Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   M5D45_RS14610 Genome accession   NZ_CP097330
Coordinates   3091897..3093162 (+) Length   421 a.a.
NCBI ID   WP_144201287.1    Uniprot ID   A0AAE9I4A4
Organism   Cupriavidus campinensis strain G5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3086897..3098162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5D45_RS14580 (M5D45_14580) rpmA 3087641..3087901 (-) 261 WP_061955320.1 50S ribosomal protein L27 -
  M5D45_RS14585 (M5D45_14585) rplU 3087955..3088266 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  M5D45_RS14590 (M5D45_14590) - 3088542..3088826 (-) 285 WP_144201293.1 hypothetical protein -
  M5D45_RS14595 (M5D45_14595) - 3088804..3089733 (+) 930 WP_144201291.1 polyprenyl synthetase family protein -
  M5D45_RS14605 (M5D45_14605) pilB 3090134..3091860 (+) 1727 Protein_2872 type IV-A pilus assembly ATPase PilB -
  M5D45_RS14610 (M5D45_14610) pilG 3091897..3093162 (+) 1266 WP_144201287.1 type II secretion system F family protein Machinery gene
  M5D45_RS14615 (M5D45_14615) pilD 3093166..3094032 (+) 867 WP_144201285.1 prepilin peptidase Machinery gene
  M5D45_RS14620 (M5D45_14620) coaE 3094087..3094695 (+) 609 WP_144201283.1 dephospho-CoA kinase -
  M5D45_RS14625 (M5D45_14625) zapD 3094896..3095654 (+) 759 WP_144201281.1 cell division protein ZapD -
  M5D45_RS14630 (M5D45_14630) - 3095665..3095856 (+) 192 WP_144201279.1 DNA gyrase inhibitor YacG -
  M5D45_RS14635 (M5D45_14635) - 3095886..3097928 (-) 2043 WP_144201277.1 OPT family oligopeptide transporter -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46447.67 Da        Isoelectric Point: 10.1574

>NTDB_id=688629 M5D45_RS14610 WP_144201287.1 3091897..3093162(+) (pilG) [Cupriavidus campinensis strain G5]
MATRAPAAGARTAAPSRAKSGRKAPTQYIFEWEAKDRKGKGFKGEQRAESQAEVVATLRKQGLTVVKMKKRKAARGKKIT
EKDIAYFTRQLSTMLKAGIPLLQSIDIIARGHANPNFTQLLSDIRFDIEAGSSMATAFRRQPKYFDTLYCNLIDAGEQGG
ILDALLERLSLYMEKTIALKSQIKSAMIYPISVLCVAFAVTVVLMLFVIPAFKGVFSSFGAALPAPTMVVINMSDFFVTY
WYLVIGIPIASIIFYIRARKTSEKVQRWHDKALLKLPIFGSLFRKAVIARWTRTLATMFAAGTPLVESMESVAGAAGNWL
YYDATREIEQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVAAISSLIEPLIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=688629 M5D45_RS14610 WP_144201287.1 3091897..3093162(+) (pilG) [Cupriavidus campinensis strain G5]
ATGGCGACGCGCGCACCAGCGGCGGGCGCCCGGACGGCAGCGCCGTCGCGGGCCAAATCAGGGCGGAAGGCGCCCACGCA
ATACATCTTCGAGTGGGAAGCCAAGGACCGGAAGGGCAAGGGCTTCAAGGGCGAGCAACGGGCCGAAAGCCAGGCGGAGG
TCGTCGCCACGCTGCGCAAGCAGGGGCTGACCGTCGTCAAGATGAAGAAGCGCAAGGCGGCGCGCGGCAAGAAGATCACC
GAGAAGGATATTGCGTACTTCACGCGCCAGCTCTCGACCATGCTCAAGGCCGGCATTCCGCTGCTGCAATCGATCGACAT
CATCGCGCGCGGCCACGCCAACCCCAATTTCACCCAGCTGCTGTCCGACATCCGCTTCGACATCGAGGCCGGCAGCAGCA
TGGCCACGGCGTTCCGGCGCCAGCCGAAGTACTTCGACACGCTGTACTGCAACCTGATCGACGCGGGTGAGCAGGGCGGT
ATTCTCGATGCCCTGCTGGAGCGCCTTTCGCTCTACATGGAAAAGACCATCGCGCTGAAGAGCCAGATCAAGTCCGCGAT
GATCTACCCGATCTCGGTGCTCTGCGTGGCCTTCGCCGTGACCGTGGTGCTGATGCTGTTCGTGATTCCGGCGTTCAAGG
GCGTGTTCTCGAGCTTCGGCGCCGCCCTGCCTGCGCCGACGATGGTCGTGATCAACATGTCCGACTTCTTCGTCACGTAC
TGGTATCTGGTGATCGGGATACCGATTGCCTCGATCATCTTCTACATCCGGGCGCGCAAGACCTCCGAAAAGGTCCAGCG
CTGGCACGACAAGGCACTGCTGAAGCTGCCGATCTTCGGCAGCCTGTTCCGCAAGGCCGTGATCGCACGCTGGACCCGCA
CGCTGGCCACGATGTTCGCCGCCGGCACGCCGCTGGTGGAGTCGATGGAATCCGTGGCCGGCGCCGCCGGTAACTGGCTG
TATTACGATGCCACGCGGGAAATCGAACAGGCCGTGCGGATCGGTACCAGCCTGACCAACGCCATGCAGGCCACGCACGT
GTTCGACAACATGGTGCTCCAGATGACGCAGATTGGCGAGGAGTCTGGCGCGCTGGACAACATGCTGCTCAAGGTGGCGG
AATTCTACGAGCGCGAGGTCGATGACGCCGTGGCGGCCATCTCCAGCCTGATCGAGCCGCTGATCATCGTGATCCTGGGC
GTGCTGATTGGCGGCATGGTGGTGGCGATGTACCTGCCGATCTTCAAGCTGGGCCAGGTGGTGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

50.995

95.487

0.487
  pilG Neisseria meningitidis 44/76-A

50.746

95.487

0.485
  pilC Pseudomonas stutzeri DSM 10701

49.132

95.724

0.47
  pilC Acinetobacter baumannii D1279779

48.642

96.2

0.468
  pilC Acinetobacter baylyi ADP1

46.929

96.675

0.454
  pilC Legionella pneumophila strain ERS1305867

47.277

95.962

0.454
  pilC Vibrio campbellii strain DS40M4

38.462

95.724

0.368
  pilC Vibrio cholerae strain A1552

38.889

94.062

0.366
  pilC Thermus thermophilus HB27

36.779

98.812

0.363