Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NF676_RS23030 Genome accession   NZ_CP099598
Coordinates   5022951..5024168 (+) Length   405 a.a.
NCBI ID   WP_095181835.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6BANIB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5017951..5029168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF676_RS23010 (NF676_23015) - 5019394..5019693 (+) 300 WP_039761633.1 DUF2845 domain-containing protein -
  NF676_RS23015 (NF676_23020) - 5019957..5020310 (-) 354 WP_071173629.1 BON domain-containing protein -
  NF676_RS23020 (NF676_23025) - 5020605..5021021 (-) 417 WP_056790436.1 pilin -
  NF676_RS23025 (NF676_23030) pilB 5021248..5022948 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF676_RS23030 (NF676_23035) pilC 5022951..5024168 (+) 1218 WP_095181835.1 type II secretion system F family protein Machinery gene
  NF676_RS23035 (NF676_23040) pilD 5024170..5025042 (+) 873 WP_041477817.1 A24 family peptidase Machinery gene
  NF676_RS23040 (NF676_23045) coaE 5025039..5025662 (+) 624 WP_110646726.1 dephospho-CoA kinase -
  NF676_RS23045 (NF676_23050) yacG 5025659..5025859 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  NF676_RS23050 (NF676_23055) - 5025867..5026082 (-) 216 WP_016773148.1 hypothetical protein -
  NF676_RS23055 (NF676_23060) - 5026148..5026837 (-) 690 WP_041477814.1 energy-coupling factor ABC transporter permease -
  NF676_RS23060 (NF676_23065) - 5027028..5027654 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  NF676_RS23065 (NF676_23070) - 5027651..5028178 (+) 528 WP_160768590.1 MOSC domain-containing protein -
  NF676_RS23070 (NF676_23075) - 5028230..5028403 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44009.70 Da        Isoelectric Point: 10.1214

>NTDB_id=700905 NF676_RS23030 WP_095181835.1 5022951..5024168(+) (pilC) [Pseudomonas siliginis strain OTU6BANIB1]
MAVKAAKVSVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLTLGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAAFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=700905 NF676_RS23030 WP_095181835.1 5022951..5024168(+) (pilC) [Pseudomonas siliginis strain OTU6BANIB1]
ATGGCGGTCAAGGCAGCGAAAGTCAGCGTTTACGCCTGGGAAGGCACGGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGACAGAACCCCGCACTGATCAAGGCGCAACTGCGCAAACAGGGGATAAACCCGGGCAAGGTGCGCAAGAAATCCG
CCTCCCTGCTGACCTTGGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCCTTCGACATCATCGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAACTGGTCGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCTACGGCCCTGCGCAAGAAACCGCAATACTTCGACGAGTTGTACT
GCAATCTGGTCGACGCCGGCGAACAGTCTGGCGCCCTCGACACCTTGCTTGAACGGGTCGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCCACCGCCGTGGTGCTGGTGGCGGCAGTGGTAACAGGCAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAGTCGGTGTTTTCCGGGTTCGGCGCAGAACTGCCGGGCTTCACCCTGATGA
TCATCAGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCCATTCTTTTTGCGCTGGTGGCAGCGTTTTTCGGCACTCGC
CACGCGTTGAAGACTTCGCAGGCCCTGCGCGACCGGCGCGATGCCTGGCTGCTGAAGCTGCCACTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTCGCACGTTTTGCCCGCACCCTGTCGACCACATTCGCAGCCGGTGTGCCGCTGGTGGAAGCACTGG
ATTCGGTCGCCGGTGCCACCGGCAATGTGGTGTTCAAGCGCGCGGTGTTGCGGGTGCGGCAGGACGTCTCGACCGGCATG
CAACTGAATTTCTCCATGCGCAGCACCGGTGTGTTTCCCAACATGGCGGTGCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAG
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

52.75

98.765

0.521
  pilC Vibrio cholerae strain A1552

44.949

97.778

0.44
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.872

100

0.42
  pilC Thermus thermophilus HB27

39.85

98.519

0.393