Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NL778_RS01770 Genome accession   NZ_CP100652
Coordinates   376048..377316 (+) Length   422 a.a.
NCBI ID   WP_254467025.1    Uniprot ID   -
Organism   Pseudomonas putida strain HSM-C2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 371048..382316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL778_RS01750 (NL778_01750) - 371094..373547 (-) 2454 WP_012312324.1 penicillin-binding protein 1A -
  NL778_RS01755 (NL778_01755) pilM 373727..374575 (+) 849 WP_254467023.1 type IV pilus biogenesis protein PilM -
  NL778_RS01760 (NL778_01760) - 374575..375111 (+) 537 WP_012312327.1 PilN domain-containing protein -
  NL778_RS01765 (NL778_01765) - 375285..376124 (+) 840 WP_254467024.1 pilus assembly protein PilP -
  NL778_RS01770 (NL778_01770) pilQ 376048..377316 (+) 1269 WP_254467025.1 type IV pilus secretin PilQ Machinery gene
  NL778_RS01775 (NL778_01775) aroK 377321..377839 (+) 519 WP_039602073.1 shikimate kinase AroK -
  NL778_RS01780 (NL778_01780) aroB 377934..379031 (+) 1098 WP_254467026.1 3-dehydroquinate synthase -
  NL778_RS01785 (NL778_01785) - 379047..380627 (+) 1581 WP_012312332.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45975.82 Da        Isoelectric Point: 4.6998

>NTDB_id=706051 NL778_RS01770 WP_254467025.1 376048..377316(+) (pilQ) [Pseudomonas putida strain HSM-C2]
MNGLMKWLVAALLPMPLLAATPYRGEPISLNFQDVEVRLVLQVLADYAGINLVASDTVQGSITLRLQDVPWDQALELVLR
SKGLGRREEGKVLLVAPAAELAEQALGARMGLELEAQSQPLRRELLPIHHAEASELAELLLATLADDGILTGRGSLSVDV
RTNTLVAHQPEDRLAELRQLVAQLDVPVRQVEIEARIVEANVDYENSLGVRWGPRLYSEAPALGKDVFVDLGMERAGSSI
GFGVLRGGVLLDLELSAMEKTGNGEIISQPKVVTANNETARILKGTEVPYQETTKSGATSVTFREASLSLEVTPQITPDG
KVIMTVKVTKDEPDFVNALNDVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMNDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=706051 NL778_RS01770 WP_254467025.1 376048..377316(+) (pilQ) [Pseudomonas putida strain HSM-C2]
ATGAACGGGTTGATGAAGTGGCTGGTGGCGGCATTGCTGCCGATGCCGTTGCTGGCGGCAACGCCTTATCGGGGTGAACC
GATTTCGCTGAACTTCCAGGACGTAGAAGTGCGCCTTGTGTTGCAGGTGCTTGCCGATTATGCCGGGATCAATCTGGTCG
CAAGCGACACGGTGCAGGGCAGTATTACCTTGCGTCTGCAGGATGTTCCTTGGGATCAGGCTCTGGAACTGGTGTTGCGC
AGCAAAGGGCTGGGCCGGCGGGAGGAGGGCAAGGTGCTGTTGGTGGCGCCAGCGGCTGAGCTGGCCGAACAGGCTCTGGG
AGCGCGCATGGGGTTGGAGCTGGAGGCCCAATCGCAGCCGTTGCGCCGCGAGTTGCTGCCGATACACCACGCCGAGGCCT
CGGAACTTGCCGAGTTGCTGCTGGCTACGTTGGCCGATGACGGCATTCTGACTGGCCGCGGTAGTTTGAGTGTGGATGTG
CGCACCAATACGCTGGTTGCGCACCAGCCTGAGGACCGGCTTGCCGAGTTGCGCCAGTTGGTTGCGCAACTGGATGTGCC
GGTACGACAGGTAGAGATCGAGGCGCGCATCGTGGAGGCCAATGTCGATTACGAGAACAGCCTTGGGGTGCGCTGGGGCC
CTCGCCTCTACAGCGAAGCCCCTGCTTTGGGCAAGGACGTGTTTGTTGATCTTGGCATGGAGCGGGCCGGCTCCAGCATC
GGCTTTGGAGTGTTGCGTGGGGGCGTGCTGTTGGACCTTGAACTCAGCGCCATGGAGAAGACCGGCAATGGTGAAATCAT
TTCCCAGCCCAAGGTGGTCACCGCTAACAACGAAACTGCCCGCATCCTCAAAGGTACCGAAGTCCCTTACCAGGAGACCA
CGAAGAGTGGCGCTACGTCGGTTACATTCCGCGAGGCGTCGCTGTCGTTGGAGGTCACTCCGCAGATCACGCCGGACGGC
AAGGTGATCATGACGGTCAAGGTGACCAAGGACGAGCCTGATTTCGTCAATGCCTTGAACGATGTGCCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTACGGGTAGCTGACGGGGAAACCATCGTCATTGGCGGTGTGTATTCGACTACGCAAAACA
ATGTGGTGGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAATGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.503

100

0.611
  comQ Acinetobacter baylyi ADP1

41.372

100

0.443
  pilQ Acinetobacter baumannii D1279779

39.871

100

0.438
  pilQ Acinetobacter baumannii strain A118

39.871

100

0.438
  pilQ Legionella pneumophila strain Lp02

42.993

99.763

0.429
  pilQ Legionella pneumophila strain ERS1305867

42.993

99.763

0.429
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.005

99.289

0.417
  pilQ Vibrio cholerae strain A1552

42.005

99.289

0.417
  pilQ Vibrio campbellii strain DS40M4

40.662

100

0.408
  pilQ Neisseria gonorrhoeae strain FA1090

36.407

100

0.365