Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MW290_RS28280 Genome accession   NZ_CP097636
Coordinates   3019229..3020953 (+) Length   574 a.a.
NCBI ID   WP_250200122.1    Uniprot ID   -
Organism   Aquincola tertiaricarbonis strain RN12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3014229..3025953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW290_RS28250 (MW290_28245) proB 3014932..3016050 (-) 1119 WP_250197685.1 glutamate 5-kinase -
  MW290_RS28255 (MW290_28250) cgtA 3016043..3017113 (-) 1071 WP_250197686.1 Obg family GTPase CgtA -
  MW290_RS28260 (MW290_28255) rpmA 3017201..3017458 (-) 258 WP_250197687.1 50S ribosomal protein L27 -
  MW290_RS28265 (MW290_28260) rplU 3017488..3017799 (-) 312 WP_250197688.1 50S ribosomal protein L21 -
  MW290_RS28270 (MW290_28265) ispB 3018030..3018971 (+) 942 WP_250200121.1 octaprenyl diphosphate synthase -
  MW290_RS28280 (MW290_28275) pilF 3019229..3020953 (+) 1725 WP_250200122.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MW290_RS28285 (MW290_28280) pilC 3020974..3022194 (+) 1221 WP_250197689.1 type II secretion system F family protein Machinery gene
  MW290_RS28290 (MW290_28285) - 3022202..3023134 (+) 933 WP_250200123.1 A24 family peptidase -
  MW290_RS28295 (MW290_28290) coaE 3023142..3023756 (+) 615 WP_250197690.1 dephospho-CoA kinase -
  MW290_RS28300 (MW290_28295) zapD 3023784..3024539 (+) 756 WP_250197691.1 cell division protein ZapD -
  MW290_RS28305 (MW290_28300) - 3024536..3024745 (+) 210 WP_250197692.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62603.81 Da        Isoelectric Point: 5.7990

>NTDB_id=691374 MW290_RS28280 WP_250200122.1 3019229..3020953(+) (pilF) [Aquincola tertiaricarbonis strain RN12]
MPVDTVADTAQSSLSGVARVLVHAGRLQPRQAEALAAQARERRISFVSALIAANAVSASDLAHTLSNALAVPLLDLKSVD
AERLPRNVIDGKLATQYQVVVLGRRGNRLFIGGADPTDQEAIERIKFATQLSPEWVLVEYDKLGALLEAQGASVNEALDS
LAGGDFDFDVSDDAAAEDTANEVVSEVEDAPVVRFLQKMLIDAINARASDLHFEPYEYHYRVRFRIDGELREITQPPVAI
KDKLASRIKVISRLDISEKRVPQDGRMKLKFGNKAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEKIEKERLLH
AIQRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATVEDPAEINLPGINQVNVNEKAGMTFSVALKSFLRQDPDIIM
VGEIRDLETADIAIKAAQTGHLVMSTLHTNDAPTTLTRLLNMGVAPFNIASSVLIITAQRLARRLCENCKAPAEYPRDAL
KRAGFRDEDLDGTWKPYRAIGCSACNNGYKGRVGLYQVMPITEAIQRIILAEGTALDIAKQAQLEGVRDLRQAGLVKVRA
GVTTLEEVITVTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=691374 MW290_RS28280 WP_250200122.1 3019229..3020953(+) (pilF) [Aquincola tertiaricarbonis strain RN12]
CTGCCTGTGGACACCGTCGCTGACACTGCCCAATCGAGCCTGTCCGGAGTCGCCCGCGTGCTGGTGCATGCGGGCCGCCT
GCAGCCGCGCCAGGCCGAGGCACTCGCCGCGCAGGCGCGTGAGCGGCGCATCAGCTTCGTCTCGGCGCTGATCGCGGCCA
ACGCCGTCTCGGCGTCGGACCTGGCGCACACCTTGTCCAATGCGCTGGCGGTGCCGCTGCTCGACCTCAAGTCGGTGGAT
GCCGAGCGCCTGCCGCGCAACGTGATCGACGGCAAGCTGGCCACGCAGTACCAGGTGGTGGTGCTGGGCCGGCGGGGCAA
TCGCCTGTTCATCGGCGGGGCCGACCCCACCGACCAGGAAGCGATCGAGCGCATCAAGTTCGCCACCCAGCTGTCGCCCG
AGTGGGTGCTGGTGGAGTACGACAAGCTGGGCGCGCTGCTGGAAGCCCAGGGCGCCAGCGTCAACGAGGCGCTGGACAGC
CTGGCCGGCGGCGACTTCGACTTCGACGTCAGCGACGACGCGGCGGCCGAGGACACGGCCAACGAGGTGGTGTCGGAGGT
GGAGGACGCGCCGGTGGTGCGCTTCCTGCAGAAGATGCTGATCGACGCGATCAACGCGCGCGCCTCCGACCTGCACTTCG
AGCCCTACGAATACCACTACCGCGTGCGCTTCCGCATCGACGGCGAGCTGCGCGAGATCACGCAGCCGCCGGTGGCCATC
AAGGACAAGCTGGCCTCGCGCATCAAGGTGATCAGCCGGCTCGACATCTCCGAAAAACGCGTGCCGCAGGACGGCCGCAT
GAAGCTGAAGTTCGGCAACAAGGCGATCGACTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGATCGTGATCC
GGATCCTGGACCCGTCCTCGGCCAAGCTGGGCATCGAGGCGCTGGGCTACGAGAAGATCGAGAAGGAACGGCTGCTGCAC
GCCATCCAGCGGCCCTACGGGATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTCTACACCTGCCT
GAACCTGCTGAACCAGCCGGGCGTCAACATCGCCACGGTGGAAGATCCGGCGGAAATCAACCTGCCCGGCATCAACCAGG
TGAACGTGAACGAGAAGGCCGGCATGACCTTCTCGGTGGCGCTGAAGTCCTTCCTGCGGCAGGACCCGGACATCATCATG
GTGGGCGAGATCCGCGACCTGGAAACCGCCGACATCGCGATCAAGGCCGCGCAGACCGGCCACCTGGTGATGTCGACGCT
GCACACCAATGACGCGCCCACCACGCTGACGCGGCTGCTCAACATGGGCGTGGCGCCGTTCAACATCGCCTCCAGCGTGC
TGATCATCACCGCGCAGCGGCTGGCGCGGCGGCTGTGCGAGAACTGCAAGGCACCCGCCGAATATCCGCGAGACGCACTC
AAGCGCGCGGGCTTTCGTGACGAAGACCTCGATGGCACCTGGAAGCCGTACCGGGCGATCGGCTGCTCGGCCTGCAACAA
CGGCTACAAGGGCCGCGTCGGGCTGTACCAGGTGATGCCGATCACCGAAGCGATCCAACGCATCATCCTGGCCGAAGGCA
CGGCACTGGACATCGCCAAGCAGGCGCAGCTGGAAGGTGTCCGTGACCTGCGGCAGGCCGGCCTGGTGAAGGTGCGGGCC
GGGGTGACGACGCTGGAAGAGGTGATCACGGTCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.996

98.084

0.53
  pilB Acinetobacter baylyi ADP1

52.456

99.303

0.521
  pilB Acinetobacter baumannii D1279779

52.575

98.084

0.516
  pilB Legionella pneumophila strain ERS1305867

50.174

100

0.502
  pilB Vibrio cholerae strain A1552

45.922

98.258

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

45.293

98.084

0.444
  pilB Vibrio campbellii strain DS40M4

44.84

97.909

0.439