Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NHU86_RS02225 Genome accession   NZ_CP099742
Coordinates   456654..457895 (-) Length   413 a.a.
NCBI ID   WP_010675822.1    Uniprot ID   A0A2L0TT63
Organism   Aeromonas caviae strain OT55     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 451654..462895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHU86_RS02195 (NHU86_02190) - 452448..453632 (+) 1185 WP_113069549.1 tetratricopeptide repeat protein -
  NHU86_RS02200 (NHU86_02195) mutT 453641..454045 (+) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -
  NHU86_RS02205 (NHU86_02200) yacG 454103..454297 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  NHU86_RS02210 (NHU86_02205) zapD 454308..455030 (-) 723 WP_010675825.1 cell division protein ZapD -
  NHU86_RS02215 (NHU86_02210) coaE 455068..455682 (-) 615 WP_113069550.1 dephospho-CoA kinase -
  NHU86_RS02220 (NHU86_02215) pilD 455701..456576 (-) 876 WP_113069551.1 A24 family peptidase Machinery gene
  NHU86_RS02225 (NHU86_02220) pilC 456654..457895 (-) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  NHU86_RS02230 (NHU86_02225) pilB 458023..459729 (-) 1707 WP_041213997.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NHU86_RS02235 (NHU86_02230) - 459733..460278 (-) 546 WP_113069552.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NHU86_RS02240 (NHU86_02235) nadC 460589..461452 (-) 864 WP_042864860.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NHU86_RS02245 (NHU86_02240) - 461456..461926 (-) 471 WP_104452204.1 TIGR02281 family clan AA aspartic protease -
  NHU86_RS02250 (NHU86_02245) ampD 462055..462627 (+) 573 WP_053288099.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45435.37 Da        Isoelectric Point: 9.9380

>NTDB_id=701437 NHU86_RS02225 WP_010675822.1 456654..457895(-) (pilC) [Aeromonas caviae strain OT55]
MATLAQKRNAPKKVFSYRWHGVNRKGQKVSGELQADSITTVKAELRKQGVNVTRVSKQSQGLFSKGGAKIKPMDIAVISR
QITTMLSAGVPLVQSLQIIARGHEKAAVRELIGQIAADVETGTPLSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
AIYREKNEALKSKIKKAMFYPSMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPAFTRFVIAISRFMQEWWYAIFGGAA
LAVFLYVRAWRKSQKVRDNTDKFILTIPVVGNILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTATMAIR
NEVVAGMQINVAMRTVDLFPDMVTQMVMIGEESGAIDDMLSKVAAIFEQEVDDMVDGLTSLLEPIIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=701437 NHU86_RS02225 WP_010675822.1 456654..457895(-) (pilC) [Aeromonas caviae strain OT55]
ATGGCCACATTAGCTCAAAAACGCAACGCACCGAAAAAAGTCTTCTCCTACCGCTGGCACGGCGTCAACCGCAAGGGACA
GAAGGTCTCCGGCGAGCTGCAAGCCGACAGCATCACCACCGTCAAGGCCGAGCTGCGCAAGCAGGGCGTCAACGTCACCA
GGGTCAGCAAGCAGAGCCAGGGGCTCTTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATCGCCGTCATCTCCCGC
CAGATCACTACCATGCTCTCCGCCGGCGTGCCTCTGGTGCAGAGTCTTCAGATCATTGCTCGCGGCCACGAGAAAGCGGC
GGTGCGCGAACTCATCGGCCAGATCGCCGCCGATGTGGAAACCGGCACCCCGCTCTCGGAGGCGTTGCGCCGCCATCCCC
GCCACTTCGACGACCTCTATTGCGACCTGGTCGAGGCCGGGGAGCAATCCGGTGCCCTGGAGACCATCTACGACCGTATC
GCCATCTACCGCGAGAAGAACGAAGCCCTCAAATCCAAGATCAAGAAGGCCATGTTCTATCCGTCCATGGTCATTCTGGT
GGCCATCGTCGTCACCTCCATCCTGCTGCTCTTCGTCATTCCCCAGTTCGAGGATATCTTCAAGAGCTTCGGTGCCGAGC
TGCCCGCCTTTACCCGGTTCGTCATCGCCATCTCCCGCTTTATGCAGGAGTGGTGGTACGCCATCTTTGGCGGCGCTGCT
CTCGCCGTCTTCCTCTATGTGCGGGCCTGGCGAAAATCCCAGAAGGTCAGGGACAACACGGACAAGTTCATCCTCACCAT
TCCCGTGGTGGGCAACATACTGCACAAGGCTGCCATGGCCCGTTTTGCCCGCACCCTCTCCACCACCTTCTCCGCCGGTA
TCCCACTGGTGGATGCCCTGGTCTCAGCGGCCGGAGCATCGGGCAACTATGTCTATCGCACTGCGACCATGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAGATCAACGTCGCCATGCGCACCGTGGATCTTTTCCCCGACATGGTGACCCAGATGGT
GATGATCGGTGAGGAATCCGGCGCCATCGACGACATGCTCTCCAAGGTCGCCGCCATTTTCGAACAGGAAGTGGACGACA
TGGTCGACGGCCTCACCAGCCTGCTCGAACCCATCATAATGGTGGTGCTCGGGGTGCTGGTCGGCGGCATGGTCGTGGCC
ATGTACCTCCCCATCTTCAAGCTGGGCTCTGTGATACACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TT63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.566

95.884

0.542
  pilC Legionella pneumophila strain ERS1305867

52.099

98.063

0.511
  pilC Acinetobacter baylyi ADP1

50.971

99.758

0.508
  pilC Acinetobacter baumannii D1279779

52.369

97.094

0.508
  pilC Vibrio cholerae strain A1552

47.368

96.61

0.458
  pilC Vibrio campbellii strain DS40M4

45.113

96.61

0.436
  pilG Neisseria meningitidis 44/76-A

41.602

93.705

0.39
  pilG Neisseria gonorrhoeae MS11

41.602

93.705

0.39
  pilC Thermus thermophilus HB27

38.75

96.852

0.375