Detailed information    

insolico Bioinformatically predicted

Overview

Name   comP   Type   Machinery gene
Locus tag   CKCBHOJB_RS00280 Genome accession   NZ_CP097870
Coordinates   63471..63884 (+) Length   137 a.a.
NCBI ID   WP_281050087.1    Uniprot ID   -
Organism   Thauera sp. GDN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58471..68884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKCBHOJB_RS00255 (CKCBHOJB_00051) - 59593..59811 (+) 219 WP_281050082.1 glycine zipper 2TM domain-containing protein -
  CKCBHOJB_RS00260 (CKCBHOJB_00052) - 59911..60471 (-) 561 WP_281050083.1 mechanosensitive ion channel family protein -
  CKCBHOJB_RS00265 (CKCBHOJB_00053) - 60633..62240 (+) 1608 WP_281050084.1 peptide chain release factor 3 -
  CKCBHOJB_RS00270 (CKCBHOJB_00054) - 62272..62406 (+) 135 WP_281050085.1 hypothetical protein -
  CKCBHOJB_RS00275 (CKCBHOJB_00055) - 62599..63183 (-) 585 WP_281050086.1 hypothetical protein -
  CKCBHOJB_RS00280 comP 63471..63884 (+) 414 WP_281050087.1 pilin Machinery gene
  CKCBHOJB_RS00285 (CKCBHOJB_00056) - 64154..65539 (+) 1386 WP_281050088.1 sulfatase-like hydrolase/transferase -
  CKCBHOJB_RS00290 (CKCBHOJB_00057) - 65545..66759 (+) 1215 WP_281050089.1 O-antigen ligase family protein -
  CKCBHOJB_RS00295 (CKCBHOJB_00058) - 66805..66999 (-) 195 WP_281050090.1 hypothetical protein -
  CKCBHOJB_RS00300 (CKCBHOJB_00059) hemG 67006..67527 (-) 522 WP_281050091.1 menaquinone-dependent protoporphyrinogen IX dehydrogenase -
  CKCBHOJB_RS00305 (CKCBHOJB_00060) - 67561..67941 (-) 381 WP_281050092.1 hypothetical protein -
  CKCBHOJB_RS00310 (CKCBHOJB_00061) - 67978..68847 (-) 870 WP_281050093.1 SPFH domain-containing protein -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14141.29 Da        Isoelectric Point: 4.4091

>NTDB_id=693161 CKCBHOJB_RS00280 WP_281050087.1 63471..63884(+) (comP) [Thauera sp. GDN1]
MKKMQQGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVSEGLVQASAAKVTVGENIASDVTDFCLGVSVGENGRSTLA
CEDGGILTISVDAGAKAPVNLTLAPTPGDAGVVWRCTVDDLANQAKYVPAECRAATS

Nucleotide


Download         Length: 414 bp        

>NTDB_id=693161 CKCBHOJB_RS00280 WP_281050087.1 63471..63884(+) (comP) [Thauera sp. GDN1]
ATGAAAAAGATGCAACAGGGCTTCACGCTGATCGAACTGATGATCGTCGTGGCGATTATTGGCATTCTCGCCGCGGTGGC
GCTGCCAGCGTATCAGGATTACACGATTCGGGCACGTGTTAGTGAGGGCTTGGTTCAAGCTTCGGCAGCGAAAGTAACCG
TTGGGGAGAATATTGCATCGGACGTGACAGATTTTTGCCTCGGCGTAAGCGTCGGAGAAAACGGTAGATCAACACTAGCG
TGTGAAGATGGCGGCATTCTGACGATTTCGGTTGATGCTGGCGCTAAAGCTCCCGTGAATCTCACCTTGGCTCCAACACC
TGGCGACGCCGGTGTCGTTTGGCGCTGTACGGTCGATGATCTCGCCAATCAGGCGAAATATGTCCCTGCGGAATGCCGTG
CCGCAACGAGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Acinetobacter baylyi ADP1

45.89

100

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

40

100

0.482
  pilA2 Legionella pneumophila str. Paris

46.043

100

0.467
  pilA2 Legionella pneumophila strain ERS1305867

44.928

100

0.453
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.559

100

0.423
  pilA Pseudomonas aeruginosa PAK

36.842

100

0.409
  pilA Vibrio parahaemolyticus RIMD 2210633

46.957

83.942

0.394
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.111

100

0.38
  pilA/pilA1 Eikenella corrodens VA1

40.65

89.781

0.365