Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NAL94_RS09500 Genome accession   NZ_CP098034
Coordinates   332522..333538 (+) Length   338 a.a.
NCBI ID   WP_115386336.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain E110     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327522..338538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAL94_RS09485 (NAL94_09485) - 327953..328681 (-) 729 WP_005382781.1 redoxin family protein -
  NAL94_RS09490 (NAL94_09490) oxyR 328851..329762 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  NAL94_RS09495 (NAL94_09495) - 329816..332377 (-) 2562 WP_206225431.1 penicillin-binding protein 1A -
  NAL94_RS09500 (NAL94_09500) pilM 332522..333538 (+) 1017 WP_115386336.1 type IV pilus biogenesis protein PilM Machinery gene
  NAL94_RS09505 (NAL94_09505) pilN 333522..334097 (+) 576 WP_115386335.1 PilN domain-containing protein Machinery gene
  NAL94_RS09510 (NAL94_09510) pilO 334090..334683 (+) 594 WP_005382767.1 type 4a pilus biogenesis protein PilO Machinery gene
  NAL94_RS09515 (NAL94_09515) pilP 334673..335188 (+) 516 WP_206225430.1 pilus assembly protein PilP Machinery gene
  NAL94_RS09520 (NAL94_09520) pilQ 335214..336959 (+) 1746 WP_206225429.1 type IV pilus secretin PilQ Machinery gene
  NAL94_RS09525 (NAL94_09525) aroK 337147..337665 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37626.03 Da        Isoelectric Point: 7.2090

>NTDB_id=693673 NAL94_RS09500 WP_115386336.1 332522..333538(+) (pilM) [Vibrio alginolyticus strain E110]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGVNCASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=693673 NAL94_RS09500 WP_115386336.1 332522..333538(+) (pilM) [Vibrio alginolyticus strain E110]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGTAAACTGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.84

100

0.828
  pilM Vibrio cholerae strain A1552

50.448

99.112

0.5