Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PDPUS_RS14530 Genome accession   NZ_AP018045
Coordinates   2966324..2968051 (-) Length   575 a.a.
NCBI ID   WP_371332465.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. piscicida strain 91-197     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2961324..2973051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PDPUS_RS22200 - 2961648..2961788 (+) 141 WP_224756768.1 hypothetical protein -
  PDPUS_RS14505 - 2961772..2961909 (+) 138 WP_231965232.1 DUF2970 domain-containing protein -
  PDPUS_RS14510 (PDPUS_1_03020) dam 2961893..2962735 (-) 843 WP_086957324.1 adenine-specific DNA-methyltransferase -
  PDPUS_RS14515 (PDPUS_1_03021) - 2962790..2964352 (-) 1563 WP_086957325.1 SPOR domain-containing protein -
  PDPUS_RS14520 (PDPUS_1_03022) aroB 2964367..2965446 (-) 1080 WP_086957326.1 3-dehydroquinate synthase -
  PDPUS_RS14525 (PDPUS_1_03023) aroK 2965476..2965994 (-) 519 WP_036765246.1 shikimate kinase AroK -
  PDPUS_RS14530 (PDPUS_1_03024) pilQ 2966324..2968051 (-) 1728 WP_371332465.1 type IV pilus secretin PilQ Machinery gene
  PDPUS_RS14535 (PDPUS_1_03025) - 2968075..2968479 (-) 405 WP_224756679.1 pilus assembly protein PilP -
  PDPUS_RS14540 (PDPUS_1_03027) - 2968598..2969195 (-) 598 Protein_2746 type 4a pilus biogenesis protein PilO -
  PDPUS_RS14545 (PDPUS_1_03028) - 2969192..2969833 (-) 642 WP_044174099.1 PilN domain-containing protein -
  PDPUS_RS14550 (PDPUS_1_03029) pilM 2969830..2970720 (-) 891 WP_224756732.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62770.28 Da        Isoelectric Point: 4.9659

>NTDB_id=68820 PDPUS_RS14530 WP_371332465.1 2966324..2968051(-) (pilQ) [Photobacterium damselae subsp. piscicida strain 91-197]
MKRMLSVSLGWQRCWVVMLCCWLGISQAAIAAGMNIVNNQITDLDFKRDNRGRGVLTVQLAKPQSLVDMRRSGKQLHIDI
FDTAIKDDMLYVLDVVDFATVVRSIETFRTEKGSKLILNMAGVFDYDYQLKGSRLEVVLNAKKKKTSSTAKAKQGKIDYK
GKPISINFQDVPVRNVLQLVAEYNDFNLVVSDTVQGNVTLRLDDVPWPQVLDIILQSKGLDKRVQGSVVLIAPKSELATN
ERQEMEAKRKEDELASLRSELIQINYAKSGDIADLLLGTKDGVSMLSQRGSLHVDERTNAIIIKDIPESIDSIEEIITAL
DIPVKQVQIEARIVTVDEGDIDELGVRWGVVNQNGSTTIGGSIEGNLASSGLLGDDATVDDFLNVNLGAITPGASSIAFQ
VAKLGDILLDLELSALQAESKAEIISSPRLVTTNKKAAYIEQGTEIPYLEAASSGAVSVDFKKAVLSLMVTPQITPDDKL
VLDLLVTQDKVGETVVTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIYQHQVNNSVRKVPLLGDIPGLGVLFRHKLQEMG
KRELLIFVTPKIITQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=68820 PDPUS_RS14530 WP_371332465.1 2966324..2968051(-) (pilQ) [Photobacterium damselae subsp. piscicida strain 91-197]
ATGAAAAGGATGTTATCGGTCAGCTTGGGTTGGCAACGTTGTTGGGTAGTTATGTTGTGCTGCTGGCTTGGTATCTCCCA
AGCTGCCATTGCTGCTGGCATGAATATTGTGAATAACCAAATCACCGACTTAGATTTTAAGCGTGATAATCGTGGACGAG
GCGTGCTCACTGTTCAGTTAGCCAAACCTCAATCTCTGGTTGATATGCGCCGTTCAGGGAAGCAATTACATATCGATATC
TTTGATACCGCGATTAAAGATGACATGTTGTATGTTCTCGATGTAGTCGACTTTGCAACGGTTGTTCGTTCTATTGAAAC
ATTTCGCACCGAGAAAGGCAGTAAGCTGATTCTCAATATGGCTGGCGTGTTTGATTATGATTACCAATTAAAAGGCTCCC
GTCTTGAAGTTGTTTTGAACGCCAAGAAAAAGAAAACCAGTAGTACTGCCAAGGCTAAGCAAGGCAAAATTGATTATAAA
GGTAAGCCCATTTCCATTAACTTCCAAGATGTTCCTGTGCGTAACGTACTGCAGTTGGTTGCCGAATATAATGATTTTAA
TCTCGTGGTTTCCGATACCGTGCAGGGTAATGTGACCTTACGTCTTGATGATGTGCCTTGGCCACAAGTGCTCGATATTA
TTTTGCAATCAAAAGGGTTGGATAAACGAGTTCAAGGTTCTGTGGTACTGATTGCGCCAAAATCTGAGTTGGCAACCAAT
GAGCGTCAAGAGATGGAAGCTAAACGTAAAGAAGACGAGTTAGCTTCTCTTCGTTCGGAGCTGATTCAAATTAATTACGC
TAAATCAGGAGATATCGCAGACCTTTTACTCGGAACGAAAGATGGGGTCAGTATGCTATCTCAACGTGGATCTTTGCATG
TTGATGAGCGTACTAATGCCATTATCATCAAAGATATCCCTGAGAGTATTGATAGCATTGAAGAGATTATCACAGCATTA
GATATTCCCGTTAAACAGGTTCAGATAGAAGCTCGTATTGTTACTGTTGATGAGGGGGATATTGACGAGTTAGGGGTACG
TTGGGGTGTCGTTAATCAAAACGGTAGTACAACCATTGGTGGCTCAATTGAAGGTAACCTGGCCAGTTCGGGCTTATTGG
GTGATGACGCGACTGTTGATGATTTCTTAAATGTAAATTTAGGAGCTATTACTCCAGGGGCATCGAGTATTGCATTTCAG
GTGGCAAAACTAGGCGATATTTTGCTCGATCTTGAATTATCCGCTCTACAGGCAGAATCAAAAGCAGAGATTATTTCTAG
TCCTCGATTAGTGACTACCAATAAAAAAGCGGCTTACATCGAACAAGGTACTGAGATCCCTTATTTAGAAGCGGCGTCCA
GTGGTGCGGTATCGGTAGACTTTAAAAAGGCCGTATTGAGTTTGATGGTCACACCACAAATTACCCCAGATGATAAATTG
GTGCTCGATCTGCTGGTCACGCAAGATAAAGTAGGGGAAACAGTTGTGACCGGAACCGGAGAAGCGGTCGCTATTGATAC
TCAACGAATTGGCACGCAAGTTCTGGTCAATAACGGCGAGACTGTGGTCTTAGGTGGTATCTACCAGCATCAGGTGAATA
ATTCTGTCCGTAAAGTCCCTTTGTTGGGTGATATTCCAGGATTGGGCGTATTGTTCCGTCATAAGCTGCAAGAGATGGGC
AAGCGTGAACTGTTGATTTTCGTCACACCAAAAATTATCACCCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(415-573)

Bacterial type II/III secretion system short domain (Secretin_N)

(260-326)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

60.946

99.304

0.605
  pilQ Vibrio cholerae strain A1552

60.946

99.304

0.605
  pilQ Vibrio campbellii strain DS40M4

60.823

97.217

0.591
  pilQ Legionella pneumophila strain ERS1305867

42.463

94.609

0.402
  pilQ Legionella pneumophila strain Lp02

42.463

94.609

0.402
  comQ Acinetobacter baylyi ADP1

40.212

98.609

0.397
  pilQ Pseudomonas aeruginosa PAK

40.787

97.217

0.397
  pilQ Acinetobacter baumannii D1279779

38.793

100

0.391
  pilQ Acinetobacter baumannii strain A118

38.793

100

0.391

Multiple sequence alignment