Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PCP19_RS01590 Genome accession   NZ_CP115215
Coordinates   353589..354053 (-) Length   154 a.a.
NCBI ID   WP_329006229.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F062     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 348589..359053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP19_RS01575 (PCP19_01570) nadC 351089..351937 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PCP19_RS01585 (PCP19_01580) - 352118..353431 (-) 1314 WP_023090841.1 O-antigen ligase family protein -
  PCP19_RS01590 (PCP19_01585) pilA/pilAI 353589..354053 (-) 465 WP_329006229.1 pilin Machinery gene
  PCP19_RS01595 (PCP19_01590) pilB 354284..355984 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP19_RS01600 (PCP19_01595) - 355988..357205 (+) 1218 Protein_312 type II secretion system F family protein -
  PCP19_RS01605 (PCP19_01600) pilD 357206..358078 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP19_RS01610 (PCP19_01605) coaE 358075..358686 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PCP19_RS01615 (PCP19_01610) yacG 358683..358883 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16035.35 Da        Isoelectric Point: 9.0066

>NTDB_id=696195 PCP19_RS01590 WP_329006229.1 353589..354053(-) (pilA/pilAI) [Pseudomonas aeruginosa strain F062]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSSDTPGNNEYDLGFTS
STLLTGSGKGQIKIDKADTATPEISGILGNSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKTNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=696195 PCP19_RS01590 WP_329006229.1 353589..354053(-) (pilA/pilAI) [Pseudomonas aeruginosa strain F062]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAGCGATACCCCCGGAAACAATGAATATGATCTTGGCTTTACCAGC
TCTACTCTGCTTACTGGTAGCGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAT
TCTGGGCAACTCTTCTGGTAAGGGTATCGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAACCAACTACGCCCCGGCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Acinetobacter baumannii strain A118

44.138

94.156

0.416
  pilA Pseudomonas aeruginosa PAK

40.506

100

0.416
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.789

98.701

0.403
  pilA Vibrio cholerae strain A1552

40.789

98.701

0.403
  pilA Vibrio cholerae C6706

40.789

98.701

0.403
  pilA Ralstonia pseudosolanacearum GMI1000

35.294

100

0.39
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.268

96.753

0.39