Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   C820_RS08995 Genome accession   NZ_CP097810
Coordinates   1793617..1793994 (+) Length   125 a.a.
NCBI ID   WP_004034628.1    Uniprot ID   N1ZLD0
Organism   Clostridium sp. MD294 strain ASF356     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1779178..1820459 1793617..1793994 within 0


Gene organization within MGE regions


Location: 1779178..1820459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C820_RS08835 (C820_001758) - 1779178..1780356 (-) 1179 WP_004034665.1 tyrosine-type recombinase/integrase -
  C820_RS08840 (C820_001759) - 1780503..1781342 (-) 840 WP_004034664.1 Rpn family recombination-promoting nuclease/putative transposase -
  C820_RS08845 (C820_001760) - 1781422..1782411 (-) 990 WP_004034663.1 replication protein -
  C820_RS08850 (C820_001761) - 1782746..1783222 (-) 477 WP_004034662.1 helix-turn-helix domain-containing protein -
  C820_RS08855 (C820_001762) - 1783395..1783601 (+) 207 WP_004034661.1 helix-turn-helix transcriptional regulator -
  C820_RS08860 (C820_001763) - 1783670..1783879 (-) 210 WP_004034660.1 type II toxin-antitoxin system HicB family antitoxin -
  C820_RS08865 (C820_001764) - 1783872..1784132 (-) 261 WP_004034659.1 hypothetical protein -
  C820_RS08870 (C820_001765) - 1784161..1784388 (-) 228 WP_004034658.1 hypothetical protein -
  C820_RS08875 (C820_001766) - 1784633..1784851 (-) 219 WP_004034657.1 helix-turn-helix transcriptional regulator -
  C820_RS08880 (C820_001767) - 1784994..1785182 (+) 189 WP_004034656.1 hypothetical protein -
  C820_RS08885 (C820_001768) - 1785179..1785400 (+) 222 WP_004034655.1 helix-turn-helix transcriptional regulator -
  C820_RS08890 (C820_001769) - 1785442..1785612 (+) 171 WP_004034654.1 hypothetical protein -
  C820_RS08895 - 1785578..1785961 (-) 384 WP_083863683.1 DUF2513 domain-containing protein -
  C820_RS08900 (C820_001770) - 1786139..1786321 (+) 183 WP_004034653.1 helix-turn-helix transcriptional regulator -
  C820_RS08905 (C820_001771) - 1786405..1786818 (+) 414 WP_004034652.1 hypothetical protein -
  C820_RS08910 (C820_001772) - 1787022..1787249 (+) 228 WP_004034651.1 hypothetical protein -
  C820_RS08915 (C820_001773) - 1787343..1787615 (+) 273 WP_004034650.1 Veg family protein -
  C820_RS08920 (C820_001774) - 1787739..1788020 (+) 282 WP_004034649.1 hypothetical protein -
  C820_RS08925 (C820_001775) - 1788017..1788310 (+) 294 WP_004034648.1 Rha family transcriptional regulator -
  C820_RS08930 (C820_001776) - 1788320..1788694 (+) 375 WP_004034647.1 hypothetical protein -
  C820_RS08935 (C820_001777) - 1788747..1789010 (-) 264 WP_004034646.1 Txe/YoeB family addiction module toxin -
  C820_RS08940 (C820_001778) - 1789007..1789270 (-) 264 WP_004034644.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
  C820_RS08945 (C820_001779) - 1789351..1789581 (-) 231 WP_004034642.1 helix-turn-helix transcriptional regulator -
  C820_RS14475 - 1789723..1789848 (+) 126 WP_279282464.1 hypothetical protein -
  C820_RS08950 (C820_001781) - 1790040..1790237 (+) 198 WP_004034639.1 helix-turn-helix transcriptional regulator -
  C820_RS08955 (C820_001782) - 1790296..1790526 (-) 231 WP_004034638.1 helix-turn-helix transcriptional regulator -
  C820_RS08960 (C820_001783) - 1790811..1791050 (-) 240 WP_004034637.1 helix-turn-helix transcriptional regulator -
  C820_RS14480 - 1791212..1791334 (+) 123 WP_279282463.1 hypothetical protein -
  C820_RS08965 (C820_001784) - 1791527..1791754 (+) 228 WP_004034634.1 helix-turn-helix transcriptional regulator -
  C820_RS08970 (C820_001785) - 1791767..1791952 (+) 186 WP_156801391.1 hypothetical protein -
  C820_RS08975 (C820_001786) - 1791949..1792125 (+) 177 WP_004034632.1 hypothetical protein -
  C820_RS08980 (C820_001787) - 1792212..1792712 (+) 501 WP_004034631.1 siphovirus Gp157 family protein -
  C820_RS08985 (C820_001788) - 1792727..1793071 (+) 345 WP_004034630.1 hypothetical protein -
  C820_RS08990 (C820_001789) - 1793046..1793624 (+) 579 WP_004034629.1 ERF family protein -
  C820_RS08995 (C820_001790) ssbA 1793617..1793994 (+) 378 WP_004034628.1 single-stranded DNA-binding protein Machinery gene
  C820_RS09000 (C820_001791) - 1794136..1795011 (+) 876 WP_004034627.1 hypothetical protein -
  C820_RS09005 (C820_001792) - 1795008..1795703 (+) 696 WP_004034624.1 replicative helicase loader/inhibitor -
  C820_RS09010 (C820_001793) - 1795887..1796159 (+) 273 WP_004034623.1 hypothetical protein -
  C820_RS09015 (C820_001794) - 1796336..1796749 (+) 414 WP_004034622.1 hypothetical protein -
  C820_RS09020 (C820_001795) - 1797007..1797660 (+) 654 WP_004034621.1 helix-turn-helix domain-containing protein -
  C820_RS09025 (C820_001796) terL 1797662..1799092 (+) 1431 WP_004034619.1 phage terminase large subunit -
  C820_RS09030 (C820_001797) - 1799114..1800376 (+) 1263 WP_251855591.1 DUF935 domain-containing protein -
  C820_RS09035 (C820_001798) - 1800380..1801918 (+) 1539 WP_004034615.1 phage minor head protein -
  C820_RS09040 (C820_001799) - 1801915..1802184 (+) 270 WP_004034613.1 hypothetical protein -
  C820_RS09045 (C820_001800) - 1802347..1802982 (+) 636 WP_004034611.1 hypothetical protein -
  C820_RS09050 (C820_001801) - 1802986..1803228 (+) 243 WP_004034610.1 hypothetical protein -
  C820_RS09055 (C820_001803) - 1803492..1803674 (+) 183 WP_004034608.1 hypothetical protein -
  C820_RS09060 (C820_001804) - 1803763..1804203 (+) 441 WP_004034607.1 XkdF-like putative serine protease domain-containing protein -
  C820_RS14485 (C820_001805) - 1804283..1804405 (+) 123 WP_004034606.1 hypothetical protein -
  C820_RS09065 (C820_001806) - 1804600..1805493 (+) 894 WP_004034605.1 hypothetical protein -
  C820_RS09070 (C820_001807) - 1805543..1806220 (+) 678 WP_004034604.1 LysM peptidoglycan-binding domain-containing protein -
  C820_RS09075 (C820_001808) - 1806234..1807217 (+) 984 WP_004034603.1 hypothetical protein -
  C820_RS09080 (C820_001809) - 1807214..1807720 (+) 507 WP_004034602.1 hypothetical protein -
  C820_RS09085 (C820_001810) - 1807713..1808162 (+) 450 WP_004034601.1 DUF2634 domain-containing protein -
  C820_RS09090 (C820_001811) - 1808232..1808402 (+) 171 WP_004034600.1 type II toxin-antitoxin system HicA family toxin -
  C820_RS09095 (C820_001812) - 1808457..1808870 (+) 414 WP_004034599.1 type II toxin-antitoxin system HicB family antitoxin -
  C820_RS09100 (C820_001813) - 1808936..1809352 (+) 417 WP_004034598.1 type II toxin-antitoxin system HicB family antitoxin -
  C820_RS09105 (C820_001814) - 1809410..1810528 (+) 1119 WP_004034597.1 baseplate J/gp47 family protein -
  C820_RS09110 (C820_001815) - 1810525..1811265 (+) 741 WP_004034596.1 putative phage tail protein -
  C820_RS09115 (C820_001816) - 1811773..1813665 (+) 1893 WP_162213044.1 hypothetical protein -
  C820_RS09120 (C820_001817) - 1813628..1815091 (+) 1464 WP_004034594.1 peptidase G2 autoproteolytic cleavage domain-containing protein -
  C820_RS09125 (C820_001818) - 1815104..1815865 (+) 762 WP_004034593.1 hypothetical protein -
  C820_RS09130 (C820_001819) - 1815920..1816138 (-) 219 WP_004034592.1 helix-turn-helix transcriptional regulator -
  C820_RS09135 (C820_001820) - 1816131..1816394 (-) 264 WP_004034591.1 DUF2442 domain-containing protein -
  C820_RS09140 (C820_001821) - 1816438..1816698 (-) 261 WP_004034590.1 DUF4160 domain-containing protein -
  C820_RS09145 (C820_001822) - 1816873..1818657 (+) 1785 WP_004034589.1 fibronectin type III domain-containing protein -
  C820_RS09150 (C820_001823) - 1818654..1818842 (+) 189 WP_004034588.1 hypothetical protein -
  C820_RS09155 (C820_001824) - 1818835..1818987 (+) 153 WP_004034587.1 XkdX family protein -
  C820_RS09160 (C820_001825) - 1819088..1819342 (+) 255 WP_004034586.1 hypothetical protein -
  C820_RS09165 (C820_001826) - 1819344..1819607 (+) 264 WP_004034585.1 phage holin -
  C820_RS09170 (C820_001827) - 1819617..1820459 (+) 843 WP_004034584.1 peptidoglycan recognition family protein -

Sequence


Protein


Download         Length: 125 a.a.        Molecular weight: 14375.19 Da        Isoelectric Point: 7.9488

>NTDB_id=692783 C820_RS08995 WP_004034628.1 1793617..1793994(+) (ssbA) [Clostridium sp. MD294 strain ASF356]
MNKVILMGRLTKKPEVKYTQQNVAVARYTLAVARRFQQKGQSEVDFINCITFGKSAEFAQKYLNKGKQIAIVGKIQVRSW
ENENSQKQWSTEVIVEEQYFADSKVNETEGNSFNTVEQSDDDLPF

Nucleotide


Download         Length: 378 bp        

>NTDB_id=692783 C820_RS08995 WP_004034628.1 1793617..1793994(+) (ssbA) [Clostridium sp. MD294 strain ASF356]
ATGAATAAAGTGATATTGATGGGAAGATTGACAAAAAAACCAGAAGTAAAATATACACAGCAAAATGTAGCAGTTGCAAG
GTATACCCTTGCAGTTGCTAGAAGATTTCAGCAAAAAGGACAATCAGAAGTAGATTTTATAAATTGTATTACTTTTGGAA
AATCAGCAGAGTTTGCACAAAAGTATCTAAATAAAGGCAAACAGATAGCAATTGTAGGAAAAATACAAGTGCGTTCGTGG
GAAAATGAAAATAGTCAAAAGCAATGGAGTACAGAGGTGATTGTAGAAGAACAGTACTTTGCAGATAGTAAAGTAAATGA
AACAGAAGGAAATAGTTTTAATACAGTAGAACAAAGTGATGATGATTTACCATTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB N1ZLD0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

35.673

100

0.488

  ssbB/cilA Streptococcus pneumoniae TIGR4

36.154

100

0.376

  ssbB/cilA Streptococcus mitis NCTC 12261

36.154

100

0.376

  ssbB Streptococcus sobrinus strain NIDR 6715-7

36.154

100

0.376

  ssbB/cilA Streptococcus mitis SK321

35.385

100

0.368

  ssbA Streptococcus mutans UA159

35.385

100

0.368

  ssbB/cilA Streptococcus pneumoniae Rx1

35.385

100

0.368

  ssbB/cilA Streptococcus pneumoniae D39

35.385

100

0.368

  ssbB/cilA Streptococcus pneumoniae R6

35.385

100

0.368

  ssbB Bacillus subtilis subsp. subtilis str. 168

42.991

85.6

0.368