Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M4S28_RS14705 Genome accession   NZ_CP097293
Coordinates   3303651..3305336 (+) Length   561 a.a.
NCBI ID   WP_249632024.1    Uniprot ID   -
Organism   Vibrio sp. J383     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3298651..3310336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4S28_RS14685 (M4S28_14685) pdhR 3300151..3300912 (-) 762 WP_004735446.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  M4S28_RS14690 (M4S28_14690) ampD 3301393..3301992 (-) 600 WP_017087406.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M4S28_RS14695 (M4S28_14695) nadC 3302076..3302966 (+) 891 WP_017079961.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M4S28_RS14700 (M4S28_14700) - 3303222..3303650 (+) 429 WP_249632023.1 pilin -
  M4S28_RS14705 (M4S28_14705) pilB 3303651..3305336 (+) 1686 WP_249632024.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M4S28_RS14710 (M4S28_14710) pilC 3305351..3306580 (+) 1230 WP_249632025.1 type II secretion system F family protein Machinery gene
  M4S28_RS14715 (M4S28_14715) pilD 3306648..3307517 (+) 870 WP_249632026.1 A24 family peptidase Machinery gene
  M4S28_RS14720 (M4S28_14720) coaE 3307520..3308134 (+) 615 WP_017079956.1 dephospho-CoA kinase -
  M4S28_RS14725 (M4S28_14725) zapD 3308161..3308901 (+) 741 WP_017088714.1 cell division protein ZapD -
  M4S28_RS14730 (M4S28_14730) yacG 3308983..3309180 (+) 198 WP_017056605.1 DNA gyrase inhibitor YacG -
  M4S28_RS14735 (M4S28_14735) rplS 3309542..3309895 (-) 354 WP_004735514.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62032.62 Da        Isoelectric Point: 4.8705

>NTDB_id=688326 M4S28_RS14705 WP_249632024.1 3303651..3305336(+) (pilB) [Vibrio sp. J383]
MLTNLPTVLRQASLISLTQEQAVAEHVQASGISTPEALLVLEFFSGDSLASNIKTIFGLPLVQLASTDYETLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELESAIRKLYGRSISGQDSKRKDITQDELAN
LVEISDDEITSIEDLSQDDSPVSRFINQILVDAARKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLSHMGIEPFNLASSLSLIIAQRLARRLCDHCKAADDSPDIFLRHSIPNSQI
IYKASSQGCNECNQGYSGRVGIYEVMPFSDQLKNSLIDKPNALAIENLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=688326 M4S28_RS14705 WP_249632024.1 3303651..3305336(+) (pilB) [Vibrio sp. J383]
GTGCTAACCAACCTCCCAACCGTTCTACGTCAGGCTAGTTTAATTAGCCTGACACAAGAACAAGCTGTTGCGGAACATGT
ACAAGCTTCGGGTATTTCTACACCTGAAGCTTTGCTCGTTCTGGAGTTCTTTAGCGGTGATTCCTTAGCAAGCAATATCA
AAACCATTTTCGGTTTACCGTTAGTACAACTAGCTAGTACTGACTACGAAACCTTGTGTGAGCAATTGGGGCTTCGTGAG
CTGATCACCAAATATCGAGCAATCCCGATTTCAGTTTCTAGTTCTACTCTTACGCTCGCCTCGGCTGATCCGACCGACTT
ACAAGCTGAAGATGACTTTCGATTCGCAACCGGATTACAGATTGAATTGGTTGTCGCTAACTACTCTGAATTAGAAAGTG
CAATACGAAAGCTGTATGGCCGTTCGATTTCCGGGCAAGACTCAAAGCGCAAAGATATCACCCAAGATGAGCTCGCTAAC
CTTGTTGAGATATCTGATGACGAGATAACGTCCATTGAAGACCTCAGCCAAGATGACTCCCCTGTCAGTCGCTTTATCAA
TCAGATTCTAGTCGATGCAGCACGTAAAGGCGCATCGGACATCCACTTCGAACCTTATGAAGAAAACTACCGCGTACGTC
TACGTTGCGATGGCATCCTTGTCGAAATTCAACAGCCCGCCTCTCATTTAAGTCGTCGTCTATCGGCTCGCCTGAAGATC
CTCGCCAAACTTGATATCGCCGAACGTCGTTTGCCTCAAGATGGTCGTATCAAACTGCGCTTAAATGACGAACTGGCAAT
TGATATGCGTGTATCTACACTGCCAACATTATGGGGAGAAAAGATCGTACTGCGTCTTTTAGACAGCAGCGCTGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGATCAAAAAGCACTCTACCTTAATGCATTGAAACGCCCGCAAGGTATGATC
TTAATGACCGGTCCAACCGGCAGCGGTAAAACGGTTTCTCTCTATACTGGCCTTCGCGTTCTTAACACAACAGAACGCAA
CATCTCAACAGCCGAAGATCCTGTTGAAATTAACCTGTGTGGCATCAATCAAGTACAAGTGACGCCTAAGATCGGCTTTG
GTTTCGCTGAAGCGTTGCGGTCATTTTTACGACAAGATCCTGATGTGGTAATGGTCGGTGAGATCCGCGACTTGGAAACC
GCAGAGATCGCGATCAAAGCCTCACAAACCGGACACTTAGTGCTTTCGACACTGCACACCAATTCTGCAGCAGAAACCGT
GACTCGCCTCTCTCATATGGGGATAGAACCTTTTAACCTCGCTTCATCATTAAGTTTAATTATCGCCCAACGACTGGCAA
GACGATTGTGCGACCACTGTAAAGCCGCTGATGACTCACCTGATATATTTCTACGCCACTCTATTCCCAATAGCCAAATC
ATCTACAAAGCCAGCTCACAAGGATGTAATGAGTGTAATCAGGGATACTCGGGGCGAGTCGGTATCTATGAAGTCATGCC
GTTTAGCGACCAACTGAAAAATAGCCTGATCGACAAACCGAACGCGTTAGCCATTGAAAACTTGGCACGTCGAGAAGGCA
TGAGAACTTTACAAGAGTCAGGTCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.227

100

0.736
  pilB Vibrio campbellii strain DS40M4

70.285

100

0.704
  pilB Vibrio parahaemolyticus RIMD 2210633

69.751

100

0.699
  pilB Legionella pneumophila strain ERS1305867

47.645

98.396

0.469
  pilB Acinetobacter baumannii D1279779

50.192

93.048

0.467
  pilB Acinetobacter baylyi ADP1

49.326

92.513

0.456
  pilF Neisseria gonorrhoeae MS11

43.034

100

0.435