Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   M2J80_RS23990 Genome accession   NZ_CP097103
Coordinates   5291789..5293057 (-) Length   422 a.a.
NCBI ID   WP_161892176.1    Uniprot ID   A0AAX3KGC8
Organism   Pseudomonas sp. NY11382     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5286789..5298057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J80_RS23970 (M2J80_23955) - 5288118..5288336 (-) 219 WP_161892178.1 hypothetical protein -
  M2J80_RS23975 (M2J80_23960) - 5288466..5290055 (-) 1590 WP_054881140.1 AAA family ATPase -
  M2J80_RS23980 (M2J80_23965) aroB 5290071..5291168 (-) 1098 WP_161892177.1 3-dehydroquinate synthase -
  M2J80_RS23985 (M2J80_23970) aroK 5291266..5291784 (-) 519 WP_009684951.1 shikimate kinase AroK -
  M2J80_RS23990 (M2J80_23975) pilQ 5291789..5293057 (-) 1269 WP_161892176.1 type IV pilus secretin PilQ family protein Machinery gene
  M2J80_RS23995 (M2J80_23980) - 5292981..5293979 (-) 999 WP_161892187.1 pilus assembly protein PilP -
  M2J80_RS24000 (M2J80_23985) - 5293991..5294527 (-) 537 WP_054904626.1 PilN domain-containing protein -
  M2J80_RS24005 (M2J80_23990) pilM 5294524..5295405 (-) 882 WP_110677639.1 pilus assembly protein PilM -
  M2J80_RS24010 (M2J80_23995) - 5295584..5298037 (+) 2454 WP_161892175.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46021.83 Da        Isoelectric Point: 4.9201

>NTDB_id=687064 M2J80_RS23990 WP_161892176.1 5291789..5293057(-) (pilQ) [Pseudomonas sp. NY11382]
MRTRMIYLLAALLPMPLATATPYQGEPLSLHFQDVEIRSVLQVLADYAGVNLVASDEVQGNVTLRLQDVPWDQALDLVLR
SKSLARRQEGNVLLVAPAATLAAQTAEARAGQVLEAQLQPMRRELLPVHHAKAAELAELLKTSLADESLLASWGSLSVDE
RTNTLVAYLPAERLDELRQLVAQLDVPVRQVAIEARIVEANVDYEKALGVRWGSPLYGDNPLPGKSLFVDLGVERAGSSI
GLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETTKSGATSVSFREASLSLEVTPQITPDN
KVIMAVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFLGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=687064 M2J80_RS23990 WP_161892176.1 5291789..5293057(-) (pilQ) [Pseudomonas sp. NY11382]
ATGAGAACGAGGATGATCTACCTGCTGGCGGCCCTGCTGCCCATGCCTCTGGCGACCGCGACGCCTTACCAGGGTGAGCC
GCTGTCGCTGCACTTTCAGGATGTCGAGATTCGCTCGGTTTTGCAGGTACTCGCCGACTATGCCGGCGTCAACCTGGTCG
CCAGCGACGAGGTGCAGGGCAATGTCACCCTGCGCTTGCAGGATGTGCCGTGGGATCAGGCGCTGGACCTGGTCTTGCGC
AGCAAAAGCCTCGCCCGGCGCCAGGAGGGCAATGTGTTGCTGGTGGCGCCGGCGGCCACGCTGGCTGCGCAAACTGCCGA
AGCGCGTGCGGGGCAGGTGCTGGAGGCGCAGTTGCAGCCCATGCGCCGGGAGCTGTTGCCTGTTCACCATGCCAAGGCTG
CCGAACTTGCGGAACTGCTCAAGACCAGCCTGGCCGACGAAAGCCTGCTCGCCAGTTGGGGCAGCCTGAGTGTCGATGAG
CGCACCAATACCCTGGTGGCCTACCTGCCGGCCGAACGCCTCGATGAACTGCGGCAACTGGTGGCGCAGCTGGATGTGCC
GGTGCGTCAGGTGGCGATCGAGGCGCGCATCGTCGAGGCCAACGTCGATTACGAGAAAGCCCTTGGGGTGCGCTGGGGTA
GCCCTTTGTATGGCGACAACCCGCTGCCGGGCAAGTCGCTGTTCGTCGACCTGGGTGTCGAGCGGGCTGGCAGCAGCATC
GGCCTTGGCCTGCTGCGCGGGGATGTGCTGCTGGACCTCGAACTCAGCGCCATGGAAAAAAGCGGCAATGGCGAGATCAT
TTCGCAACCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAGGGCACCGAGGTGCCCTACCAGGAAACCA
CCAAGAGCGGCGCCACCTCGGTTTCGTTCCGCGAGGCCTCGCTGTCGTTGGAGGTGACCCCGCAAATTACCCCGGACAAC
AAGGTAATCATGGCTGTCAGGGTGACCAAGGACGAACCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTGAATGCCAAGGTTCGCGTGGCCGACGGCGAAACGATCGTGATTGGCGGCGTGTACTCGACCTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTGGGTGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.404

100

0.614
  comQ Acinetobacter baylyi ADP1

43.207

100

0.46
  pilQ Acinetobacter baumannii strain A118

42.082

100

0.46
  pilQ Acinetobacter baumannii D1279779

42.082

100

0.46
  pilQ Legionella pneumophila strain Lp02

42.584

99.052

0.422
  pilQ Legionella pneumophila strain ERS1305867

42.584

99.052

0.422
  pilQ Vibrio campbellii strain DS40M4

41.274

100

0.415
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.865

98.578

0.403
  pilQ Vibrio cholerae strain A1552

40.865

98.578

0.403
  pilQ Neisseria gonorrhoeae strain FA1090

36.364

99.052

0.36