Detailed information NTG0069067

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: STO1_RS05970
• Genome accession: NZ_AP018338
• Coordinates: 1181462..1182112 (-)
• Length: 216 a.a.
• NCBI ID: WP_061587623.1
• Uniprot ID: A0A1X1FY60
• Organism: Streptococcus oralis subsp. tigurinus strain osk_001
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1176462..1187112

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
STO1_RS05950 (STO1_011590)	-	1177058..1178241 (-)	1184	Protein_1164	hypothetical protein	-
STO1_RS05955 (STO1_011610)	-	1178238..1178831 (-)	594	WP_061588100.1	ATP-binding cassette domain-containing protein	-
STO1_RS09920	-	1178863..1179090 (-)	228	WP_061588099.1	hypothetical protein	-
STO1_RS05965 (STO1_011630)	comEC/celB	1179238..1181478 (-)	2241	WP_096422408.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
STO1_RS05970 (STO1_011640)	comEA/celA/cilE	1181462..1182112 (-)	651	WP_061587623.1	helix-hairpin-helix domain-containing protein	Machinery gene
STO1_RS05975 (STO1_011650)	-	1182179..1182748 (-)	570	WP_084939084.1	GNAT family N-acetyltransferase	-
STO1_RS05985 (STO1_011660)	ald	1182925..1184037 (+)	1113	WP_045616943.1	alanine dehydrogenase	-
STO1_RS05990 (STO1_011670)	-	1184087..1185073 (-)	987	WP_000658201.1	PhoH family protein	-
STO1_RS05995 (STO1_011680)	-	1185154..1185369 (-)	216	WP_001232084.1	YozE family protein	-
STO1_RS06000 (STO1_011690)	-	1185393..1185971 (-)	579	WP_061588095.1	GrpB family protein	-
STO1_RS06005 (STO1_011700)	cvfB	1185983..1186837 (-)	855	WP_096422410.1	RNA-binding virulence regulatory protein CvfB	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23199.37 Da
• Isoelectric Point: 5.9270

>NTDB_id=69067 STO1_RS05970 WP_061587623.1 1181462..1182112(-) (comEA/celA/cilE) [Streptococcus oralis subsp. tigurinus strain osk_001]
MEAIIEKIKEYKIIAICACLGMGLGGFFLLKPSSQTSMKETNLQAEVAAVSKDSSSEKEVKKEEKEESTEQDLITVDVKG
AVKSPGIYDLPVGSRVHDAVQKAGGLTEEADSKSLNLAQKVSDEALVYVPTKGEEAASQQAASGTTPSTSKEKKVNLNRA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=69067 STO1_RS05970 WP_061587623.1 1181462..1182112(-) (comEA/celA/cilE) [Streptococcus oralis subsp. tigurinus strain osk_001]
ATGGAAGCAATTATCGAGAAAATCAAAGAATATAAAATCATTGCCATCTGTGCTTGTTTGGGTATGGGCTTAGGAGGCTT
TTTCCTGCTAAAGCCAAGTTCACAAACATCTATGAAAGAAACGAATTTGCAGGCTGAAGTTGCAGCCGTTTCAAAGGATT
CATCGTCTGAAAAAGAAGTGAAGAAGGAGGAAAAGGAAGAATCTACTGAACAAGATCTAATCACAGTGGATGTCAAAGGT
GCCGTTAAATCGCCAGGAATTTATGATTTGCCAGTTGGCAGTCGAGTTCATGATGCTGTTCAGAAAGCTGGTGGATTGAC
AGAGGAGGCAGATAGCAAATCGCTAAATCTCGCTCAGAAAGTCAGTGACGAGGCTCTTGTCTATGTTCCAACTAAGGGAG
AAGAAGCAGCTAGTCAGCAGGCTGCCTCTGGAACGACTCCTTCAACAAGTAAAGAAAAGAAGGTCAACCTCAATAGAGCT
AGTCTGGAAGAACTCAAGCAGGTCAAAGGCTTGGGAGGAAAACGAGCTCAGGATATTATCGACCATCGCGAGGCAAATGG
CAAGTTCAAGTCGGTTGATGAACTAAAGAAAGTTTCTGGCATTGGCGCAAAGACCATAGAAAAGCTAAAAGATTATGTCA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (151-214)

3D structure

• AlphaFold DB: A0A1X1FY60

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus mitis NCTC 12261	90.278	100	0.903
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	89.815	100	0.898
comEA/celA/cilE	Streptococcus pneumoniae D39	87.963	100	0.88
comEA/celA/cilE	Streptococcus pneumoniae R6	87.963	100	0.88
comEA/celA/cilE	Streptococcus pneumoniae Rx1	87.963	100	0.88
comEA/celA/cilE	Streptococcus mitis SK321	85.648	100	0.856
comEA	Lactococcus lactis subsp. cremoris KW2	39.111	100	0.407
comEA	Latilactobacillus sakei subsp. sakei 23K	33.906	100	0.366
comEA	Staphylococcus aureus MW2	36.073	100	0.366