Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M3M30_RS00080 Genome accession   NZ_CP097161
Coordinates   19779..21494 (+) Length   571 a.a.
NCBI ID   WP_010961339.1    Uniprot ID   Q606C2
Organism   Methylococcus capsulatus strain MIR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 14779..26494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3M30_RS00065 (M3M30_00065) - 14945..16438 (+) 1494 WP_218800349.1 leucyl aminopeptidase -
  M3M30_RS00070 (M3M30_00070) - 16435..16893 (+) 459 WP_218800348.1 DNA polymerase III subunit chi -
  M3M30_RS00075 (M3M30_00075) - 16964..19729 (+) 2766 WP_249385295.1 valine--tRNA ligase -
  M3M30_RS00080 (M3M30_00080) pilB 19779..21494 (+) 1716 WP_010961339.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M3M30_RS00085 (M3M30_00085) pilC 21499..22713 (+) 1215 WP_010961338.1 type II secretion system F family protein Machinery gene
  M3M30_RS00090 (M3M30_00090) pilD 22737..23606 (+) 870 WP_218801400.1 A24 family peptidase Machinery gene
  M3M30_RS00095 (M3M30_00095) coaE 23614..24210 (+) 597 WP_218800346.1 dephospho-CoA kinase -
  M3M30_RS00100 (M3M30_00100) zapD 24366..25079 (+) 714 WP_017366377.1 cell division protein ZapD -
  M3M30_RS00105 (M3M30_00105) yacG 25093..25308 (+) 216 WP_017366376.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63100.82 Da        Isoelectric Point: 5.5480

>NTDB_id=687506 M3M30_RS00080 WP_010961339.1 19779..21494(+) (pilB) [Methylococcus capsulatus strain MIR]
MAAVSGVQQLSGLAKSLVKAQLLSEADAQAYFEDARRKNTPFVSYLVANKILDGLQIAQVAHQEFGLPLLDLDAVVFDIP
PTQWVGEKLIRRHHILPLLRRGNHLFVAVSDPTNFLGLDEITFQTRLVTDCVLVEENKLSRCIEAVLDAADTSLKQLLEE
DLDSLQITAGDDEGPHAEPEISGIDDAPIVRFVNRLLLDAVRKGASDIHIEPYEKFLRIRFRHDGILHEVANPPPALGVK
ICARIKVMSRMDIAEHRIPQDGRIKMTLSRSRAIDFRVNTCPTLFGEKIVLRILDPSSAQIGVEMLGFEPEQRDNFLRAL
EKPYGMILVTGPTGSGKTVTLYTGLNLLNRVEANISTVEDPVEITVPGINQVNVNYKTGLTFAEALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPAFNIASSVLLIMAQRLARRLCPRCKREEKVPPEVLLA
SGFREEDIGNFTLFGPAGCDQCVKGYKGRVGIYQVMPISEEINRIILEGGNVMALTEQAHAEGIADLRESGLKKVKAGIT
SLEEIDRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=687506 M3M30_RS00080 WP_010961339.1 19779..21494(+) (pilB) [Methylococcus capsulatus strain MIR]
ATGGCGGCGGTTTCCGGCGTCCAGCAGCTGAGCGGTCTGGCGAAAAGCCTGGTCAAGGCCCAGTTGCTTTCGGAGGCGGA
CGCCCAGGCGTACTTCGAGGATGCGCGCAGGAAGAACACCCCTTTCGTCAGCTATCTCGTCGCCAACAAGATCCTGGACG
GTCTCCAGATCGCCCAGGTCGCCCACCAGGAGTTCGGGCTGCCCCTGCTCGATCTGGACGCAGTCGTCTTCGACATTCCC
CCCACGCAGTGGGTCGGCGAGAAGCTGATACGCCGCCATCACATCCTGCCCTTGCTGCGACGGGGGAATCACCTCTTCGT
CGCGGTCTCGGATCCGACCAATTTCCTGGGACTGGATGAAATCACTTTCCAGACCCGTCTCGTCACCGACTGTGTTCTGG
TCGAGGAGAACAAGCTTTCGCGCTGCATCGAGGCGGTCCTGGATGCGGCGGATACCAGCCTGAAGCAGCTGCTCGAAGAG
GATCTGGACAGTCTGCAGATCACCGCCGGGGACGACGAAGGCCCGCACGCCGAGCCGGAAATCTCCGGTATCGACGATGC
GCCGATCGTGCGCTTCGTCAACCGCCTTCTGCTGGACGCGGTGCGGAAGGGGGCCTCGGACATCCATATCGAGCCTTACG
AAAAATTCCTGCGCATCCGCTTCCGGCACGACGGCATCCTGCATGAGGTGGCGAATCCGCCGCCTGCGCTGGGCGTCAAG
ATCTGCGCTCGCATCAAAGTGATGTCGCGCATGGATATCGCGGAACACCGCATTCCCCAGGACGGACGCATCAAGATGAC
GCTGTCGCGCTCCCGCGCCATCGATTTCCGCGTCAATACCTGTCCGACGCTGTTCGGGGAAAAGATCGTGCTGCGTATCC
TCGATCCGAGCTCGGCCCAGATCGGCGTCGAAATGCTGGGATTCGAGCCGGAACAGCGGGACAATTTCCTGCGTGCGCTG
GAAAAGCCCTATGGCATGATCCTGGTTACCGGCCCCACCGGCAGCGGTAAGACCGTTACCCTCTACACCGGTCTGAACCT
CCTCAACCGCGTCGAAGCGAACATCTCCACGGTGGAGGACCCGGTCGAGATCACAGTGCCAGGGATCAACCAGGTCAATG
TCAATTACAAGACCGGCCTCACTTTCGCCGAGGCGCTGCGGGCGTTTCTGCGCCAGGACCCCGACATCATCATGGTGGGC
GAGATCCGCGACCTGGAAACCGCCGAAATCGCCGTCAAGGCGGCCCAGACCGGGCATCTGGTGCTGTCGACCCTGCACAC
CAACGATGCCCCGCAGACCTTGAACCGTCTGATGCAGATGGGGATCCCCGCGTTCAACATCGCCTCCTCCGTGCTCCTGA
TCATGGCGCAGCGGCTGGCCCGCCGGCTTTGTCCGCGGTGCAAGCGGGAGGAGAAAGTGCCGCCCGAGGTCCTGCTCGCC
TCCGGCTTCCGGGAGGAAGACATCGGCAATTTCACGCTGTTCGGGCCGGCGGGCTGCGATCAATGTGTGAAGGGCTACAA
GGGGCGGGTCGGCATTTACCAGGTCATGCCGATTTCGGAAGAAATCAACCGCATCATCCTGGAGGGAGGGAACGTGATGG
CCCTGACCGAGCAGGCGCATGCGGAAGGTATCGCGGATCTGAGGGAGTCTGGCTTGAAGAAGGTGAAGGCGGGTATCACC
AGCCTCGAGGAAATCGACCGCGTGACGAGGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q606C2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.652

100

0.56
  pilB Acinetobacter baylyi ADP1

52.397

100

0.536
  pilB Acinetobacter baumannii D1279779

51.986

100

0.527
  pilB Vibrio cholerae strain A1552

48.845

98.599

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

48.221

98.424

0.475
  pilF Neisseria gonorrhoeae MS11

47.518

98.774

0.469
  pilB Vibrio campbellii strain DS40M4

47.163

98.774

0.466
  pilF Thermus thermophilus HB27

37.786

99.65

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

40.232

90.543

0.364