Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   M3M30_RS00085 Genome accession   NZ_CP097161
Coordinates   21499..22713 (+) Length   404 a.a.
NCBI ID   WP_010961338.1    Uniprot ID   Q606C3
Organism   Methylococcus capsulatus strain MIR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16499..27713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3M30_RS00075 (M3M30_00075) - 16964..19729 (+) 2766 WP_249385295.1 valine--tRNA ligase -
  M3M30_RS00080 (M3M30_00080) pilB 19779..21494 (+) 1716 WP_010961339.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M3M30_RS00085 (M3M30_00085) pilC 21499..22713 (+) 1215 WP_010961338.1 type II secretion system F family protein Machinery gene
  M3M30_RS00090 (M3M30_00090) pilD 22737..23606 (+) 870 WP_218801400.1 A24 family peptidase Machinery gene
  M3M30_RS00095 (M3M30_00095) coaE 23614..24210 (+) 597 WP_218800346.1 dephospho-CoA kinase -
  M3M30_RS00100 (M3M30_00100) zapD 24366..25079 (+) 714 WP_017366377.1 cell division protein ZapD -
  M3M30_RS00105 (M3M30_00105) yacG 25093..25308 (+) 216 WP_017366376.1 DNA gyrase inhibitor YacG -
  M3M30_RS00110 (M3M30_00110) cysG 25321..26745 (-) 1425 WP_010961332.1 siroheme synthase CysG -
  M3M30_RS00115 (M3M30_00115) - 26794..27024 (+) 231 WP_010961331.1 sulfurtransferase TusA family protein -
  M3M30_RS00120 (M3M30_00120) - 27033..27695 (-) 663 WP_010961330.1 HAD family hydrolase -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 44020.67 Da        Isoelectric Point: 9.8132

>NTDB_id=687507 M3M30_RS00085 WP_010961338.1 21499..22713(+) (pilC) [Methylococcus capsulatus strain MIR]
MANQETALFVWEGLDRNGSRTRGEISSRSEITARTELRRQGIRVVKIKKKPKPLFSGPRQKITPKHIAVFSRQLATMLSA
GVPLVQAFDIVGRGHDNPAMQDLLMSIKADVEAGTTLADALSKHPVHFDELFCNLVRAGEQAGVLETLLHKVADYKEKTE
SLKGKIKKALAYPIAVVVVAVIVTSILLIFVVPTFEDLFKSFGADLPAFTQMVINLSRWLRDWWYVVFGSLGVAAAAFVK
ARQRSPAFNHLLDRLALGLPVVGAILRKAAVARFARTLSTMSAAGVPLVEALQSVAGATGNIIYGEAVMRMREDVSTGQQ
LQMSMRQANLFPNMVMQMVAIGEESGALDSMLSKVADFYEEEVDNAVDSLSSLLEPLIMVVLGVLIGGLVIAMYLPIFKL
GSVV

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=687507 M3M30_RS00085 WP_010961338.1 21499..22713(+) (pilC) [Methylococcus capsulatus strain MIR]
GTGGCGAACCAGGAAACCGCTTTGTTCGTCTGGGAGGGCCTGGACAGGAACGGCAGCCGGACCCGGGGTGAAATCAGCTC
CCGGTCGGAGATCACGGCGCGCACGGAGCTGAGGCGCCAGGGCATCCGCGTCGTCAAGATCAAGAAGAAGCCCAAGCCCC
TCTTCAGTGGTCCACGCCAGAAGATCACGCCCAAGCACATCGCCGTGTTCAGCCGCCAGTTGGCGACCATGCTGTCGGCC
GGGGTTCCCCTGGTCCAGGCATTCGACATCGTGGGCCGCGGCCATGACAATCCGGCCATGCAGGATCTGCTGATGAGCAT
CAAGGCCGACGTGGAAGCCGGCACGACCCTGGCGGACGCCCTGTCGAAGCATCCGGTCCATTTCGACGAGCTTTTCTGTA
ATCTGGTCCGGGCCGGCGAACAGGCCGGCGTGCTGGAAACCTTGTTGCACAAGGTGGCGGACTACAAGGAAAAAACCGAG
TCCCTCAAGGGCAAGATCAAGAAGGCTTTGGCTTATCCGATCGCCGTCGTCGTGGTTGCGGTGATCGTGACGTCGATCTT
GCTGATTTTCGTGGTGCCGACCTTCGAGGATCTGTTCAAGAGCTTCGGCGCCGATCTGCCGGCCTTCACCCAGATGGTGA
TCAACCTTTCGCGTTGGCTGCGGGACTGGTGGTACGTGGTGTTCGGCTCGCTAGGCGTAGCCGCCGCGGCGTTCGTGAAG
GCCCGGCAGCGCTCGCCGGCGTTCAATCATCTGCTGGACCGGCTGGCCTTGGGGCTGCCGGTCGTCGGCGCCATCCTGCG
CAAGGCCGCCGTCGCGCGCTTCGCCCGGACCTTGTCCACCATGTCCGCGGCCGGCGTGCCCCTGGTCGAGGCGTTGCAAT
CGGTGGCGGGGGCGACCGGAAACATCATCTACGGTGAAGCGGTCATGCGTATGCGCGAAGACGTATCCACCGGCCAGCAG
CTCCAGATGTCGATGCGCCAGGCCAATCTGTTTCCCAACATGGTGATGCAGATGGTGGCGATTGGCGAGGAGTCGGGGGC
GCTCGACAGCATGCTGTCCAAGGTCGCCGATTTCTACGAGGAAGAAGTGGACAATGCCGTGGATTCGTTGAGCAGCCTGC
TCGAACCTTTGATCATGGTGGTTCTGGGCGTGTTGATCGGGGGGCTCGTGATCGCCATGTATCTGCCGATTTTCAAACTG
GGTTCCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q606C3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.5

99.01

0.599
  pilC Legionella pneumophila strain ERS1305867

61.111

98.02

0.599
  pilC Acinetobacter baumannii D1279779

58.438

98.267

0.574
  pilC Acinetobacter baylyi ADP1

57.32

99.752

0.572
  pilG Neisseria meningitidis 44/76-A

47.103

98.267

0.463
  pilG Neisseria gonorrhoeae MS11

47.103

98.267

0.463
  pilC Vibrio campbellii strain DS40M4

44.361

98.762

0.438
  pilC Vibrio cholerae strain A1552

43.434

98.02

0.426
  pilC Thermus thermophilus HB27

41.103

98.762

0.406