Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NGC85_RS01660 Genome accession   NZ_CP101115
Coordinates   360316..360807 (+) Length   163 a.a.
NCBI ID   WP_254804642.1    Uniprot ID   -
Organism   Acinetobacter sp. Z1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 355316..365807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGC85_RS01635 (NGC85_01635) - 355855..356745 (+) 891 WP_005059930.1 metal-dependent hydrolase -
  NGC85_RS01640 (NGC85_01640) - 356924..357514 (+) 591 WP_254804638.1 LemA family protein -
  NGC85_RS01645 (NGC85_01645) - 357551..358600 (+) 1050 WP_254804639.1 YgcG family protein -
  NGC85_RS01650 (NGC85_01650) - 358594..359154 (+) 561 WP_254804640.1 TPM domain-containing protein -
  NGC85_RS01655 (NGC85_01655) pilA 359551..360042 (+) 492 WP_254804641.1 pilin Machinery gene
  NGC85_RS01660 (NGC85_01660) pilA 360316..360807 (+) 492 WP_254804642.1 pilin Machinery gene
  NGC85_RS01665 (NGC85_01665) - 360830..362518 (+) 1689 WP_254804643.1 PglL family O-oligosaccharyltransferase -
  NGC85_RS01670 (NGC85_01670) tfpZ 362582..363316 (+) 735 WP_254804644.1 TfpX/TfpZ family type IV pilin accessory protein -
  NGC85_RS01675 (NGC85_01675) - 363398..365038 (+) 1641 WP_254804645.1 PglL family O-oligosaccharyltransferase -
  NGC85_RS01680 (NGC85_01680) bfr 365100..365564 (-) 465 WP_005052191.1 bacterioferritin -

Sequence

Protein


Download         Length: 163 a.a.        Molecular weight: 16767.33 Da        Isoelectric Point: 8.4892

>NTDB_id=708720 NGC85_RS01660 WP_254804642.1 360316..360807(+) (pilA) [Acinetobacter sp. Z1]
MHTMQKGFTLIELMIVVAIIGTLAAIAIPAYQDYTVRSKVTEGLSLASSAKVVVSENAVNSVAFGNSWVAPTATLNVSNI
EINSTNGRITISYTTAVAPVGANTLVISPLNGDRMTGTALVLNIPPSLGSITWVCRSANATTTVAAAGVGTLAAKYAPAE
CRN

Nucleotide


Download         Length: 492 bp        

>NTDB_id=708720 NGC85_RS01660 WP_254804642.1 360316..360807(+) (pilA) [Acinetobacter sp. Z1]
ATGCACACCATGCAAAAAGGCTTTACACTTATTGAATTAATGATTGTTGTCGCAATTATTGGTACTCTTGCAGCAATTGC
AATTCCTGCTTATCAAGATTATACAGTGAGATCAAAGGTAACAGAAGGATTGTCTTTAGCTTCAAGCGCAAAAGTTGTGG
TGTCAGAAAATGCTGTGAATAGCGTTGCATTTGGAAATAGTTGGGTCGCACCAACAGCTACTTTAAATGTATCTAATATT
GAAATTAACTCTACTAATGGCCGTATTACAATATCATATACAACTGCAGTTGCACCAGTTGGTGCAAATACATTAGTTAT
AAGTCCATTAAATGGAGATAGGATGACTGGTACAGCATTAGTATTGAACATTCCTCCGTCATTAGGATCTATCACATGGG
TATGCCGTTCAGCGAATGCAACTACAACAGTGGCAGCAGCCGGTGTTGGGACGCTTGCAGCTAAATATGCACCTGCGGAA
TGTCGTAATTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-162)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

55.357

100

0.571
  pilA2 Legionella pneumophila str. Paris

49.398

100

0.503
  pilA2 Legionella pneumophila strain ERS1305867

49.693

100

0.497
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.741

100

0.472
  comP Acinetobacter baylyi ADP1

41.975

99.387

0.417
  pilE Neisseria gonorrhoeae strain FA1090

35.429

100

0.38
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.364

100

0.368
  pilE Neisseria gonorrhoeae MS11

34.682

100

0.368