Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NF680_RS22210 Genome accession   NZ_CP099602
Coordinates   4983648..4984865 (+) Length   405 a.a.
NCBI ID   WP_252876913.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6MONTID1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4978648..4989865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF680_RS22190 (NF680_22190) - 4980083..4980382 (+) 300 WP_252876911.1 DUF2845 domain-containing protein -
  NF680_RS22195 (NF680_22195) - 4980647..4981000 (-) 354 WP_071173629.1 BON domain-containing protein -
  NF680_RS22200 (NF680_22200) pilA/pilAI 4981296..4981718 (-) 423 WP_252876912.1 pilin Machinery gene
  NF680_RS22205 (NF680_22205) pilB 4981945..4983645 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF680_RS22210 (NF680_22210) pilC 4983648..4984865 (+) 1218 WP_252876913.1 type II secretion system F family protein Machinery gene
  NF680_RS22215 (NF680_22215) pilD 4984867..4985739 (+) 873 WP_252876914.1 A24 family peptidase Machinery gene
  NF680_RS22220 (NF680_22220) coaE 4985736..4986359 (+) 624 WP_252876915.1 dephospho-CoA kinase -
  NF680_RS22225 (NF680_22225) yacG 4986356..4986556 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  NF680_RS22230 (NF680_22230) - 4986564..4986779 (-) 216 WP_016773148.1 hypothetical protein -
  NF680_RS22235 (NF680_22235) - 4986845..4987534 (-) 690 WP_236168695.1 energy-coupling factor ABC transporter permease -
  NF680_RS22240 (NF680_22240) - 4987652..4988278 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  NF680_RS22245 (NF680_22245) - 4988275..4988802 (+) 528 WP_252876916.1 MOSC domain-containing protein -
  NF680_RS22250 (NF680_22250) - 4988854..4989027 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44085.80 Da        Isoelectric Point: 10.1214

>NTDB_id=701023 NF680_RS22210 WP_252876913.1 4983648..4984865(+) (pilC) [Pseudomonas siliginis strain OTU6MONTID1]
MAVKAAKVSIYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLTFGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAVFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=701023 NF680_RS22210 WP_252876913.1 4983648..4984865(+) (pilC) [Pseudomonas siliginis strain OTU6MONTID1]
ATGGCGGTCAAGGCAGCGAAAGTCAGCATTTACGCCTGGGAAGGCACGGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGACAGAACCCCGCACTGATCAAGGCGCAACTGCGCAAACAGGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CGTCCCTGCTGACCTTCGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCAACGATGATGAAG
GCCGGTGTACCGCTGTTGCAGTCCTTCGACATCATCGGCGAAGGCTTCGAAAATCCGGCCATGCGCAAACTGGTCGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCTACGGCCCTGCGCAAGAAACCACAATACTTCGACGAGTTGTACT
GCAATCTGGTCGACGCCGGCGAACAGTCTGGCGCCCTCGACACCTTGCTTGAACGTGTGGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAAAAAGCCATGACCTATCCCACCGCCGTGGTGCTGGTGGCGGCAGTGGTAACAGGCAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAGTCGGTGTTTTCCGGGTTCGGCGCCGAACTGCCGGGCTTCACCCTGATGA
TCATCAGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCCATTCTTTTTGCGCTGGTGGCGGTGTTTTTCGGCACTCGC
CACGCGCTGAAGACATCGCAGGCCCTGCGTGACCGCCGCGATGCCTGGCTGTTGAAGCTGCCACTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTCGCACGTTTTGCCCGCACCCTGTCGACCACATTCGCAGCCGGTGTGCCGCTGGTGGAAGCGCTGG
ATTCGGTCGCCGGTGCCACCGGCAATGTGGTGTTCAAGCGCGCGGTGTTGCGGGTGCGGCAGGACGTCTCGACCGGCATG
CAGCTCAATTTCTCAATGCGCAGCACCGGTGTGTTTCCGAACATGGCAGTGCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baumannii D1279779

58.824

100

0.593
  pilC Acinetobacter baylyi ADP1

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

53

98.765

0.523
  pilC Vibrio cholerae strain A1552

44.444

97.778

0.435
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.872

100

0.42
  pilC Thermus thermophilus HB27

39.348

98.519

0.388