Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MW290_RS28285 Genome accession   NZ_CP097636
Coordinates   3020974..3022194 (+) Length   406 a.a.
NCBI ID   WP_250197689.1    Uniprot ID   -
Organism   Aquincola tertiaricarbonis strain RN12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3015974..3027194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW290_RS28255 (MW290_28250) cgtA 3016043..3017113 (-) 1071 WP_250197686.1 Obg family GTPase CgtA -
  MW290_RS28260 (MW290_28255) rpmA 3017201..3017458 (-) 258 WP_250197687.1 50S ribosomal protein L27 -
  MW290_RS28265 (MW290_28260) rplU 3017488..3017799 (-) 312 WP_250197688.1 50S ribosomal protein L21 -
  MW290_RS28270 (MW290_28265) ispB 3018030..3018971 (+) 942 WP_250200121.1 octaprenyl diphosphate synthase -
  MW290_RS28280 (MW290_28275) pilF 3019229..3020953 (+) 1725 WP_250200122.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MW290_RS28285 (MW290_28280) pilC 3020974..3022194 (+) 1221 WP_250197689.1 type II secretion system F family protein Machinery gene
  MW290_RS28290 (MW290_28285) - 3022202..3023134 (+) 933 WP_250200123.1 A24 family peptidase -
  MW290_RS28295 (MW290_28290) coaE 3023142..3023756 (+) 615 WP_250197690.1 dephospho-CoA kinase -
  MW290_RS28300 (MW290_28295) zapD 3023784..3024539 (+) 756 WP_250197691.1 cell division protein ZapD -
  MW290_RS28305 (MW290_28300) - 3024536..3024745 (+) 210 WP_250197692.1 DNA gyrase inhibitor YacG -
  MW290_RS28310 (MW290_28305) rpoN 3024827..3026263 (-) 1437 WP_250197693.1 RNA polymerase factor sigma-54 -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44202.02 Da        Isoelectric Point: 9.7575

>NTDB_id=691375 MW290_RS28285 WP_250197689.1 3020974..3022194(+) (pilC) [Aquincola tertiaricarbonis strain RN12]
MATAAAAKNIKEFVFEWEGKDKNGKTVRGEMRSGGEAAVSATLRRQGVMITKVKKRRMSGGKAIKQKDIAIFTRQLATMM
KAGVPLLQAFDIVGRGSSNPKLTRLLNDVRSDVETGTSLSAAFRKHPLYFNALYANLVEAGEAGGILEQLLERLALYEEK
TLALKSKIKSALMYPVAVMVVAFIVLTVIMLFVIPAFKDVFSSFGADLPAPTLAVIAMSEFFVSYWWLIFGVMFGGGYFF
MQSWRRSEKMQMAMDRLLLRIPVFGDLINKSVIARWTRTLSTMFAAGVPLVEALDSVGGAAGNAVYAEATEKIQKDVSTG
SALTNAMQATGVFPTMVLQMASIGEESGSLDHMLGKAAEFYEDEVDEMVKGLSSLMEPFIIVILGTLIGGIVVSMYLPIF
KLGSVV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=691375 MW290_RS28285 WP_250197689.1 3020974..3022194(+) (pilC) [Aquincola tertiaricarbonis strain RN12]
ATGGCGACTGCTGCGGCCGCGAAGAACATCAAAGAGTTCGTCTTCGAGTGGGAAGGCAAGGACAAGAACGGCAAGACCGT
GCGTGGTGAGATGCGCTCGGGCGGCGAGGCGGCCGTGAGCGCCACGCTGCGCCGCCAGGGCGTGATGATCACCAAGGTGA
AGAAGCGCCGGATGTCGGGTGGCAAGGCCATCAAGCAGAAGGACATCGCGATCTTCACCCGCCAGCTGGCCACGATGATG
AAGGCGGGCGTGCCGCTGCTGCAGGCCTTCGACATCGTCGGGCGTGGCAGCTCCAACCCCAAGCTCACCCGGCTGCTGAA
CGACGTGCGCTCCGACGTGGAGACCGGCACCAGCCTGTCGGCCGCGTTCCGCAAGCATCCGCTGTACTTCAATGCGCTGT
ATGCCAACCTGGTGGAAGCCGGCGAGGCCGGCGGCATCCTGGAGCAGCTGCTGGAGCGGCTGGCGCTGTACGAGGAGAAG
ACCCTCGCGCTGAAGTCCAAGATCAAGTCGGCGCTGATGTACCCGGTGGCGGTGATGGTGGTGGCCTTCATCGTGCTGAC
GGTGATCATGCTGTTCGTGATCCCGGCCTTCAAGGACGTGTTCAGCTCCTTCGGCGCTGACCTGCCGGCGCCCACGCTGG
CGGTGATCGCGATGTCGGAGTTCTTCGTCTCGTACTGGTGGCTCATCTTCGGCGTGATGTTCGGCGGCGGCTACTTCTTC
ATGCAGAGCTGGCGCCGCAGCGAGAAGATGCAGATGGCCATGGACCGGTTGCTGCTGCGCATCCCGGTGTTCGGCGACCT
GATCAACAAGAGCGTGATCGCCCGCTGGACGCGCACGCTGTCCACCATGTTCGCGGCCGGCGTGCCGCTGGTGGAGGCGC
TCGACTCGGTGGGCGGGGCCGCCGGCAATGCGGTGTACGCCGAGGCCACCGAGAAGATCCAGAAGGACGTGTCCACCGGC
TCTGCACTGACCAATGCGATGCAGGCCACCGGAGTGTTCCCGACCATGGTGCTGCAGATGGCCTCCATCGGCGAAGAATC
CGGCTCGCTGGACCACATGCTGGGCAAGGCGGCCGAGTTCTACGAGGACGAGGTCGACGAGATGGTCAAGGGCCTGTCCA
GCCTGATGGAGCCGTTCATCATCGTCATCCTGGGTACGCTGATCGGCGGTATCGTGGTGTCGATGTACCTGCCCATCTTC
AAGCTCGGCTCGGTCGTCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.208

99.507

0.539
  pilG Neisseria gonorrhoeae MS11

52.5

98.522

0.517
  pilG Neisseria meningitidis 44/76-A

52.25

98.522

0.515
  pilC Legionella pneumophila strain ERS1305867

50.758

97.537

0.495
  pilC Acinetobacter baylyi ADP1

49.497

98.03

0.485
  pilC Acinetobacter baumannii D1279779

47.607

97.783

0.466
  pilC Vibrio cholerae strain A1552

40.852

98.276

0.401
  pilC Vibrio campbellii strain DS40M4

40.5

98.522

0.399
  pilC Thermus thermophilus HB27

38.038

100

0.392