Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NF676_RS23025 Genome accession   NZ_CP099598
Coordinates   5021248..5022948 (+) Length   566 a.a.
NCBI ID   WP_056790439.1    Uniprot ID   A0AAQ3G636
Organism   Pseudomonas siliginis strain OTU6BANIB1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5016248..5027948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF676_RS23005 (NF676_23010) - 5016455..5019397 (+) 2943 WP_252886815.1 DUF748 domain-containing protein -
  NF676_RS23010 (NF676_23015) - 5019394..5019693 (+) 300 WP_039761633.1 DUF2845 domain-containing protein -
  NF676_RS23015 (NF676_23020) - 5019957..5020310 (-) 354 WP_071173629.1 BON domain-containing protein -
  NF676_RS23020 (NF676_23025) - 5020605..5021021 (-) 417 WP_056790436.1 pilin -
  NF676_RS23025 (NF676_23030) pilB 5021248..5022948 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF676_RS23030 (NF676_23035) pilC 5022951..5024168 (+) 1218 WP_095181835.1 type II secretion system F family protein Machinery gene
  NF676_RS23035 (NF676_23040) pilD 5024170..5025042 (+) 873 WP_041477817.1 A24 family peptidase Machinery gene
  NF676_RS23040 (NF676_23045) coaE 5025039..5025662 (+) 624 WP_110646726.1 dephospho-CoA kinase -
  NF676_RS23045 (NF676_23050) yacG 5025659..5025859 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  NF676_RS23050 (NF676_23055) - 5025867..5026082 (-) 216 WP_016773148.1 hypothetical protein -
  NF676_RS23055 (NF676_23060) - 5026148..5026837 (-) 690 WP_041477814.1 energy-coupling factor ABC transporter permease -
  NF676_RS23060 (NF676_23065) - 5027028..5027654 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62620.67 Da        Isoelectric Point: 6.1319

>NTDB_id=700904 NF676_RS23025 WP_056790439.1 5021248..5022948(+) (pilB) [Pseudomonas siliginis strain OTU6BANIB1]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLHCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMADVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=700904 NF676_RS23025 WP_056790439.1 5021248..5022948(+) (pilB) [Pseudomonas siliginis strain OTU6BANIB1]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAACAAGCGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGCGTGTCTCTGGTCAATTACCTGGTGCACAACAAACTGGTCAACAGCCGGCAAATTG
CCGAGATCGCCTCGGAACATTTCGGCATGGCCCTGCTCGATCTGCATTGCCTCGACAAAGAGACTCAGCCCAAGGGTCTG
GTCAGTGAGAAACTGGTTCGCCAGCACTGCGCCCTGCCCCTCTGGCGGCGCGGCAACAAACTGTTCGTGGGCCTTTCCGA
CCCAAGCAATCAGCAGGCGATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGACAAGTTCTTCGACAGCCATGCCTCGGGCCTGGAGGAAATGGCCGATGTCGATCTCGACGGC
CTCGACGTCGAGTCCATTGATGACAACAAACAGGACGTGATCGGCGGCCTCGATGCCGACGACGCCCCGGTCGTACGTTT
TGTGCACAAGATGCTGCTCGATGCGATCAAAAGCGGCTCTTCCGACCTGCATTTCGAACCCTACGAAAAAAACTATCGGG
TGCGCATGCGCACCGACGGCATGCTGCGCGAGGTGGCCAAGCCGCCGATCCAGTTGGCCGGGCGCATTGCCGCGCGCTTG
AAGGTAATGGCCAGTCTGGATATCTCGGAACGGCGCAAACCTCAGGACGGCCGCATCAAAATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTTCGCGTGAACACCCTGCCGACTCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTG
CACAAATGGGCATCGATGCGCTGGGCTACGAGGCCGAACAAAAAGCCTTGTACCTGGCCGCGCTGAAACAGCCGCAAGGG
ATGATTCTGGTCACCGGCCCCACCGGCTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
CATCAATATTTCCACCGCCGAAGATCCGGTGGAAATCAACATGGAAGGTATCAATCAGGTCAACGTCAATCCCCGCCAAG
GGCTGGACTTTGCCCAAGCGCTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCAATCAAGGCTGCGCAGACCGGCCACCTGGTGCTGTCGACCCTGCACACCAATAGCGCAGCGGA
AACCCTGACACGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCCCAGCGTC
TGGCGCGCAAGTTGTGCGCACACTGCAAGCGGCCGCTGGAGATCCCGCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAATGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAGAACACACCGGACCTGCAACGGTTGATCATGGCCGAGGGCAATTCGCTGGAAATCGATATTCAGA
TGCGCCGCGACGGCTTCGCCGATCTGCGCACTTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.446

99.117

0.5
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.209

100

0.495
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.837

94.17

0.366