Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   M4S28_RS14710 Genome accession   NZ_CP097293
Coordinates   3305351..3306580 (+) Length   409 a.a.
NCBI ID   WP_249632025.1    Uniprot ID   -
Organism   Vibrio sp. J383     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3300351..3311580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4S28_RS14690 (M4S28_14690) ampD 3301393..3301992 (-) 600 WP_017087406.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M4S28_RS14695 (M4S28_14695) nadC 3302076..3302966 (+) 891 WP_017079961.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M4S28_RS14700 (M4S28_14700) - 3303222..3303650 (+) 429 WP_249632023.1 pilin -
  M4S28_RS14705 (M4S28_14705) pilB 3303651..3305336 (+) 1686 WP_249632024.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M4S28_RS14710 (M4S28_14710) pilC 3305351..3306580 (+) 1230 WP_249632025.1 type II secretion system F family protein Machinery gene
  M4S28_RS14715 (M4S28_14715) pilD 3306648..3307517 (+) 870 WP_249632026.1 A24 family peptidase Machinery gene
  M4S28_RS14720 (M4S28_14720) coaE 3307520..3308134 (+) 615 WP_017079956.1 dephospho-CoA kinase -
  M4S28_RS14725 (M4S28_14725) zapD 3308161..3308901 (+) 741 WP_017088714.1 cell division protein ZapD -
  M4S28_RS14730 (M4S28_14730) yacG 3308983..3309180 (+) 198 WP_017056605.1 DNA gyrase inhibitor YacG -
  M4S28_RS14735 (M4S28_14735) rplS 3309542..3309895 (-) 354 WP_004735514.1 50S ribosomal protein L19 -
  M4S28_RS14740 (M4S28_14740) trmD 3309955..3310695 (-) 741 WP_004735512.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  M4S28_RS14745 (M4S28_14745) rimM 3310722..3311276 (-) 555 WP_004735510.1 ribosome maturation factor RimM -
  M4S28_RS14750 (M4S28_14750) rpsP 3311301..3311549 (-) 249 WP_004735508.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 45383.31 Da        Isoelectric Point: 10.1823

>NTDB_id=688327 M4S28_RS14710 WP_249632025.1 3305351..3306580(+) (pilC) [Vibrio sp. J383]
MSSKSKQSQLKNYHWKGINSSGKKVSGQSLALTELEVREKLKEQHIQIKKIKKKSISAITRLTHRVKAKDITILTRQLAT
MLATGVPIVQAIKLVSDNHRKAEMKSILSHICKGVEAGTPISKAMRTASRHFDDLYTDLVATGELSGNLAQVFERLATYR
EKSEQLKSKVIKALIYPAMVVAVALTVSYLMLTMVIPEFESMFSGFGADLPWFTQQVLYLSHWMQAYSFYTAIGIGLLIL
SFQQLRQRSYSIRLSTSRLGLRFPILGAVITKASIAKFSRTLSTSFSSGIPILTSLKTTAKTAGNLHYESAIIEVHRETA
SGMPMYIAMRNTNAFPEMVLQMVMIGEESGDLDDMLNKVASIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLP
IFNLMSVLG

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=688327 M4S28_RS14710 WP_249632025.1 3305351..3306580(+) (pilC) [Vibrio sp. J383]
ATGAGTAGTAAAAGCAAACAATCACAACTAAAAAACTACCATTGGAAGGGAATCAATAGTTCTGGCAAGAAAGTATCAGG
GCAGAGCTTAGCGCTCACCGAATTAGAAGTGCGAGAGAAGCTCAAAGAGCAGCACATTCAGATTAAGAAAATCAAAAAGA
AAAGCATCTCAGCAATCACTCGTTTAACTCACCGCGTCAAAGCCAAAGATATCACTATTTTGACTCGCCAACTGGCTACC
ATGTTGGCGACTGGTGTGCCGATCGTGCAAGCCATCAAGCTGGTGTCAGACAATCACCGCAAAGCAGAAATGAAATCGAT
TTTATCGCACATCTGCAAAGGTGTAGAAGCCGGAACGCCAATCTCAAAAGCGATGCGAACCGCAAGCCGCCACTTTGATG
ACCTCTATACCGACTTGGTCGCGACAGGCGAACTCTCCGGCAACCTTGCACAAGTATTTGAACGCTTGGCGACCTATCGT
GAAAAGAGTGAGCAACTAAAATCCAAAGTTATAAAGGCGCTCATCTATCCTGCAATGGTTGTTGCGGTCGCTCTTACCGT
TTCCTACTTAATGCTGACCATGGTAATTCCGGAATTTGAATCGATGTTTTCAGGCTTCGGAGCAGACTTACCTTGGTTCA
CCCAGCAAGTGCTTTATCTTTCCCATTGGATGCAGGCTTACAGTTTCTATACTGCCATCGGTATTGGTTTGTTAATTCTG
TCTTTTCAGCAACTGCGTCAGCGATCCTACTCGATTCGACTTTCAACCAGTCGCCTTGGCTTACGCTTTCCTATCTTGGG
TGCTGTCATCACCAAGGCATCGATTGCAAAGTTCAGCCGAACACTTTCAACCAGTTTCAGTTCTGGAATTCCCATCTTAA
CCAGCCTTAAAACCACAGCTAAAACCGCAGGCAACCTGCATTATGAATCGGCCATCATTGAGGTTCACCGTGAGACTGCC
TCAGGCATGCCGATGTACATTGCAATGCGCAATACCAATGCCTTCCCGGAGATGGTTTTGCAGATGGTGATGATTGGTGA
AGAGTCAGGAGATCTTGATGATATGCTCAATAAAGTCGCTTCCATTTACGAGTTCGAAGTAGACAACACCGTCGATAACC
TAGGCAAGATTCTAGAGCCACTGATCATCGTATTTTTAGGCACCGTAGTTGGCGGACTTGTTGTCGCGATGTACTTACCG
ATCTTTAATCTTATGAGTGTGTTAGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(278-400)

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

80.75

97.8

0.79
  pilC Vibrio campbellii strain DS40M4

74.138

99.267

0.736
  pilC Acinetobacter baylyi ADP1

42.543

100

0.425
  pilC Acinetobacter baumannii D1279779

42.574

98.778

0.421
  pilC Legionella pneumophila strain ERS1305867

41.855

97.555

0.408
  pilG Neisseria gonorrhoeae MS11

41.542

98.289

0.408
  pilG Neisseria meningitidis 44/76-A

41.294

98.289

0.406
  pilC Pseudomonas stutzeri DSM 10701

40.594

98.778

0.401