnt 704173..705702 (-) 1530 WP_074032781.1 apolipoprotein N-acyltransferase -
  K6J68_RS03585 (CHBNIII2_06910) corC 705725..706624 (-) 900 WP_011961742.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J68_RS03590 (CHBNIII2_06920) ampD 707247..707807 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J68_RS03595 (CHBNIII2_06930) pilA 707922..708371 (+) 450 WP_005686726.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J68_RS03600 (CHBNIII2_06940) pilB 708368..709771 (+) 1404 WP_105197617.1 GspE/PulE family protein Machinery gene
  K6J68_RS03605 (CHBNIII2_06950) pilC 709768..710985 (+) 1218 WP_005686722.1 type II secretion system F family protein Machinery gene
  K6J68_RS03610 (CHBNIII2_06960) pilD 710982..711674 (+) 693 WP_005686720.1 A24 family peptidase Machinery gene
  K6J68_RS03615 (CHBNIII2_06970) rho 711728..712990 (-) 1263 WP_005648966.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15542.90 Da        Isoelectric Point: 8.4607

>NTDB_id=71041 K6J68_RS03595 WP_005686726.1 707922..708371(+) (pilA) [Haemophilus influenzae strain CHBN-III-2 isolate 13H39]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNEATKCTGGKNGIA
ADITTAKGYVKSVTTSNGAITVKGDGTLANMEYILQASGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=71041 K6J68_RS03595 WP_005686726.1 707922..708371(+) (pilA) [Haemophilus influenzae strain CHBN-III-2 isolate 13H39]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATCCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAGCAACAAAGTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCCAGTGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

97.987

100

0.98
  pilA Haemophilus influenzae Rd KW20

91.275

100

0.913
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila strain ERS1305867

42.446

93.289

0.396
  pilA2 Legionella pneumophila str. Paris

42.446

93.289

0.396
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376

Multiple sequence alignment