Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NKI27_RS14010 Genome accession   NZ_CP100390
Coordinates   3090312..3092021 (+) Length   569 a.a.
NCBI ID   WP_265046654.1    Uniprot ID   -
Organism   Alkalimarinus alittae strain A2M4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3085312..3097021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKI27_RS13975 (NKI27_13945) - 3085561..3086169 (-) 609 WP_265046651.1 class I SAM-dependent methyltransferase -
  NKI27_RS13980 (NKI27_13950) - 3086180..3087046 (-) 867 WP_265046652.1 glycosyltransferase family 2 protein -
  NKI27_RS13985 (NKI27_13955) - 3087039..3089024 (-) 1986 WP_265046653.1 hypothetical protein -
  NKI27_RS13990 (NKI27_13960) - 3088987..3089433 (-) 447 WP_320109427.1 pilin -
  NKI27_RS14000 (NKI27_13970) - 3089505..3089975 (-) 471 WP_320109428.1 pilin -
  NKI27_RS14010 (NKI27_13980) pilB 3090312..3092021 (+) 1710 WP_265046654.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NKI27_RS14015 (NKI27_13985) pilC 3092024..3093250 (+) 1227 WP_265046655.1 type II secretion system F family protein Machinery gene
  NKI27_RS14020 (NKI27_13990) pilD 3093317..3094186 (+) 870 WP_265046656.1 A24 family peptidase Machinery gene
  NKI27_RS14025 (NKI27_13995) - 3094234..3094809 (+) 576 WP_265046657.1 AAA family ATPase -
  NKI27_RS14030 (NKI27_14000) - 3094809..3094982 (+) 174 WP_265046658.1 hypothetical protein -
  NKI27_RS14035 (NKI27_14005) coaE 3095019..3095606 (+) 588 WP_265046659.1 dephospho-CoA kinase -
  NKI27_RS14040 (NKI27_14010) yacG 3095637..3095864 (+) 228 WP_265046660.1 DNA gyrase inhibitor YacG -
  NKI27_RS14045 (NKI27_14015) - 3095884..3096330 (+) 447 WP_265046661.1 HIT family protein -
  NKI27_RS14050 (NKI27_14020) - 3096345..3096704 (+) 360 WP_265046662.1 CidA/LrgA family protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62422.47 Da        Isoelectric Point: 4.9086

>NTDB_id=704816 NKI27_RS14010 WP_265046654.1 3090312..3092021(+) (pilB) [Alkalimarinus alittae strain A2M4]
MASNTSTSLTGLARKFVLDNLLEEQIAKDAFAQANNNNIPFITYLVQNKLAGAKPLAYSAAQEFGMPVMDLSTFMEELIP
EKIVEEKLIRKHHALPLFKRGNRLFVATSDPTNIQALDELKFHTGLSTDAVLVEDDKLGTAIEKYLESQDTSMGDLEDAD
LDDLDVESGESEKDESQGNESDDAPIVKYVNKMLLDAIKGGASDLHFEPYEKTYRVRYRTDGILHEVSKPSIKLAPKIAA
RLKVMCQMDISERRIPQDGRIKLKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKMGIDALGYEEEQKQLYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPETNISTAEDPVEINLEGINQVNVNAKVGLGFPEALRSFLRQDPDIVMVGEIR
DLDTASIAIKAAQTGHLVLSTLHTNSAPETLTRMMNMGVPAFNIATSVSLIIAQRLARRLCSNCKEKADIPKEALLKQGF
TEAQIETGLTLYKPKGCDNCSGGYKGRVGIYEVVKVTPAISQLIMEEATSLDIAAAAQKEGFNNLRQSAMLKAIEGVTSL
EEVNRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=704816 NKI27_RS14010 WP_265046654.1 3090312..3092021(+) (pilB) [Alkalimarinus alittae strain A2M4]
ATGGCATCCAATACATCAACCTCACTTACTGGCTTGGCTCGCAAATTTGTTTTAGACAATCTTCTTGAAGAGCAGATTGC
CAAGGACGCCTTTGCCCAAGCGAACAATAATAACATTCCTTTTATTACCTATTTGGTTCAAAACAAACTAGCAGGTGCAA
AGCCACTGGCATATTCCGCTGCTCAAGAGTTTGGAATGCCGGTGATGGATCTTTCTACTTTTATGGAAGAGTTAATCCCC
GAAAAAATTGTAGAAGAAAAGTTAATAAGAAAGCACCATGCACTCCCACTATTTAAGCGTGGTAACAGACTCTTTGTAGC
AACGTCAGACCCTACAAACATACAGGCGCTGGACGAGCTTAAATTTCATACAGGGTTAAGCACCGATGCAGTACTGGTAG
AAGATGACAAACTTGGAACCGCAATCGAGAAATATCTCGAGTCGCAAGATACTTCTATGGGTGACCTCGAAGATGCTGAC
CTTGATGATCTTGATGTAGAGTCTGGAGAGTCAGAAAAAGACGAGTCACAGGGTAATGAATCAGATGACGCACCTATTGT
TAAATATGTTAACAAGATGCTTTTAGATGCAATAAAAGGTGGCGCTTCTGATCTTCACTTTGAACCCTATGAAAAAACCT
ATAGGGTACGATATAGGACAGATGGAATCTTGCACGAGGTATCAAAACCATCTATTAAATTAGCGCCTAAGATAGCCGCT
CGTTTAAAAGTAATGTGCCAGATGGATATTTCTGAAAGAAGAATCCCGCAAGATGGACGTATAAAGTTAAAACTGTCAAA
AACTAAGGCTATAGATTTTCGTGTTAACACACTACCCACGCTTTGGGGTGAGAAGATAGTACTACGTATTCTTGACCCCA
GTAGTGCAAAAATGGGTATTGATGCGCTTGGGTATGAGGAAGAGCAAAAACAACTGTATATGGACGCTCTTGCCCAACCA
CAAGGGATGATTCTGGTGACAGGCCCAACGGGTAGTGGTAAAACAGTTTCCCTCTATACGGGACTCAACATTCTCAACAC
ACCAGAGACGAATATTTCTACAGCCGAAGACCCTGTTGAAATTAACTTAGAGGGCATTAATCAGGTTAACGTTAATGCCA
AAGTTGGGCTTGGTTTCCCTGAAGCTCTAAGATCTTTCTTACGTCAAGATCCTGACATTGTAATGGTTGGTGAGATTCGA
GATTTAGACACTGCGTCAATTGCTATAAAAGCAGCACAAACAGGTCACCTAGTACTATCCACGCTTCACACAAATAGTGC
ACCCGAAACATTGACCCGTATGATGAATATGGGCGTACCAGCATTTAATATTGCCACCTCCGTCAGTTTGATTATTGCCC
AACGACTCGCTAGGCGCTTATGTAGTAACTGCAAAGAAAAAGCAGACATCCCTAAAGAGGCACTTTTAAAGCAAGGGTTC
ACAGAAGCACAAATTGAAACAGGACTTACGTTGTACAAGCCTAAAGGCTGTGATAATTGTTCAGGAGGCTACAAAGGTCG
AGTGGGTATTTATGAGGTAGTCAAAGTCACACCCGCAATTTCGCAGCTTATTATGGAGGAGGCGACCTCTCTGGATATAG
CAGCTGCAGCCCAAAAAGAAGGGTTTAATAACTTAAGACAGTCTGCAATGCTTAAAGCAATTGAAGGCGTAACAAGCTTG
GAAGAAGTTAACCGAGTAACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60

100

0.601
  pilB Acinetobacter baylyi ADP1

57.821

100

0.578
  pilB Legionella pneumophila strain ERS1305867

55.83

99.473

0.555
  pilB Vibrio cholerae strain A1552

50.264

100

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

50

100

0.501
  pilF Neisseria gonorrhoeae MS11

47.965

99.297

0.476
  pilB Vibrio campbellii strain DS40M4

52.569

88.928

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

40.848

91.213

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.811

93.146

0.371