Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NKI27_RS14015 Genome accession   NZ_CP100390
Coordinates   3092024..3093250 (+) Length   408 a.a.
NCBI ID   WP_265046655.1    Uniprot ID   -
Organism   Alkalimarinus alittae strain A2M4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3087024..3098250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKI27_RS13985 (NKI27_13955) - 3087039..3089024 (-) 1986 WP_265046653.1 hypothetical protein -
  NKI27_RS13990 (NKI27_13960) - 3088987..3089433 (-) 447 WP_320109427.1 pilin -
  NKI27_RS14000 (NKI27_13970) - 3089505..3089975 (-) 471 WP_320109428.1 pilin -
  NKI27_RS14010 (NKI27_13980) pilB 3090312..3092021 (+) 1710 WP_265046654.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NKI27_RS14015 (NKI27_13985) pilC 3092024..3093250 (+) 1227 WP_265046655.1 type II secretion system F family protein Machinery gene
  NKI27_RS14020 (NKI27_13990) pilD 3093317..3094186 (+) 870 WP_265046656.1 A24 family peptidase Machinery gene
  NKI27_RS14025 (NKI27_13995) - 3094234..3094809 (+) 576 WP_265046657.1 AAA family ATPase -
  NKI27_RS14030 (NKI27_14000) - 3094809..3094982 (+) 174 WP_265046658.1 hypothetical protein -
  NKI27_RS14035 (NKI27_14005) coaE 3095019..3095606 (+) 588 WP_265046659.1 dephospho-CoA kinase -
  NKI27_RS14040 (NKI27_14010) yacG 3095637..3095864 (+) 228 WP_265046660.1 DNA gyrase inhibitor YacG -
  NKI27_RS14045 (NKI27_14015) - 3095884..3096330 (+) 447 WP_265046661.1 HIT family protein -
  NKI27_RS14050 (NKI27_14020) - 3096345..3096704 (+) 360 WP_265046662.1 CidA/LrgA family protein -
  NKI27_RS14055 (NKI27_14025) - 3096701..3097420 (+) 720 WP_265046663.1 LrgB family protein -
  NKI27_RS14060 (NKI27_14030) tsaA 3097433..3098146 (+) 714 WP_265046664.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44230.50 Da        Isoelectric Point: 7.3563

>NTDB_id=704817 NKI27_RS14015 WP_265046655.1 3092024..3093250(+) (pilC) [Alkalimarinus alittae strain A2M4]
MAEKSEKLGSFTWEGTDRKGNKTKGKTSGKNAALIKAQLRQQGITPTKVKKEAVSLSFGGKGKAITPFDIAIFTRQLATM
MKAGVPLIQSFDIVADGLENPNLRDIVLDIKTEVAAGNNFAGALRRHPKHFDDLFCNLVNSGEQSGALETMLDRVATYME
KTETLKRKVKKAMTYPIAVVIVALVVTTILLVKVVPQFEDLFSGFGADLPAFTQFVIGISEWMQEWWFVALGIIIATVYT
FKESKRRSPAFADKVDHFMLKLPVVGVIIDKSAVARFGRVLSTTFAAGVPLVDALDSVAGAAGNAKYRDAIHTIRDDVSS
GTQLQFSMKSTAVFPSMAVQLVAIGEESGALDSMLEKVADYYEEEVDTLVDSLTSLMEPVIMSVLGVLVGGLIVAMYLPI
FQMGQAVG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=704817 NKI27_RS14015 WP_265046655.1 3092024..3093250(+) (pilC) [Alkalimarinus alittae strain A2M4]
ATGGCAGAAAAATCAGAAAAGTTAGGCTCCTTCACTTGGGAAGGTACAGACCGCAAAGGTAATAAAACCAAAGGCAAAAC
GAGCGGGAAAAATGCGGCATTGATTAAAGCACAACTACGACAACAAGGAATAACGCCCACTAAAGTAAAAAAAGAAGCGG
TTTCCTTATCCTTTGGTGGGAAAGGGAAGGCAATAACACCCTTTGATATCGCTATTTTTACTCGCCAATTGGCGACCATG
ATGAAAGCGGGCGTTCCGCTAATTCAGTCCTTCGATATAGTTGCAGACGGGCTCGAAAACCCAAACCTTCGTGATATAGT
ACTGGATATAAAAACTGAGGTTGCAGCAGGTAATAATTTTGCAGGAGCCCTTCGACGGCACCCAAAACACTTCGACGATC
TATTCTGCAATCTAGTAAACTCTGGCGAACAGTCTGGCGCTCTAGAAACCATGTTAGATCGAGTAGCGACCTATATGGAA
AAAACAGAAACACTAAAGAGAAAAGTCAAAAAGGCCATGACTTACCCAATCGCAGTCGTTATTGTTGCATTGGTAGTGAC
TACAATACTCCTTGTCAAAGTTGTACCACAATTCGAGGATCTTTTTTCCGGATTCGGAGCAGACCTGCCTGCATTCACTC
AATTTGTCATAGGCATATCCGAATGGATGCAAGAGTGGTGGTTTGTTGCTTTAGGAATTATTATAGCCACAGTCTACACA
TTTAAAGAAAGCAAAAGAAGGTCTCCTGCTTTCGCGGATAAAGTAGATCACTTTATGCTTAAGTTACCCGTTGTAGGGGT
CATTATAGACAAATCTGCGGTAGCTCGATTTGGCCGAGTTTTATCAACAACATTTGCTGCTGGCGTCCCATTAGTGGATG
CATTAGATTCTGTCGCAGGCGCCGCAGGAAACGCAAAATACCGTGACGCAATACATACAATAAGGGATGATGTTTCATCT
GGGACGCAGCTTCAGTTTTCCATGAAGAGCACAGCTGTGTTTCCTTCTATGGCCGTTCAACTAGTAGCAATCGGCGAGGA
ATCTGGCGCCCTTGACTCTATGCTAGAAAAAGTTGCTGATTACTATGAAGAAGAAGTTGACACTTTGGTTGATAGTTTAA
CAAGCCTTATGGAGCCCGTAATAATGTCTGTATTAGGTGTTCTCGTAGGTGGCTTAATTGTTGCCATGTACCTCCCAATA
TTCCAGATGGGACAAGCCGTAGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(73-196)

Type II secretion system (T2SS), protein F (T2SSF)

(279-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

66.093

99.755

0.659
  pilC Acinetobacter baumannii D1279779

60.494

99.265

0.6
  pilC Acinetobacter baylyi ADP1

59.113

99.51

0.588
  pilC Legionella pneumophila strain ERS1305867

54.545

99.755

0.544
  pilG Neisseria meningitidis 44/76-A

41.626

99.51

0.414
  pilG Neisseria gonorrhoeae MS11

41.626

99.51

0.414
  pilC Vibrio campbellii strain DS40M4

40.194

100

0.407
  pilC Vibrio cholerae strain A1552

40.295

99.755

0.402