Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NAL94_RS09520 Genome accession   NZ_CP098034
Coordinates   335214..336959 (+) Length   581 a.a.
NCBI ID   WP_206225429.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain E110     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330214..341959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAL94_RS09500 (NAL94_09500) pilM 332522..333538 (+) 1017 WP_115386336.1 type IV pilus biogenesis protein PilM Machinery gene
  NAL94_RS09505 (NAL94_09505) pilN 333522..334097 (+) 576 WP_115386335.1 PilN domain-containing protein Machinery gene
  NAL94_RS09510 (NAL94_09510) pilO 334090..334683 (+) 594 WP_005382767.1 type 4a pilus biogenesis protein PilO Machinery gene
  NAL94_RS09515 (NAL94_09515) pilP 334673..335188 (+) 516 WP_206225430.1 pilus assembly protein PilP Machinery gene
  NAL94_RS09520 (NAL94_09520) pilQ 335214..336959 (+) 1746 WP_206225429.1 type IV pilus secretin PilQ Machinery gene
  NAL94_RS09525 (NAL94_09525) aroK 337147..337665 (+) 519 WP_005381319.1 shikimate kinase AroK -
  NAL94_RS09530 (NAL94_09530) aroB 337697..338797 (+) 1101 WP_005381318.1 3-dehydroquinate synthase -
  NAL94_RS09535 (NAL94_09535) - 338850..340361 (+) 1512 WP_025768079.1 SPOR domain-containing protein -
  NAL94_RS09540 (NAL94_09540) - 340431..341270 (+) 840 WP_005382762.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63679.67 Da        Isoelectric Point: 4.4593

>NTDB_id=693677 NAL94_RS09520 WP_206225429.1 335214..336959(+) (pilQ) [Vibrio alginolyticus strain E110]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTAVESVEVFRDTSTTRLVAHINEEYMHDYRLTGRFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGIDDFLNVNLAATSPNAT
SIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=693677 NAL94_RS09520 WP_206225429.1 335214..336959(+) (pilQ) [Vibrio alginolyticus strain E110]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCGG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGCGGTTGAGAGCGTAGAAGTGTT
TCGCGACACCTCGACGACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGCTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTTACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGAGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGGATTCGTTGGGGTTTTACGTC
TATTAATGGCAGTAATACAGTGGGCGGTTCTATTGAGAACAACCTCGCCACGATCGGTCTCTATAATGGAGAAGGGGGTG
ATGACGGTGGTGACGGTGATGGAGTAGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACCAGTCCGAATGCCACC
AGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCTCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCTGCCTATATCGAACAAGGTACAGAGATTCCTT
ATCTTGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTAACCCCGCAAATC
ACACCGGATAACCGCTTAGTATTGGATTTAAGTGTCACGCAAGATAGACCTGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGATCTTCCGCTATTAGGTGCGCTTTTTCGTCGA
AGTTATGAAAATGTTGGTAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.771

100

0.928
  pilQ Vibrio cholerae strain A1552

74.742

100

0.749
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.742

100

0.749
  pilQ Pseudomonas aeruginosa PAK

39.896

99.656

0.398
  pilQ Legionella pneumophila strain Lp02

40.608

96.213

0.391
  pilQ Legionella pneumophila strain ERS1305867

40.608

96.213

0.391
  comQ Acinetobacter baylyi ADP1

37.414

99.828

0.373
  pilQ Acinetobacter baumannii strain A118

35.906

100

0.368
  pilQ Acinetobacter baumannii D1279779

35.906

100

0.368