Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   M3N36_RS00595 Genome accession   NZ_CP097251
Coordinates   99374..100312 (+) Length   312 a.a.
NCBI ID   WP_030127399.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 21SPY7071 isolate cerebral abscess     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 94374..105312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3N36_RS00585 (M3N36_00585) rpoC 95122..98763 (+) 3642 WP_030127400.1 DNA-directed RNA polymerase subunit beta' -
  M3N36_RS00590 (M3N36_00590) - 98915..99280 (+) 366 WP_002986560.1 DUF1033 family protein -
  M3N36_RS00595 (M3N36_00595) comYA 99374..100312 (+) 939 WP_030127399.1 competence type IV pilus ATPase ComGA Machinery gene
  M3N36_RS00600 (M3N36_00600) comYB 100248..101282 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  M3N36_RS00605 (M3N36_00605) comYC 101284..101610 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  M3N36_RS00610 (M3N36_00610) comGD 101585..102013 (+) 429 WP_030127397.1 competence type IV pilus minor pilin ComGD -
  M3N36_RS00615 (M3N36_00615) comGE 101970..102254 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  M3N36_RS00620 (M3N36_00620) comYF 102247..102681 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  M3N36_RS00625 (M3N36_00625) comGG 102665..102991 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  M3N36_RS00630 (M3N36_00630) comYH 103089..104042 (+) 954 WP_030127396.1 class I SAM-dependent methyltransferase Machinery gene
  M3N36_RS00635 (M3N36_00635) - 104101..105297 (+) 1197 WP_011888536.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35058.36 Da        Isoelectric Point: 9.1037

>NTDB_id=688009 M3N36_RS00595 WP_030127399.1 99374..100312(+) (comYA) [Streptococcus pyogenes strain 21SPY7071 isolate cerebral abscess]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLVSHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQKA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGITKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=688009 M3N36_RS00595 WP_030127399.1 99374..100312(+) (comYA) [Streptococcus pyogenes strain 21SPY7071 isolate cerebral abscess]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTGTTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTCAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAAAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTATAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.415

99.679

0.612
  comYA Streptococcus mutans UA140

61.415

99.679

0.612
  comYA Streptococcus gordonii str. Challis substr. CH1

60.129

99.679

0.599
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60

99.359

0.596
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.199

99.679

0.58
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.199

99.679

0.58
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.199

99.679

0.58
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.199

99.679

0.58
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

40

92.949

0.372